Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot study
IntroductionAlthough chronic endometritis (CE) is strongly associated with infertility and adverse pregnancy outcomes, the specific microbiome of women with CE who can conceive remain unclear.MethodsThis study recruited 100 participants aged 18 to 45 years with spontaneously conceived pregnancy who...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1615182/full |
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| author | Hong Gao Hong Gao Na Lu Yahui Chen Genlin Li Huanhuan Li Innie Chen Innie Chen Innie Chen Amanda Black Jenna Gale Jenna Gale Daniel J. Corsi Daniel J. Corsi Daniel J. Corsi Daniel J. Corsi Xiaolan Wang Kristin Connor Shi Wu Wen Shi Wu Wen Shi Wu Wen |
| author_facet | Hong Gao Hong Gao Na Lu Yahui Chen Genlin Li Huanhuan Li Innie Chen Innie Chen Innie Chen Amanda Black Jenna Gale Jenna Gale Daniel J. Corsi Daniel J. Corsi Daniel J. Corsi Daniel J. Corsi Xiaolan Wang Kristin Connor Shi Wu Wen Shi Wu Wen Shi Wu Wen |
| author_sort | Hong Gao |
| collection | DOAJ |
| description | IntroductionAlthough chronic endometritis (CE) is strongly associated with infertility and adverse pregnancy outcomes, the specific microbiome of women with CE who can conceive remain unclear.MethodsThis study recruited 100 participants aged 18 to 45 years with spontaneously conceived pregnancy who opted for pregnancy termination, detected their endometrial microbiome by 16S rRNA, and made a diagnosis of CE.ResultsAmong them, 19 were diagnosed with CE. There was a comparable microbial composition within the endometrium between women with and without CE. The relative abundance of Sphingomonas (21%) and Pseudomonas (8%) were the same in both groups. Compared to women without CE, women with CE exhibited higher abundance of Faecalibacterium (6.5% vs 3.8%), Escherichia-Shigella (3.3% vs 2.6%), Akkermansia (1.65% vs 1.1%), and lower abundance of Lactobacillus (10% vs 14%), and Corynebacterium (1.35% vs 2.15%) at the genus level. Streptococcus, Escherichia-Shigella, Akkermansia and Finegoldia exhibited significant interactions with other microbiome in participants with CE.DiscussionIn women with CE, reproductive potential may be associated with the compositional stability of the endometrial microbiome, whereas an imbalance in the abundance of these microbes may be linked to their pregnancy outcomes. |
| format | Article |
| id | doaj-art-925fbca1e5b0417a828d62007b77c369 |
| institution | DOAJ |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-925fbca1e5b0417a828d62007b77c3692025-08-20T03:05:31ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-08-011510.3389/fcimb.2025.16151821615182Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot studyHong Gao0Hong Gao1Na Lu2Yahui Chen3Genlin Li4Huanhuan Li5Innie Chen6Innie Chen7Innie Chen8Amanda Black9Jenna Gale10Jenna Gale11Daniel J. Corsi12Daniel J. Corsi13Daniel J. Corsi14Daniel J. Corsi15Xiaolan Wang16Kristin Connor17Shi Wu Wen18Shi Wu Wen19Shi Wu Wen20Scientific Research Department, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaOttawa Hospital Research Institute, the Ottawa Hospital, Ottawa, ON, CanadaSchool of Nursing, University of South China, Hengyang, Hunan, ChinaSchool of Nursing, University of South China, Hengyang, Hunan, ChinaCenter for a combination of Obstetrics and Gynecology & Reproductive Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaDepartment of Gynecology, The Second Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaOttawa Hospital Research Institute, the Ottawa Hospital, Ottawa, ON, CanadaDepartment of Obstetrics and Gynecology, University of Ottawa, Ottawa, ON, CanadaSchool of Epidemiology and Public Health, University of Ottawa, Ottawa, ON, CanadaDepartment of Obstetrics and Gynecology, University of Ottawa, Ottawa, ON, CanadaOttawa Hospital Research Institute, the Ottawa Hospital, Ottawa, ON, CanadaDepartment of Obstetrics and Gynecology, University of Ottawa, Ottawa, ON, CanadaOttawa Hospital Research Institute, the Ottawa Hospital, Ottawa, ON, CanadaDepartment of Obstetrics and Gynecology, University of Ottawa, Ottawa, ON, CanadaSchool of Epidemiology and Public Health, University of Ottawa, Ottawa, ON, CanadaSchool of Epidemiology and Public Health, University of Ottawa Faculty of Medicine, Ottawa, ON, CanadaCenter for a combination of Obstetrics and Gynecology & Reproductive Medicine, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaDepartment of Health Sciences, Carleton University, Ottawa, ON, CanadaOttawa Hospital Research Institute, the Ottawa Hospital, Ottawa, ON, CanadaDepartment of Obstetrics and Gynecology, University of Ottawa, Ottawa, ON, CanadaSchool of Epidemiology and Public Health, University of Ottawa, Ottawa, ON, CanadaIntroductionAlthough chronic endometritis (CE) is strongly associated with infertility and adverse pregnancy outcomes, the specific microbiome of women with CE who can conceive remain unclear.MethodsThis study recruited 100 participants aged 18 to 45 years with spontaneously conceived pregnancy who opted for pregnancy termination, detected their endometrial microbiome by 16S rRNA, and made a diagnosis of CE.ResultsAmong them, 19 were diagnosed with CE. There was a comparable microbial composition within the endometrium between women with and without CE. The relative abundance of Sphingomonas (21%) and Pseudomonas (8%) were the same in both groups. Compared to women without CE, women with CE exhibited higher abundance of Faecalibacterium (6.5% vs 3.8%), Escherichia-Shigella (3.3% vs 2.6%), Akkermansia (1.65% vs 1.1%), and lower abundance of Lactobacillus (10% vs 14%), and Corynebacterium (1.35% vs 2.15%) at the genus level. Streptococcus, Escherichia-Shigella, Akkermansia and Finegoldia exhibited significant interactions with other microbiome in participants with CE.DiscussionIn women with CE, reproductive potential may be associated with the compositional stability of the endometrial microbiome, whereas an imbalance in the abundance of these microbes may be linked to their pregnancy outcomes.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1615182/fullendometrial microbiomechronic endometritisearly pregnancy16S rRNAhost factors |
| spellingShingle | Hong Gao Hong Gao Na Lu Yahui Chen Genlin Li Huanhuan Li Innie Chen Innie Chen Innie Chen Amanda Black Jenna Gale Jenna Gale Daniel J. Corsi Daniel J. Corsi Daniel J. Corsi Daniel J. Corsi Xiaolan Wang Kristin Connor Shi Wu Wen Shi Wu Wen Shi Wu Wen Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot study Frontiers in Cellular and Infection Microbiology endometrial microbiome chronic endometritis early pregnancy 16S rRNA host factors |
| title | Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot study |
| title_full | Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot study |
| title_fullStr | Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot study |
| title_full_unstemmed | Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot study |
| title_short | Endometrial microbiome during early pregnancy among women with and without chronic endometritis: a pilot study |
| title_sort | endometrial microbiome during early pregnancy among women with and without chronic endometritis a pilot study |
| topic | endometrial microbiome chronic endometritis early pregnancy 16S rRNA host factors |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1615182/full |
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