THE HEAT SHOCK PROTEIN 70-2 GENE (RS1061581) ASSOCIATES WITH THE ACUTE OTITIS MEDIUM IN CHILDREN
Acute otitis media (AOM) represents one of the most prevalent pediatric conditions worldwide. Affecting millions of children / adolescents each year, AOM constitutes a significant public health concern. Although the fundamental mechanisms underlying AOM development are understood, the molecular and...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Bukovynian State Medical University
2025-04-01
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| Series: | Неонатологія, хірургія та перинатальна медицина |
| Subjects: | |
| Online Access: | https://neonatology.bsmu.edu.ua/article/view/326008 |
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| Summary: | Acute otitis media (AOM) represents one of the most prevalent pediatric conditions worldwide. Affecting millions of children / adolescents each year, AOM constitutes a significant public health concern. Although the fundamental mechanisms underlying AOM development are understood, the molecular and genetic aspects of its pathogenesis remain insufficiently explored.
The Aim of this study is to investigate the association between the HSP70-2 gene (rs1061581) A1267G polymorphism and AOM in children, as well as to evaluate the inheritance patterns of the disease and the risks associated with the severity of AOM in the studied cohort.
Materials and Methods. A prospective cohort study was conducted involving 95 children with AOM, aged 7 to 18 years. The cohort comprised 34.74% (n=33) girls and 65.26% (n=62) boys. The study adhered to the principles of Good Clinical Practice (GCP), Good Laboratory Practice (GLP), and biomedical ethics standards for scientific research involving human participants. Informed consent was obtained from the parents or guardians of each child. Participants were stratified by age (7–11 years (n=81) and 12–18 years (n=14)), AOM severity (severe – 45.26% (n=43), non-severe – 54.74% (n=52)), and the nature of mucosal inflammation (catarrhal – 52.63% (n=50), purulent – 47.37% (n=45)). A control group of 50 healthy children was included, consisting of 20 girls (40.0%) and 30 boys (60.0%). The groups were age-matched (p>0.05). The HSP70-2 (rs1061581) gene polymorphism was analyzed using qualitative polymerase chain reaction. Risk assessment was performed using Risk Ratio, Odds Ratio, and 95% Confidence Intervals.
Results. The mutation frequency of the heat shock protein gene HSP70-2 (rs1061581) in children with AOM from Northern Bukovina was 35.79% (8.42% in homozygous state), which was 11.79% higher than in healthy children (χ²=4.20; p=0.04). The distribution of HSP70-2 (rs1061581) genotypes in the studied cohort generally conformed to the Hardy-Weinberg equilibrium (χ²=1.36; p=0.243) and did not deviate in allele frequency from the average values observed in Caucasian populations (PA=0.24–0.36; PG=0.64–0.76). Binary logistic regression analysis proved a significant probability of AOM development in A-allele carriers' children under both dominant and additive models (OR=2.01; 95%CI: 1.01–4.06; p=0.05 and OR=2.01; 95%CI: 1.10–3.77; p=0.03), with the lowest out-of-sample Akaike prediction coefficients (AC=16.67 and 15.33).
Epidemiological analysis confirmed an almost twofold increased risk of AOM in children with A-allele of the HSP70-2 gene (rs1061581) (OR=1.76; OR 95%CI: 1.02-3.05; p=0.026), with the lowest chances in G-allele carriers, especially in the homozygous state (OR=0.50; OR95%CI: 0.25-0.99; p=0.035). Furthermore, the risk of severe AOM increases more than 7 times for purulent discharge from the ear at the age of 7-11 years (OR=7.29; OR 95%CI: 1.44-37.01; p=0.045) with low chances of its development at the pubertal age (12-18 yo) (OR=0.14; OR 95%CI: 0.03-0.70; p=0.019).
Conclusions. The A-allele of the HSP70-2 gene (rs1061581) is associated with an almost twofold increase in AOM risk in the studied pediatric cohort. The risk of severe AOM increases more than sevenfold in cases of purulent ear discharge among children aged 7-11 years
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| ISSN: | 2226-1230 2413-4260 |