Integrative Analyses of Genes Associated with Hashimoto’s Thyroiditis
Objective. Hashimoto’s thyroiditis, also known as chronic lymphocytic thyroiditis, is a common autoimmune thyroiditis, which mostly occurs in young and middle-aged women. It can be manifested as hyperthyroidism in the early stage; hypothyroidism may appear with the progression of the disease. Studie...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/8263829 |
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| author | Kangli Qiu Kai Li Tianshu Zeng Yunfei Liao Jie Min Nan Zhang Miaomiao Peng Wen Kong Lu-lu Chen |
| author_facet | Kangli Qiu Kai Li Tianshu Zeng Yunfei Liao Jie Min Nan Zhang Miaomiao Peng Wen Kong Lu-lu Chen |
| author_sort | Kangli Qiu |
| collection | DOAJ |
| description | Objective. Hashimoto’s thyroiditis, also known as chronic lymphocytic thyroiditis, is a common autoimmune thyroiditis, which mostly occurs in young and middle-aged women. It can be manifested as hyperthyroidism in the early stage; hypothyroidism may appear with the progression of the disease. Studies have shown that multiple factors such as heredity, environment, and autoimmunity are involved in the pathogenesis, but the specific mechanism is not clear. In our study, we tried to find key genes and potential molecular mechanisms of Hashimoto’s thyroiditis to provide new ideas for the therapeutic targets of Hashimoto’s thyroiditis. Method. GSE138198 and GSE54958 were downloaded from the GEO database, and two datasets were combined for analysis. The combined data were normalized to identify the differentially expressed genes (DEGs), and GO and KEGG enrichment analyses were performed. Protein-protein interaction (PPI) networks and hub genes between DEGs were identified. We also used the miRWalk database to identify regulatory miRNAs associated with expressions of DEGs. Result. We identified 182 DEGs (160 upregulated and 22 downregulated) between Hashimoto’s disease patients and the healthy control group. GO analysis showed that DEGs were mostly concentrated in detection of chemical stimulus involved in sensory perception, intermediate filament cytoskeleton, and olfactory receptor activity. KEGG pathway analysis showed that DEGs were mainly related to olfactory transduction. Some members of the KRTAP family and HTR5A, KNG1, DRD3, HTR1D, TAS2R16, INSL5, TAS2R42, and GRM7 are the most important hub genes in the PPI network. In addition, we recognized that OTUD4, LLPH, and ECHDC1 were the most important hub genes in the miRNA-target gene network. Conclusion. In this study, a series of bioinformatics analyses of DEGs were performed to identify the key genes and pathways associated with Hashimoto’s thyroiditis. These genes and pathways provide a more detailed understanding of the pathogenesis of Hashimoto’s disease and provide new ideas for the therapeutic targets of Hashimoto’s thyroiditis. |
| format | Article |
| id | doaj-art-9204512a6b8b432baef5caac354f6a24 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9204512a6b8b432baef5caac354f6a242025-08-20T03:37:08ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/82638298263829Integrative Analyses of Genes Associated with Hashimoto’s ThyroiditisKangli Qiu0Kai Li1Tianshu Zeng2Yunfei Liao3Jie Min4Nan Zhang5Miaomiao Peng6Wen Kong7Lu-lu Chen8Department of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaNetwork and Computing Center, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaDepartment of Endocrinology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, ChinaObjective. Hashimoto’s thyroiditis, also known as chronic lymphocytic thyroiditis, is a common autoimmune thyroiditis, which mostly occurs in young and middle-aged women. It can be manifested as hyperthyroidism in the early stage; hypothyroidism may appear with the progression of the disease. Studies have shown that multiple factors such as heredity, environment, and autoimmunity are involved in the pathogenesis, but the specific mechanism is not clear. In our study, we tried to find key genes and potential molecular mechanisms of Hashimoto’s thyroiditis to provide new ideas for the therapeutic targets of Hashimoto’s thyroiditis. Method. GSE138198 and GSE54958 were downloaded from the GEO database, and two datasets were combined for analysis. The combined data were normalized to identify the differentially expressed genes (DEGs), and GO and KEGG enrichment analyses were performed. Protein-protein interaction (PPI) networks and hub genes between DEGs were identified. We also used the miRWalk database to identify regulatory miRNAs associated with expressions of DEGs. Result. We identified 182 DEGs (160 upregulated and 22 downregulated) between Hashimoto’s disease patients and the healthy control group. GO analysis showed that DEGs were mostly concentrated in detection of chemical stimulus involved in sensory perception, intermediate filament cytoskeleton, and olfactory receptor activity. KEGG pathway analysis showed that DEGs were mainly related to olfactory transduction. Some members of the KRTAP family and HTR5A, KNG1, DRD3, HTR1D, TAS2R16, INSL5, TAS2R42, and GRM7 are the most important hub genes in the PPI network. In addition, we recognized that OTUD4, LLPH, and ECHDC1 were the most important hub genes in the miRNA-target gene network. Conclusion. In this study, a series of bioinformatics analyses of DEGs were performed to identify the key genes and pathways associated with Hashimoto’s thyroiditis. These genes and pathways provide a more detailed understanding of the pathogenesis of Hashimoto’s disease and provide new ideas for the therapeutic targets of Hashimoto’s thyroiditis.http://dx.doi.org/10.1155/2021/8263829 |
| spellingShingle | Kangli Qiu Kai Li Tianshu Zeng Yunfei Liao Jie Min Nan Zhang Miaomiao Peng Wen Kong Lu-lu Chen Integrative Analyses of Genes Associated with Hashimoto’s Thyroiditis Journal of Immunology Research |
| title | Integrative Analyses of Genes Associated with Hashimoto’s Thyroiditis |
| title_full | Integrative Analyses of Genes Associated with Hashimoto’s Thyroiditis |
| title_fullStr | Integrative Analyses of Genes Associated with Hashimoto’s Thyroiditis |
| title_full_unstemmed | Integrative Analyses of Genes Associated with Hashimoto’s Thyroiditis |
| title_short | Integrative Analyses of Genes Associated with Hashimoto’s Thyroiditis |
| title_sort | integrative analyses of genes associated with hashimoto s thyroiditis |
| url | http://dx.doi.org/10.1155/2021/8263829 |
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