Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells

Isolation and Time Lapse Microscopy of Highly Pure Hepatic Stellate Cells

Hepatic stellate cells (HSC) are the main effector cells for liver fibrosis. We aimed at optimizing HSC isolation by an additional step of fluorescence-activated cell sorting (FACS) via a UV laser. HSC were isolated from livers of healthy mice and animals subjected to experimental fibrosis. HSC isol...

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Bibliographic Details
Main Authors: Matthias Bartneck, Klaudia Theresa Warzecha, Carmen Gabriele Tag, Sibille Sauer-Lehnen, Felix Heymann, Christian Trautwein, Ralf Weiskirchen, Frank Tacke
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2015/417023
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