Quantitative bead–based multiplex assay for simultaneous determination of IgG concentrations of pertussis toxin, filamentous hemagglutinin, pertactin, diphtheria, tetanus, Haemophilus influenzae b, and hepatitis B in human serum samples
BackgroundMultiplex serological assays provide opportunities for seroprevalence studies and for evaluating antibodies post-vaccination. In this report, we describe the development and validation of a seven-plex bead-based assay for quantifying human immunoglobulin G (IgG) antibodies against pertussi...
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| Main Authors: | Vishal Rathod, Sagar Katke, Sumant Patil, Sachin Bhandare, Laxmikant Kadam, Manish Gautam, Prabhu Gumma, Krishna Manoj Kumar, Laura Hassall, Cathy Asokanathan, Alex Douglas-Bardsley, Kevin Markey, Sumit Gupta, Harish Rao, Sameer Parekh, Pramod Pujari, Hitt Sharma, Umesh Shaligram, Sunil Gairola |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1587567/full |
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