Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort study
IntroductionThe Polycystic ovary syndrome (PCOS), a prevalent endocrine disorder affecting women’s reproductive and metabolic health, faces diagnostic challenges due to heterogeneous clinical presentations and the absence of reliable biomarkers. This study investigates the role of Glucosaminyl (N-ac...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2025.1534733/full |
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| author | Yutong Li Yutong Li Xiufeng Lin Ke Zou Jing Du Qingni Li Linkun Zhong Shan Jiang |
| author_facet | Yutong Li Yutong Li Xiufeng Lin Ke Zou Jing Du Qingni Li Linkun Zhong Shan Jiang |
| author_sort | Yutong Li |
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| description | IntroductionThe Polycystic ovary syndrome (PCOS), a prevalent endocrine disorder affecting women’s reproductive and metabolic health, faces diagnostic challenges due to heterogeneous clinical presentations and the absence of reliable biomarkers. This study investigates the role of Glucosaminyl (N-acetyl) transferase 2 (GCNT2) in modulating sex hormone-binding globulin (SHBG) and its potential as a therapeutic target in PCOS pathophysiology.MethodsA prospective cohort of 103 PCOS patients treated with oral contraceptives (2021–2024) was established. Bidirectional Mendelian randomization (MR) was employed to assess genetic associations and causal relationships between PCOS and SHBG. Molecular docking studies evaluated cryptotanshinone’s binding affinity to key proteins (COL1A1, COL4A2, COL6A2) in the PI3K/Akt pathway. GCNT2’s regulatory effects on collagen synthesis and extracellular matrix pathways. Pharmacokinetic profiling validated therapeutic viability.ResultsBidirectional MR revealed significant genetic associations (P < 0.001) and causal links between PCOS and SHBG, implicating GCNT2 as a key modulator. Cryptotanshinone exhibited strong binding affinity to PI3K/Akt signaling pathway proteins and favorable pharmacokinetic properties. Enrichment analyses highlighted GCNT2’s role in collagen biosynthesis (FDR < 0.05) and extracellular matrix regulation.DiscussionThis study identifies GCNT2 as a critical mediator of PCOS pathophysiology through SHBG modulation and collagen remodeling. Cryptotanshinone emerges as a promising therapeutic candidate, targeting PI3K/Akt signaling pathway with high specificity. These findings advance the understanding of PCOS mechanisms and provide a foundation for biomarker-driven diagnostics and precision therapeutics. Further validation in clinical trials is warranted to translate these insights into practice. |
| format | Article |
| id | doaj-art-91f2d71f850848ecb1d7404bf35a3298 |
| institution | OA Journals |
| issn | 1664-2392 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Endocrinology |
| spelling | doaj-art-91f2d71f850848ecb1d7404bf35a32982025-08-20T01:55:41ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922025-05-011610.3389/fendo.2025.15347331534733Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort studyYutong Li0Yutong Li1Xiufeng Lin2Ke Zou3Jing Du4Qingni Li5Linkun Zhong6Shan Jiang7The First Clinical College, Guangdong Medical University, Zhanjiang, Guangdong, ChinaDepartment of General Surgery, Zhongshan City People’s Hospital, Zhongshan, Guangdong, ChinaReproductive Center, Boai Hospital of Zhongshan, Zhongshan, Guangdong, ChinaThe First Clinical College, Guangdong Medical University, Zhanjiang, Guangdong, ChinaReproductive Center, Boai Hospital of Zhongshan, Zhongshan, Guangdong, ChinaReproductive Center, Boai Hospital of Zhongshan, Zhongshan, Guangdong, ChinaDepartment of General Surgery, Zhongshan City People’s Hospital, Zhongshan, Guangdong, ChinaReproductive Center, Boai Hospital of Zhongshan, Zhongshan, Guangdong, ChinaIntroductionThe Polycystic ovary syndrome (PCOS), a prevalent endocrine disorder affecting women’s reproductive and metabolic health, faces diagnostic challenges due to heterogeneous clinical presentations and the absence of reliable biomarkers. This study investigates the role of Glucosaminyl (N-acetyl) transferase 2 (GCNT2) in modulating sex hormone-binding globulin (SHBG) and its potential as a therapeutic target in PCOS pathophysiology.MethodsA prospective cohort of 103 PCOS patients treated with oral contraceptives (2021–2024) was established. Bidirectional Mendelian randomization (MR) was employed to assess genetic associations and causal relationships between PCOS and SHBG. Molecular docking studies evaluated cryptotanshinone’s binding affinity to key proteins (COL1A1, COL4A2, COL6A2) in the PI3K/Akt pathway. GCNT2’s regulatory effects on collagen synthesis and extracellular matrix pathways. Pharmacokinetic profiling validated therapeutic viability.ResultsBidirectional MR revealed significant genetic associations (P < 0.001) and causal links between PCOS and SHBG, implicating GCNT2 as a key modulator. Cryptotanshinone exhibited strong binding affinity to PI3K/Akt signaling pathway proteins and favorable pharmacokinetic properties. Enrichment analyses highlighted GCNT2’s role in collagen biosynthesis (FDR < 0.05) and extracellular matrix regulation.DiscussionThis study identifies GCNT2 as a critical mediator of PCOS pathophysiology through SHBG modulation and collagen remodeling. Cryptotanshinone emerges as a promising therapeutic candidate, targeting PI3K/Akt signaling pathway with high specificity. These findings advance the understanding of PCOS mechanisms and provide a foundation for biomarker-driven diagnostics and precision therapeutics. Further validation in clinical trials is warranted to translate these insights into practice.https://www.frontiersin.org/articles/10.3389/fendo.2025.1534733/fullcryptotanshinoneglucosaminyl (N-acetyl) transferase 2SHBGpolycystic ovary syndromeMendelian randomization (MR) |
| spellingShingle | Yutong Li Yutong Li Xiufeng Lin Ke Zou Jing Du Qingni Li Linkun Zhong Shan Jiang Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort study Frontiers in Endocrinology cryptotanshinone glucosaminyl (N-acetyl) transferase 2 SHBG polycystic ovary syndrome Mendelian randomization (MR) |
| title | Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort study |
| title_full | Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort study |
| title_fullStr | Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort study |
| title_full_unstemmed | Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort study |
| title_short | Blood biochemical landscape and new insights into clinical decision-making for polycystic ovary syndrome in Chinese women: a prospective cohort study |
| title_sort | blood biochemical landscape and new insights into clinical decision making for polycystic ovary syndrome in chinese women a prospective cohort study |
| topic | cryptotanshinone glucosaminyl (N-acetyl) transferase 2 SHBG polycystic ovary syndrome Mendelian randomization (MR) |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2025.1534733/full |
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