Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MS
Caine-based anesthetics are frequently used in the production and transportation of aquatic products, but residues in fish threaten human health. A rapid, sensitive, and effective method was developed for detecting caine-based anesthetics and their metabolite residues in fish using ultra-high perfor...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524009209 |
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| author | Shouer Lin Wenqian Qiu Yongyou Hua Yan Yang |
| author_facet | Shouer Lin Wenqian Qiu Yongyou Hua Yan Yang |
| author_sort | Shouer Lin |
| collection | DOAJ |
| description | Caine-based anesthetics are frequently used in the production and transportation of aquatic products, but residues in fish threaten human health. A rapid, sensitive, and effective method was developed for detecting caine-based anesthetics and their metabolite residues in fish using ultra-high performance liquid chromatography-tandem mass spectrometry coupled with a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. The sample was extracted with 0.05 % (v/v) formic acid-80 % acetonitrile, 4 g NaCl was added for liquid partitioning, and 50 mg C18 was used for purification. Matrix-matched calibrations showed good correlation coefficients with R2 ≥ 0.9942. The limits of detection were 0.5–4.4 ng/g. The recoveries ranged from 71.4 %–115.8 %, and the relative standard deviation for intra-day and inter-day precision was less than 8.5 % and 9.2 %, respectively. This method effectively analyzed the residues of caine-based anesthetics and their metabolites in fish and could be applied to aquatic product anesthetic regulation. |
| format | Article |
| id | doaj-art-920107ca5e0846faa4b015ae03cbb9ce |
| institution | OA Journals |
| issn | 2590-1575 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-920107ca5e0846faa4b015ae03cbb9ce2025-08-20T01:59:31ZengElsevierFood Chemistry: X2590-15752024-12-012410203210.1016/j.fochx.2024.102032Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MSShouer Lin0Wenqian Qiu1Yongyou Hua2Yan Yang3Physical and Chemical Analysis Department, Fujian Provincial Center For Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou, Fujian 350001, ChinaPhysical and Chemical Analysis Department, Fujian Provincial Center For Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou, Fujian 350001, ChinaPhysical and Chemical Analysis Department, Fujian Provincial Center For Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou, Fujian 350001, ChinaCorresponding author at: No. 386 Chongan Road, Fujian Provincial Center For Disease Control and Prevention, Fuzhou, Fujian 350012, China.; Physical and Chemical Analysis Department, Fujian Provincial Center For Disease Control and Prevention, Fujian Provincial Key Laboratory of Zoonosis Research, Fuzhou, Fujian 350001, ChinaCaine-based anesthetics are frequently used in the production and transportation of aquatic products, but residues in fish threaten human health. A rapid, sensitive, and effective method was developed for detecting caine-based anesthetics and their metabolite residues in fish using ultra-high performance liquid chromatography-tandem mass spectrometry coupled with a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method. The sample was extracted with 0.05 % (v/v) formic acid-80 % acetonitrile, 4 g NaCl was added for liquid partitioning, and 50 mg C18 was used for purification. Matrix-matched calibrations showed good correlation coefficients with R2 ≥ 0.9942. The limits of detection were 0.5–4.4 ng/g. The recoveries ranged from 71.4 %–115.8 %, and the relative standard deviation for intra-day and inter-day precision was less than 8.5 % and 9.2 %, respectively. This method effectively analyzed the residues of caine-based anesthetics and their metabolites in fish and could be applied to aquatic product anesthetic regulation.http://www.sciencedirect.com/science/article/pii/S2590157524009209Caine-based anestheticsMetabolitesResiduesFishUPLC-MS/MSQuEChERS |
| spellingShingle | Shouer Lin Wenqian Qiu Yongyou Hua Yan Yang Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MS Food Chemistry: X Caine-based anesthetics Metabolites Residues Fish UPLC-MS/MS QuEChERS |
| title | Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MS |
| title_full | Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MS |
| title_fullStr | Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MS |
| title_full_unstemmed | Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MS |
| title_short | Rapid determination of caine-based anesthetics and their metabolite residues in fish using a modified QuEChERS method coupled with UPLC-MS/MS |
| title_sort | rapid determination of caine based anesthetics and their metabolite residues in fish using a modified quechers method coupled with uplc ms ms |
| topic | Caine-based anesthetics Metabolites Residues Fish UPLC-MS/MS QuEChERS |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524009209 |
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