Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy
Activated anti-oxidation reactions in cells partially diminish the anticancer effect of photodynamic therapy (PDT), significantly hindering efforts to increase the efficacy of PDT. The expression of transcription factor E2 related factor 2 (Nrf2), an important redox-regulated transcription factor, c...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2025-07-01
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| Series: | Fundamental Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667325824001274 |
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| author | Ningyu Li Fan Dong Lisha Sun Yuping Qian Ludan Zhang Guiyan Wang Lintian Yuan Hong Liu Yong Jiang Yuguang Wang |
| author_facet | Ningyu Li Fan Dong Lisha Sun Yuping Qian Ludan Zhang Guiyan Wang Lintian Yuan Hong Liu Yong Jiang Yuguang Wang |
| author_sort | Ningyu Li |
| collection | DOAJ |
| description | Activated anti-oxidation reactions in cells partially diminish the anticancer effect of photodynamic therapy (PDT), significantly hindering efforts to increase the efficacy of PDT. The expression of transcription factor E2 related factor 2 (Nrf2), an important redox-regulated transcription factor, can be downregulated by Nrf2 siRNA, leading to greatly enhanced PDT effects. However, the efficient co-delivery of photosensitizers and siRNAs remains a key problem because these agents are complex to synthesize, exhibit poor biocompatibility and load drugs with a low efficiency. Herein, we designed a carrier–free and extremely simple strategy to co-deliver a photosensitizer and Nrf2 siRNA to cancer cells. In this nanoplatform, an indocyanine green photosensitizer, siRNA and FeⅡ were self-assembled to form a spherical hybrid structure with a uniform size, high loading ratio and adjustable component ratio. The platform can effectively transfer photosensitizers and siRNAs into cells and effectively inhibit tumour growth in vivo. Overall, the self-assembly approach shows great potential for clinical application and provides a simple method to achieve photodynamic therapy and enhanced photothermal therapy. |
| format | Article |
| id | doaj-art-91f43add50564cbea35c70fef8bf07ca |
| institution | DOAJ |
| issn | 2667-3258 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Fundamental Research |
| spelling | doaj-art-91f43add50564cbea35c70fef8bf07ca2025-08-20T03:09:07ZengKeAi Communications Co. Ltd.Fundamental Research2667-32582025-07-01541698170910.1016/j.fmre.2024.03.014Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapyNingyu Li0Fan Dong1Lisha Sun2Yuping Qian3Ludan Zhang4Guiyan Wang5Lintian Yuan6Hong Liu7Yong Jiang8Yuguang Wang9Department of General Dentistry, Hospital of Stomatology, Jilin University, Changchun 130012, ChinaCenter of Digital Dentistry, Department of Prosthodontics, Peking University School and Hospital of Stomatology & National Center of Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology & NHC Research Center of Engineering and Technology for Computerized Dentistry, Beijing 100081, ChinaCentral Laboratory, Peking University School and Hospital of Stomatology, Beijing 100081, ChinaDepartment of General Dentistry II, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, ChinaDepartment of General Dentistry II, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, ChinaDepartment of General Dentistry II, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, ChinaDepartment of General Dentistry II, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, ChinaDepartment of General Dentistry, Hospital of Stomatology, Jilin University, Changchun 130012, China; Corresponding authors.Department of General Dentistry II, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China; Corresponding authors.Department of General Dentistry II, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Beijing 100081, China; Corresponding authors.Activated anti-oxidation reactions in cells partially diminish the anticancer effect of photodynamic therapy (PDT), significantly hindering efforts to increase the efficacy of PDT. The expression of transcription factor E2 related factor 2 (Nrf2), an important redox-regulated transcription factor, can be downregulated by Nrf2 siRNA, leading to greatly enhanced PDT effects. However, the efficient co-delivery of photosensitizers and siRNAs remains a key problem because these agents are complex to synthesize, exhibit poor biocompatibility and load drugs with a low efficiency. Herein, we designed a carrier–free and extremely simple strategy to co-deliver a photosensitizer and Nrf2 siRNA to cancer cells. In this nanoplatform, an indocyanine green photosensitizer, siRNA and FeⅡ were self-assembled to form a spherical hybrid structure with a uniform size, high loading ratio and adjustable component ratio. The platform can effectively transfer photosensitizers and siRNAs into cells and effectively inhibit tumour growth in vivo. Overall, the self-assembly approach shows great potential for clinical application and provides a simple method to achieve photodynamic therapy and enhanced photothermal therapy.http://www.sciencedirect.com/science/article/pii/S2667325824001274Photodynamic therapyPhotothermal therapyNrf2-siRNAIndocyanine greensiRNA delivery |
| spellingShingle | Ningyu Li Fan Dong Lisha Sun Yuping Qian Ludan Zhang Guiyan Wang Lintian Yuan Hong Liu Yong Jiang Yuguang Wang Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy Fundamental Research Photodynamic therapy Photothermal therapy Nrf2-siRNA Indocyanine green siRNA delivery |
| title | Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy |
| title_full | Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy |
| title_fullStr | Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy |
| title_full_unstemmed | Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy |
| title_short | Carrier-free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy |
| title_sort | carrier free delivery of nucleic acid and photosensitizer nanoparticles for enhanced photodynamic and gene antitumour therapy |
| topic | Photodynamic therapy Photothermal therapy Nrf2-siRNA Indocyanine green siRNA delivery |
| url | http://www.sciencedirect.com/science/article/pii/S2667325824001274 |
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