Transcriptomic Analysis of Non-Specific Immune Responses in the Rice Field Eel (<i>Monopterus albus</i>) Infected with <i>Pallisentis (Neosentis) celatus</i>

Transcriptomic Analysis of Non-Specific Immune Responses in the Rice Field Eel (<i>Monopterus albus</i>) Infected with <i>Pallisentis (Neosentis) celatus</i>

Parasitic infestations present significant threats to the physiological health and ecological stability of aquatic species, frequently compromising immune defenses and elevating mortality rates. This study was conducted to elucidate the non-specific immune responses induced by <i>Pallisentis (...

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Main Authors: Qin Lei, Xiaoling Li, Hao Wu, Yiwen Wan, Yukun Xie, Jinwei Gao, Wenwen Suo, Ming Zeng, Lingli Liu, Dongsheng Ou, Zhonggui Xie, Rui Song
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Fishes
Subjects:
<i>Pallisentis (Neosentis) celatus</i>
<i>Monopterus albus</i>
intestinal
non-specific immune
Online Access:https://www.mdpi.com/2410-3888/9/11/452
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Summary:Parasitic infestations present significant threats to the physiological health and ecological stability of aquatic species, frequently compromising immune defenses and elevating mortality rates. This study was conducted to elucidate the non-specific immune responses induced by <i>Pallisentis (Neosentis) celatus</i> infection in <i>Monopterus albus</i>, with a focus on intestinal histopathology and transcriptome gene expression. A histopathological examination revealed minor alterations in intestinal villi under low-level infection. A transcriptome analysis, performed using Illumina sequencing technology, identified 347 upregulated and 298 downregulated genes involved in critical biological pathways, such as lipid metabolism, immune responses, and the regulation of inflammatory processes. GO and KEGG analyses indicated the upregulation of immune-related pathways, including the RIG-I-like and IL-17 signaling pathways, highlighting a robust intestinal immune response. Conversely, the complement pathway was found to be downregulated, with significant suppression of <i>C9</i>, suggesting that the parasite may engage in immune evasion. Fluorescein-labeled <i>C9</i> antibody assays confirmed reduced complement <i>C9</i> levels in the infected tissues. A real-time PCR analysis identified the differential expression of eight genes, including <i>C5</i>, <i>maats1</i>, <i>CFI</i>, and <i>gmnc</i>, which were consistent with the sequencing results. These findings suggest that <i>Pallisentis (Neosentis) celatus</i> infection compromises intestinal health, induces inflammation, and activates non-specific immune responses in <i>Monopterus albus</i>. However, <i>Pallisentis (Neosentis) celatus</i> appears to evade the host immune response by suppressing the activation of complement components, thereby facilitating its reproductive parasitism.
ISSN:2410-3888

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