Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast China

This research targets straw return in Farm 852’s albic soil, China. The soil is nutrient-poor with few microbes and slow straw decomposition. Through fixed-point sampling and bacterial screening, an actinomycete consortium consisting of four strains was assembled, and two of them were identified as...

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Main Authors: Xiujie Gong, Yang Yu, Guoyi Lv, Yubo Hao, Lingli Wang, Juntao Ma, Yubo Jiang, Jiahe Zou, Jingyang Li, Qiuju Wang
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/2/385
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author Xiujie Gong
Yang Yu
Guoyi Lv
Yubo Hao
Lingli Wang
Juntao Ma
Yubo Jiang
Jiahe Zou
Jingyang Li
Qiuju Wang
author_facet Xiujie Gong
Yang Yu
Guoyi Lv
Yubo Hao
Lingli Wang
Juntao Ma
Yubo Jiang
Jiahe Zou
Jingyang Li
Qiuju Wang
author_sort Xiujie Gong
collection DOAJ
description This research targets straw return in Farm 852’s albic soil, China. The soil is nutrient-poor with few microbes and slow straw decomposition. Through fixed-point sampling and bacterial screening, an actinomycete consortium consisting of four strains was assembled, and two of them were identified as new actinomycetes. After 7 days of fermentation, the lignocellulose degradation rates of this consortium outstripped those of single strains, with cellulose degraded at 69.07%, hemicellulose at 64.98%, and lignin at 68.95%. FTIR, XRD, and SEM verified the damage inflicted on the straw structure. Lab simulations found group D (with the consortium) had a higher straw weight loss rate than group C (with commercialized microbial agents) and controls. The compound actinomycetes stepped up the bacterial abundance with the passage of time. In contrast, their effect on fungal abundance was hardly noticeable, but they had markedly ameliorated the soil fertility. These findings prove that the microbial consortium effectively accelerates straw decomposition and boosts soil microbe abundance and fertility in albic soil. It shows great potential for straw return and provides a microbial solution for this field.
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publisher MDPI AG
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series Microorganisms
spelling doaj-art-923849ee798345779066e2abd2cb4c6d2025-08-20T02:03:25ZengMDPI AGMicroorganisms2076-26072025-02-0113238510.3390/microorganisms13020385Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast ChinaXiujie Gong0Yang Yu1Guoyi Lv2Yubo Hao3Lingli Wang4Juntao Ma5Yubo Jiang6Jiahe Zou7Jingyang Li8Qiuju Wang9Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaShenyang Institute of Applied Ecology, Chinese Academy of Sciences, No. 72 Wenhua Road, Shenhe District, Shenyang 110016, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaHeilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin 150086, ChinaThis research targets straw return in Farm 852’s albic soil, China. The soil is nutrient-poor with few microbes and slow straw decomposition. Through fixed-point sampling and bacterial screening, an actinomycete consortium consisting of four strains was assembled, and two of them were identified as new actinomycetes. After 7 days of fermentation, the lignocellulose degradation rates of this consortium outstripped those of single strains, with cellulose degraded at 69.07%, hemicellulose at 64.98%, and lignin at 68.95%. FTIR, XRD, and SEM verified the damage inflicted on the straw structure. Lab simulations found group D (with the consortium) had a higher straw weight loss rate than group C (with commercialized microbial agents) and controls. The compound actinomycetes stepped up the bacterial abundance with the passage of time. In contrast, their effect on fungal abundance was hardly noticeable, but they had markedly ameliorated the soil fertility. These findings prove that the microbial consortium effectively accelerates straw decomposition and boosts soil microbe abundance and fertility in albic soil. It shows great potential for straw return and provides a microbial solution for this field.https://www.mdpi.com/2076-2607/13/2/385albic soilstraw returnbacteria screeningactinomycetesstraw degradation
spellingShingle Xiujie Gong
Yang Yu
Guoyi Lv
Yubo Hao
Lingli Wang
Juntao Ma
Yubo Jiang
Jiahe Zou
Jingyang Li
Qiuju Wang
Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast China
Microorganisms
albic soil
straw return
bacteria screening
actinomycetes
straw degradation
title Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast China
title_full Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast China
title_fullStr Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast China
title_full_unstemmed Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast China
title_short Construction and Effect Analysis of a Mixed <i>Actinomycete</i> Flora for Straw Returning to Albic Soil in Northeast China
title_sort construction and effect analysis of a mixed i actinomycete i flora for straw returning to albic soil in northeast china
topic albic soil
straw return
bacteria screening
actinomycetes
straw degradation
url https://www.mdpi.com/2076-2607/13/2/385
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