Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i>
<i>Fallopia multiflora</i>, a perennial herb in the <i>Polygonaceae</i> family belonging to the genus <i>Fallopia</i> Adanson, is traditionally used as a Chinese herbal medicine. However, there is still confusion about the botanical origin of the species and the p...
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2024-12-01
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| author | Raveendar Sebastin Ki Hyun Kim Hye Ran Shin Jin-Tae Jeong Ju-Kyung Yu Yoon-Sup So Jong-Wook Chung |
| author_facet | Raveendar Sebastin Ki Hyun Kim Hye Ran Shin Jin-Tae Jeong Ju-Kyung Yu Yoon-Sup So Jong-Wook Chung |
| author_sort | Raveendar Sebastin |
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| description | <i>Fallopia multiflora</i>, a perennial herb in the <i>Polygonaceae</i> family belonging to the genus <i>Fallopia</i> Adanson, is traditionally used as a Chinese herbal medicine. However, there is still confusion about the botanical origin of the species and the phylogenetic relationship between the cultivars and the wild relatives. To develop an efficient identification method, a molecular analysis was performed using SSR markers. The genetic diversity of the <i>F. multiflora</i> genetic resources has been assessed by using 10 locally collected accessions, including varieties and landraces. We screened 100 pairs of SSR primers and selected 71 successfully amplified SSR markers, in which one SSR was found to be a monomorphic marker. The results indicated that the number of alleles (NA) ranged from 2 to 10, with an average of 4.1 alleles. The major allele frequency (MAF) spanned from 0.20 to 0.90, the observed heterozygosity (HO) ranged from 0 to 0.80, and the polymorphic information content (PIC) varied between 0.16 and 0.86. Clustering analysis using an unweighted pair group mean algorithm (UPGMA) with all 70 SSR markers revealed three clusters among the <i>F. multiflora</i> accessions. Furthermore, seven minimum marker set combinations were identified and proved useful for variety identification. Therefore, these SSR markers could be valuable for various applications, including cultivar identification and assessing the purity of <i>F. multiflora</i> populations. Three genetic groups of <i>F. multiflora</i> should be considered as independent units for conservation and germplasm management of the species. |
| format | Article |
| id | doaj-art-92135957b2e3430ca18a4c03ee001957 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-92135957b2e3430ca18a4c03ee0019572025-01-24T13:34:25ZengMDPI AGHorticulturae2311-75242024-12-01111210.3390/horticulturae11010002Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i>Raveendar Sebastin0Ki Hyun Kim1Hye Ran Shin2Jin-Tae Jeong3Ju-Kyung Yu4Yoon-Sup So5Jong-Wook Chung6Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Republic of KoreaChungbuk Agricultural Research and Extension Services, Cheongju 28130, Republic of KoreaDepartment of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Herbal Crop Research, NIHHS, R.D.A., Eumseong 27709, Republic of KoreaDepartment of Crop Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Crop Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Industrial Plant Science and Technology, Chungbuk National University, Cheongju 28644, Republic of Korea<i>Fallopia multiflora</i>, a perennial herb in the <i>Polygonaceae</i> family belonging to the genus <i>Fallopia</i> Adanson, is traditionally used as a Chinese herbal medicine. However, there is still confusion about the botanical origin of the species and the phylogenetic relationship between the cultivars and the wild relatives. To develop an efficient identification method, a molecular analysis was performed using SSR markers. The genetic diversity of the <i>F. multiflora</i> genetic resources has been assessed by using 10 locally collected accessions, including varieties and landraces. We screened 100 pairs of SSR primers and selected 71 successfully amplified SSR markers, in which one SSR was found to be a monomorphic marker. The results indicated that the number of alleles (NA) ranged from 2 to 10, with an average of 4.1 alleles. The major allele frequency (MAF) spanned from 0.20 to 0.90, the observed heterozygosity (HO) ranged from 0 to 0.80, and the polymorphic information content (PIC) varied between 0.16 and 0.86. Clustering analysis using an unweighted pair group mean algorithm (UPGMA) with all 70 SSR markers revealed three clusters among the <i>F. multiflora</i> accessions. Furthermore, seven minimum marker set combinations were identified and proved useful for variety identification. Therefore, these SSR markers could be valuable for various applications, including cultivar identification and assessing the purity of <i>F. multiflora</i> populations. Three genetic groups of <i>F. multiflora</i> should be considered as independent units for conservation and germplasm management of the species.https://www.mdpi.com/2311-7524/11/1/2<i>Fallopia</i>genetic diversitySSRpolymorphismmarker development |
| spellingShingle | Raveendar Sebastin Ki Hyun Kim Hye Ran Shin Jin-Tae Jeong Ju-Kyung Yu Yoon-Sup So Jong-Wook Chung Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i> Horticulturae <i>Fallopia</i> genetic diversity SSR polymorphism marker development |
| title | Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i> |
| title_full | Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i> |
| title_fullStr | Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i> |
| title_full_unstemmed | Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i> |
| title_short | Development of Genomic SSR Markers for Assessing Genetic Diversity in Korean Native <i>Fallopia multiflora</i> |
| title_sort | development of genomic ssr markers for assessing genetic diversity in korean native i fallopia multiflora i |
| topic | <i>Fallopia</i> genetic diversity SSR polymorphism marker development |
| url | https://www.mdpi.com/2311-7524/11/1/2 |
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