Quantitative Super‐Resolution Imaging of Molecular Tension
Abstract DNA‐based molecular tension probes have revolutionized the localization of mechanical events in live cells with super‐resolution. However, imaging the magnitude of these forces at super‐resolution has been challenging. Here, qtPAINT (quantitative tension points accumulation for imaging in n...
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| Format: | Article |
| Language: | English |
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202408280 |
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| author | Seong Ho Kim Adam B. Yasunaga Hongyuan Zhang Kevin D. Whitley Isaac T. S. Li |
| author_facet | Seong Ho Kim Adam B. Yasunaga Hongyuan Zhang Kevin D. Whitley Isaac T. S. Li |
| author_sort | Seong Ho Kim |
| collection | DOAJ |
| description | Abstract DNA‐based molecular tension probes have revolutionized the localization of mechanical events in live cells with super‐resolution. However, imaging the magnitude of these forces at super‐resolution has been challenging. Here, qtPAINT (quantitative tension points accumulation for imaging in nanoscale topography) is introduced as a strategy to image the magnitude of molecular tension with super‐resolution accuracy. By leveraging the force‐dependent dissociation kinetics of short DNA oligonucleotides on their complementary strands, tension is encoded on individual molecules through their binding kinetics. This method allowed for a quantitative analysis of these kinetics, providing a detailed reconstruction of the force magnitudes acting on each tension probe. The technique integrates a molecular‐beacon PAINT imager with a hairpin molecular tension probe, achieving a force quantification range of 9–30 pN and maintaining a spatial resolution of 30–120 nm in low and high‐density regions. Additionally, qtPAINT offers a temporal resolution on the order of a minute, enhancing its applicability for studying dynamic cellular processes. |
| format | Article |
| id | doaj-art-92092d2d53b44482bd2ba6c5ce8a50a7 |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-92092d2d53b44482bd2ba6c5ce8a50a72025-08-20T03:15:34ZengWileyAdvanced Science2198-38442025-07-011228n/an/a10.1002/advs.202408280Quantitative Super‐Resolution Imaging of Molecular TensionSeong Ho Kim0Adam B. Yasunaga1Hongyuan Zhang2Kevin D. Whitley3Isaac T. S. Li4Department of Chemistry University of British Columbia Kelowna BC V1V 1V7 CanadaDepartment of Chemistry University of British Columbia Kelowna BC V1V 1V7 CanadaDepartment of Chemistry University of British Columbia Kelowna BC V1V 1V7 CanadaCentre for Bacterial Cell Biology Biosciences Institute Newcastle University Newcastle upon Tyne NE1 7RU UKDepartment of Chemistry University of British Columbia Kelowna BC V1V 1V7 CanadaAbstract DNA‐based molecular tension probes have revolutionized the localization of mechanical events in live cells with super‐resolution. However, imaging the magnitude of these forces at super‐resolution has been challenging. Here, qtPAINT (quantitative tension points accumulation for imaging in nanoscale topography) is introduced as a strategy to image the magnitude of molecular tension with super‐resolution accuracy. By leveraging the force‐dependent dissociation kinetics of short DNA oligonucleotides on their complementary strands, tension is encoded on individual molecules through their binding kinetics. This method allowed for a quantitative analysis of these kinetics, providing a detailed reconstruction of the force magnitudes acting on each tension probe. The technique integrates a molecular‐beacon PAINT imager with a hairpin molecular tension probe, achieving a force quantification range of 9–30 pN and maintaining a spatial resolution of 30–120 nm in low and high‐density regions. Additionally, qtPAINT offers a temporal resolution on the order of a minute, enhancing its applicability for studying dynamic cellular processes.https://doi.org/10.1002/advs.202408280DNA‐PAINTfunctional super‐resolution imagingmolecular beaconmolecular tension sensor |
| spellingShingle | Seong Ho Kim Adam B. Yasunaga Hongyuan Zhang Kevin D. Whitley Isaac T. S. Li Quantitative Super‐Resolution Imaging of Molecular Tension Advanced Science DNA‐PAINT functional super‐resolution imaging molecular beacon molecular tension sensor |
| title | Quantitative Super‐Resolution Imaging of Molecular Tension |
| title_full | Quantitative Super‐Resolution Imaging of Molecular Tension |
| title_fullStr | Quantitative Super‐Resolution Imaging of Molecular Tension |
| title_full_unstemmed | Quantitative Super‐Resolution Imaging of Molecular Tension |
| title_short | Quantitative Super‐Resolution Imaging of Molecular Tension |
| title_sort | quantitative super resolution imaging of molecular tension |
| topic | DNA‐PAINT functional super‐resolution imaging molecular beacon molecular tension sensor |
| url | https://doi.org/10.1002/advs.202408280 |
| work_keys_str_mv | AT seonghokim quantitativesuperresolutionimagingofmoleculartension AT adambyasunaga quantitativesuperresolutionimagingofmoleculartension AT hongyuanzhang quantitativesuperresolutionimagingofmoleculartension AT kevindwhitley quantitativesuperresolutionimagingofmoleculartension AT isaactsli quantitativesuperresolutionimagingofmoleculartension |