Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients
Background. Altered lipid profiles have consistently been linked to cerebrovascular events. Ischemic stroke (IS) was a common comorbid condition established in type 2 diabetes mellitus (T2DM). The apolipoprotein E (ApoE) gene which has a notably critical function in lipoprotein metabolism is believe...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2021/5527736 |
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| author | Ni Putu Tesi Maratni Made Ratna Saraswati Ni Nyoman Ayu Dewi I Wayan Putu Sutirta Yasa I Putu Eka Widyadharma Ida Bagus Kusuma Putra Ketut Suastika |
| author_facet | Ni Putu Tesi Maratni Made Ratna Saraswati Ni Nyoman Ayu Dewi I Wayan Putu Sutirta Yasa I Putu Eka Widyadharma Ida Bagus Kusuma Putra Ketut Suastika |
| author_sort | Ni Putu Tesi Maratni |
| collection | DOAJ |
| description | Background. Altered lipid profiles have consistently been linked to cerebrovascular events. Ischemic stroke (IS) was a common comorbid condition established in type 2 diabetes mellitus (T2DM). The apolipoprotein E (ApoE) gene which has a notably critical function in lipoprotein metabolism is believed as one of the potential candidate genes susceptible to IS complications in T2DM. This research aimed to determine the association of apolipoprotein E gene polymorphism with lipid profile and IS risk in T2DM patients. Methods. This case-control study involved a total of 60 diabetic participants divided into two groups with and without IS. ApoE was genotyped using PCR and sequencing analysis. Results. The most predominant genotype observed in 27 participants (45%) was E3/E3. Lower levels of high-density lipoprotein cholesterol (HDL-C) were found in ε2 carriers (p=0.003; 95% CI −23.35–−4.89) and ε4 carriers (p=0.019; 95% CI 1.38–14.55) compared to ε3 homozygotes. Total cholesterol (TC), triglyceride, and low-density lipoprotein cholesterol (LDL-C) levels had no association with ApoE gene polymorphism in this study. ApoE gene polymorphism was not related to IS in T2DM (p=0.06; adjusted OR: 4.71; 95% CI 0.93–23.79). Conclusions. ApoE ε2 and ε4 carriers were associated with lower levels of HDL-C. No association was identified between ApoE gene polymorphism and IS in T2DM patients. |
| format | Article |
| id | doaj-art-91f9a57d62674406bfb06eedf1bd02e6 |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-91f9a57d62674406bfb06eedf1bd02e62025-08-20T03:37:08ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322021-01-01202110.1155/2021/55277365527736Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus PatientsNi Putu Tesi Maratni0Made Ratna Saraswati1Ni Nyoman Ayu Dewi2I Wayan Putu Sutirta Yasa3I Putu Eka Widyadharma4Ida Bagus Kusuma Putra5Ketut Suastika6Doctoral Program of Medical Sciences, Faculty of Medicine, Udayana University, Bali, IndonesiaDepartment of Internal Medicine, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, IndonesiaDepartment of Biochemistry, Faculty of Medicine, Udayana University, Bali, IndonesiaDepartment of Clinical Pathology, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, IndonesiaDepartment of Neurology, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, IndonesiaDepartment of Neurology, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, IndonesiaDepartment of Internal Medicine, Faculty of Medicine, Udayana University, Sanglah General Hospital, Bali, IndonesiaBackground. Altered lipid profiles have consistently been linked to cerebrovascular events. Ischemic stroke (IS) was a common comorbid condition established in type 2 diabetes mellitus (T2DM). The apolipoprotein E (ApoE) gene which has a notably critical function in lipoprotein metabolism is believed as one of the potential candidate genes susceptible to IS complications in T2DM. This research aimed to determine the association of apolipoprotein E gene polymorphism with lipid profile and IS risk in T2DM patients. Methods. This case-control study involved a total of 60 diabetic participants divided into two groups with and without IS. ApoE was genotyped using PCR and sequencing analysis. Results. The most predominant genotype observed in 27 participants (45%) was E3/E3. Lower levels of high-density lipoprotein cholesterol (HDL-C) were found in ε2 carriers (p=0.003; 95% CI −23.35–−4.89) and ε4 carriers (p=0.019; 95% CI 1.38–14.55) compared to ε3 homozygotes. Total cholesterol (TC), triglyceride, and low-density lipoprotein cholesterol (LDL-C) levels had no association with ApoE gene polymorphism in this study. ApoE gene polymorphism was not related to IS in T2DM (p=0.06; adjusted OR: 4.71; 95% CI 0.93–23.79). Conclusions. ApoE ε2 and ε4 carriers were associated with lower levels of HDL-C. No association was identified between ApoE gene polymorphism and IS in T2DM patients.http://dx.doi.org/10.1155/2021/5527736 |
| spellingShingle | Ni Putu Tesi Maratni Made Ratna Saraswati Ni Nyoman Ayu Dewi I Wayan Putu Sutirta Yasa I Putu Eka Widyadharma Ida Bagus Kusuma Putra Ketut Suastika Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients Journal of Nutrition and Metabolism |
| title | Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients |
| title_full | Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients |
| title_fullStr | Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients |
| title_full_unstemmed | Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients |
| title_short | Association of Apolipoprotein E Gene Polymorphism with Lipid Profile and Ischemic Stroke Risk in Type 2 Diabetes Mellitus Patients |
| title_sort | association of apolipoprotein e gene polymorphism with lipid profile and ischemic stroke risk in type 2 diabetes mellitus patients |
| url | http://dx.doi.org/10.1155/2021/5527736 |
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