Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin
Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity emitted by epidermal and dermal fluorophores...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Dermatology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2012/810749 |
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| _version_ | 1832567389377527808 |
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| author | Stefania Seidenari Federica Arginelli Sara Bassoli Jennifer Cautela Paul M. W. French Mario Guanti Davide Guardoli Karsten König Clifford Talbot Chris Dunsby |
| author_facet | Stefania Seidenari Federica Arginelli Sara Bassoli Jennifer Cautela Paul M. W. French Mario Guanti Davide Guardoli Karsten König Clifford Talbot Chris Dunsby |
| author_sort | Stefania Seidenari |
| collection | DOAJ |
| description | Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity emitted by epidermal and dermal fluorophores and by the extra-cellular matrix, fluorescence lifetime imaging (FLIM), is generated by the fluorescence decay rate. This innovative technique can be applied to the study of living skin, cell cultures and ex vivo samples. Although still limited to the clinical research field, the development of multiphoton laser microscopy is thought to become suitable for a practical application in the next few years: in this paper, we performed an accurate review of the studies published so far, considering the possible fields of application of this imaging method and providing high quality images acquired in the Department of Dermatology of the University of Modena. |
| format | Article |
| id | doaj-art-920223aff04744068361219227e6c36e |
| institution | Kabale University |
| issn | 1687-6105 1687-6113 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Dermatology Research and Practice |
| spelling | doaj-art-920223aff04744068361219227e6c36e2025-02-03T01:01:38ZengWileyDermatology Research and Practice1687-61051687-61132012-01-01201210.1155/2012/810749810749Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the SkinStefania Seidenari0Federica Arginelli1Sara Bassoli2Jennifer Cautela3Paul M. W. French4Mario Guanti5Davide Guardoli6Karsten König7Clifford Talbot8Chris Dunsby9Department of Dermatology, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Dermatology, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Dermatology, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Dermatology, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Physics, Imperial College London, South Kensington Campus, London SW7 2AZ, UKDepartment of Dermatology, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Dermatology, University of Modena and Reggio Emilia, 41124 Modena, ItalyDepartment of Biophotonics and Lasertechnology, Saarland University, Campus A5.1, 66123 Saarbrücken, GermanyDepartment of Physics, Imperial College London, South Kensington Campus, London SW7 2AZ, UKDepartment of Physics, Imperial College London, South Kensington Campus, London SW7 2AZ, UKMultiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which is based both on autofluorescence and fluorescence lifetime imaging. Whereas the former considers fluorescence intensity emitted by epidermal and dermal fluorophores and by the extra-cellular matrix, fluorescence lifetime imaging (FLIM), is generated by the fluorescence decay rate. This innovative technique can be applied to the study of living skin, cell cultures and ex vivo samples. Although still limited to the clinical research field, the development of multiphoton laser microscopy is thought to become suitable for a practical application in the next few years: in this paper, we performed an accurate review of the studies published so far, considering the possible fields of application of this imaging method and providing high quality images acquired in the Department of Dermatology of the University of Modena.http://dx.doi.org/10.1155/2012/810749 |
| spellingShingle | Stefania Seidenari Federica Arginelli Sara Bassoli Jennifer Cautela Paul M. W. French Mario Guanti Davide Guardoli Karsten König Clifford Talbot Chris Dunsby Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin Dermatology Research and Practice |
| title | Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin |
| title_full | Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin |
| title_fullStr | Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin |
| title_full_unstemmed | Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin |
| title_short | Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin |
| title_sort | multiphoton laser microscopy and fluorescence lifetime imaging for the evaluation of the skin |
| url | http://dx.doi.org/10.1155/2012/810749 |
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