The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genes
BackgroundVoghera pepper (VP) extracts were demonstrated to have anti-oxidant ability in several cell types.PurposeThis study aimed to assess whether VP-extracts could lower oxidative stress and modulate thyroid cancer (TC) cells behavior in vitro.MethodsExtracts were analyzed using the LC-DAD-MS sy...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-08-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1574180/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849225266942967808 |
|---|---|
| author | Francesca Coperchini Fabrizio De Luca Alessia Greco Laura Croce Laura Croce Elena Franchi Marsida Teliti Marsida Teliti Patrizia Pignatti Flavia Magri Flavia Magri Maria Grazia Bottone Mario Rotondi Mario Rotondi |
| author_facet | Francesca Coperchini Fabrizio De Luca Alessia Greco Laura Croce Laura Croce Elena Franchi Marsida Teliti Marsida Teliti Patrizia Pignatti Flavia Magri Flavia Magri Maria Grazia Bottone Mario Rotondi Mario Rotondi |
| author_sort | Francesca Coperchini |
| collection | DOAJ |
| description | BackgroundVoghera pepper (VP) extracts were demonstrated to have anti-oxidant ability in several cell types.PurposeThis study aimed to assess whether VP-extracts could lower oxidative stress and modulate thyroid cancer (TC) cells behavior in vitro.MethodsExtracts were analyzed using the LC-DAD-MS system. Thyroid cell lines, both normal (NHT) and cancerous (TPC-1 and 8505C) were treated with increasing concentrations of Yellow (YVP) and Green (GVP) VP-extracts over time. Viability and proliferation were assessed in all cell types. Changes in Reactive-oxygen-species (ROS) production by TPC-1 and 8505C were assessed by flow-cytometry. The mRNA expression of anti-oxidant mediators (NFE2L2, HMOX1, SOD2 and CAT), epithelial-to-mesenchymal transition markers (POU5F1, SNAI1, TWIST1, SNAI2 and VIM) and thyroid-differentiation-related genes (NKX2-1 and PAX8) were evaluated by RT-PCR.ResultsTreatment with GVP or YVP reduced the viability of TPC-1 and 8505C but not those of NHT, without effects on cells proliferation. GVP and YVP reduced basal and H2O2-induced ROS production in TC cells. GVP and YVP up-regulated mRNA levels of several anti-oxidant genes. GVP and YVP reduced mRNA of POU5F1 in TPC-1 and 8505C. Finally, the mRNA of PAX-8 was reduced by GVP and YVP extracts in TPC-1 and 8505C, while NKX2-1 was reduced by both GVP and YVP in TPC-1 and only by GVP exposure in 8505C.ConclusionThis is the first demonstration of the potential beneficial effects of VP extracts in TC in terms of reduction of oxidative stress, increase of antioxidant markers, and modulation of markers of metastasis and de-differentiation in TC cells. |
| format | Article |
| id | doaj-art-92589757e84548808d9831f7614d715e |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj-art-92589757e84548808d9831f7614d715e2025-08-25T05:25:29ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-08-011210.3389/fnut.2025.15741801574180The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genesFrancesca Coperchini0Fabrizio De Luca1Alessia Greco2Laura Croce3Laura Croce4Elena Franchi5Marsida Teliti6Marsida Teliti7Patrizia Pignatti8Flavia Magri9Flavia Magri10Maria Grazia Bottone11Mario Rotondi12Mario Rotondi13Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Pavia, ItalyDepartment of Internal Medicine and Therapeutics, University of Pavia, Pavia, ItalyDepartment of Internal Medicine and Therapeutics, University of Pavia, Pavia, ItalyUnit of Endocrinology and Metabolism, Laboratory for Endocrine Disruptors, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, ItalyDepartment of Internal Medicine and Therapeutics, University of Pavia, Pavia, ItalyDepartment of Internal Medicine and Therapeutics, University of Pavia, Pavia, ItalyUnit of Endocrinology and Metabolism, Laboratory for Endocrine Disruptors, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, ItalyAllergy and Immunology Unit, ICS Maugeri IRCCS, Pavia, ItalyDepartment of Internal Medicine and Therapeutics, University of Pavia, Pavia, ItalyUnit of Endocrinology and Metabolism, Laboratory for Endocrine Disruptors, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, ItalyDepartment of Biology and Biotechnology “L. Spallanzani”, University of Pavia, Pavia, ItalyDepartment of Internal Medicine and Therapeutics, University of Pavia, Pavia, ItalyUnit of Endocrinology and Metabolism, Laboratory for Endocrine Disruptors, Istituti Clinici Scientifici Maugeri IRCCS, Pavia, ItalyBackgroundVoghera pepper (VP) extracts were demonstrated to have anti-oxidant ability in several cell types.PurposeThis study aimed to assess whether VP-extracts could lower oxidative stress and modulate thyroid cancer (TC) cells behavior in vitro.MethodsExtracts were analyzed using the LC-DAD-MS system. Thyroid cell lines, both normal (NHT) and cancerous (TPC-1 and 8505C) were treated with increasing concentrations of Yellow (YVP) and Green (GVP) VP-extracts over time. Viability and proliferation were assessed in all cell types. Changes in Reactive-oxygen-species (ROS) production by TPC-1 and 8505C were assessed by flow-cytometry. The mRNA expression of anti-oxidant mediators (NFE2L2, HMOX1, SOD2 and CAT), epithelial-to-mesenchymal transition markers (POU5F1, SNAI1, TWIST1, SNAI2 and VIM) and thyroid-differentiation-related genes (NKX2-1 and PAX8) were evaluated by RT-PCR.ResultsTreatment with GVP or YVP reduced the viability of TPC-1 and 8505C but not those of NHT, without effects on cells proliferation. GVP and YVP reduced basal and H2O2-induced ROS production in TC cells. GVP and YVP up-regulated mRNA levels of several anti-oxidant genes. GVP and YVP reduced mRNA of POU5F1 in TPC-1 and 8505C. Finally, the mRNA of PAX-8 was reduced by GVP and YVP extracts in TPC-1 and 8505C, while NKX2-1 was reduced by both GVP and YVP in TPC-1 and only by GVP exposure in 8505C.ConclusionThis is the first demonstration of the potential beneficial effects of VP extracts in TC in terms of reduction of oxidative stress, increase of antioxidant markers, and modulation of markers of metastasis and de-differentiation in TC cells.https://www.frontiersin.org/articles/10.3389/fnut.2025.1574180/fullVoghera pepperthyroid canceroxidative stressphytochemicalROSanti-oxidant genes |
| spellingShingle | Francesca Coperchini Fabrizio De Luca Alessia Greco Laura Croce Laura Croce Elena Franchi Marsida Teliti Marsida Teliti Patrizia Pignatti Flavia Magri Flavia Magri Maria Grazia Bottone Mario Rotondi Mario Rotondi The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genes Frontiers in Nutrition Voghera pepper thyroid cancer oxidative stress phytochemical ROS anti-oxidant genes |
| title | The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genes |
| title_full | The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genes |
| title_fullStr | The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genes |
| title_full_unstemmed | The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genes |
| title_short | The in vitro effects of Voghera sweet pepper on thyroid cancer cells: modulation of oxidative stress and pro-tumorigenic genes |
| title_sort | in vitro effects of voghera sweet pepper on thyroid cancer cells modulation of oxidative stress and pro tumorigenic genes |
| topic | Voghera pepper thyroid cancer oxidative stress phytochemical ROS anti-oxidant genes |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1574180/full |
| work_keys_str_mv | AT francescacoperchini theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT fabriziodeluca theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT alessiagreco theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT lauracroce theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT lauracroce theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT elenafranchi theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT marsidateliti theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT marsidateliti theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT patriziapignatti theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT flaviamagri theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT flaviamagri theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT mariagraziabottone theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT mariorotondi theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT mariorotondi theinvitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT francescacoperchini invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT fabriziodeluca invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT alessiagreco invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT lauracroce invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT lauracroce invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT elenafranchi invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT marsidateliti invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT marsidateliti invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT patriziapignatti invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT flaviamagri invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT flaviamagri invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT mariagraziabottone invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT mariorotondi invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes AT mariorotondi invitroeffectsofvogherasweetpepperonthyroidcancercellsmodulationofoxidativestressandprotumorigenicgenes |