Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy
Brillouin microscopy has been widely used in the mechanical imaging of cells and tissues, and the signal-to-noise (SNR) ratio limits the spectral integration time. Impulsive stimulated Brillouin scattering (ISBS) microscopy is a new elastic imaging technique. As a variant of stimulated Brillouin sca...
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| Language: | English |
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Elsevier
2025-04-01
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| Series: | Photoacoustics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597925000151 |
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| author | Taoran Le Jiarui Li Haoyun Wei Yan Li |
| author_facet | Taoran Le Jiarui Li Haoyun Wei Yan Li |
| author_sort | Taoran Le |
| collection | DOAJ |
| description | Brillouin microscopy has been widely used in the mechanical imaging of cells and tissues, and the signal-to-noise (SNR) ratio limits the spectral integration time. Impulsive stimulated Brillouin scattering (ISBS) microscopy is a new elastic imaging technique. As a variant of stimulated Brillouin scattering (SBS), ISBS can overcome the weak signal of spontaneous Brillouin scattering. A simple model can estimate SBS gain. However, the theoretical ISBS gain has not been compared with SBS gain. This paper gives the theoretical ISBS gain estimation, and experiments are designed to verify estimation reliability. The heterodyne ISBS gain coefficient can be much higher than SBS gain coefficient. The relationship between ISBS gain coefficient and spatial resolution is then discussed. We anticipate that the ISBS setup optimization can improve spatial resolution and gain, potentially enabling fast and high spatial resolution imaging of biological cells. |
| format | Article |
| id | doaj-art-92457af9b7b14868b802c62340b3fbe7 |
| institution | OA Journals |
| issn | 2213-5979 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj-art-92457af9b7b14868b802c62340b3fbe72025-08-20T02:00:59ZengElsevierPhotoacoustics2213-59792025-04-014210069610.1016/j.pacs.2025.100696Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopyTaoran Le0Jiarui Li1Haoyun Wei2Yan Li3State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, ChinaState Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, ChinaState Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, ChinaCorresponding author.; State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100084, ChinaBrillouin microscopy has been widely used in the mechanical imaging of cells and tissues, and the signal-to-noise (SNR) ratio limits the spectral integration time. Impulsive stimulated Brillouin scattering (ISBS) microscopy is a new elastic imaging technique. As a variant of stimulated Brillouin scattering (SBS), ISBS can overcome the weak signal of spontaneous Brillouin scattering. A simple model can estimate SBS gain. However, the theoretical ISBS gain has not been compared with SBS gain. This paper gives the theoretical ISBS gain estimation, and experiments are designed to verify estimation reliability. The heterodyne ISBS gain coefficient can be much higher than SBS gain coefficient. The relationship between ISBS gain coefficient and spatial resolution is then discussed. We anticipate that the ISBS setup optimization can improve spatial resolution and gain, potentially enabling fast and high spatial resolution imaging of biological cells.http://www.sciencedirect.com/science/article/pii/S2213597925000151Impulsive stimulated Brillouin scatteringBrillouin microscopyBrillouin gain |
| spellingShingle | Taoran Le Jiarui Li Haoyun Wei Yan Li Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy Photoacoustics Impulsive stimulated Brillouin scattering Brillouin microscopy Brillouin gain |
| title | Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy |
| title_full | Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy |
| title_fullStr | Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy |
| title_full_unstemmed | Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy |
| title_short | Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy |
| title_sort | improvement of gain and spatial resolution for impulsive stimulated brillouin scattering microscopy |
| topic | Impulsive stimulated Brillouin scattering Brillouin microscopy Brillouin gain |
| url | http://www.sciencedirect.com/science/article/pii/S2213597925000151 |
| work_keys_str_mv | AT taoranle improvementofgainandspatialresolutionforimpulsivestimulatedbrillouinscatteringmicroscopy AT jiaruili improvementofgainandspatialresolutionforimpulsivestimulatedbrillouinscatteringmicroscopy AT haoyunwei improvementofgainandspatialresolutionforimpulsivestimulatedbrillouinscatteringmicroscopy AT yanli improvementofgainandspatialresolutionforimpulsivestimulatedbrillouinscatteringmicroscopy |