Distance in cancer gene expression from stem cells predicts patient survival.

The degree of histologic cellular differentiation of a cancer has been associated with prognosis but is subjectively assessed. We hypothesized that information about tumor differentiation of individual cancers could be derived objectively from cancer gene expression data, and would allow creation of...

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Main Authors: Markus Riester, Hua-Jun Wu, Ahmet Zehir, Mithat Gönen, Andre L Moreira, Robert J Downey, Franziska Michor
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2017-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0173589
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author Markus Riester
Hua-Jun Wu
Ahmet Zehir
Mithat Gönen
Andre L Moreira
Robert J Downey
Franziska Michor
author_facet Markus Riester
Hua-Jun Wu
Ahmet Zehir
Mithat Gönen
Andre L Moreira
Robert J Downey
Franziska Michor
author_sort Markus Riester
collection DOAJ
description The degree of histologic cellular differentiation of a cancer has been associated with prognosis but is subjectively assessed. We hypothesized that information about tumor differentiation of individual cancers could be derived objectively from cancer gene expression data, and would allow creation of a cancer phylogenetic framework that would correlate with clinical, histologic and molecular characteristics of the cancers, as well as predict prognosis. Here we utilized mRNA expression data from 4,413 patient samples with 7 diverse cancer histologies to explore the utility of ordering samples by their distance in gene expression from that of stem cells. A differentiation baseline was obtained by including expression data of human embryonic stem cells (hESC) and human mesenchymal stem cells (hMSC) for solid tumors, and of hESC and CD34+ cells for liquid tumors. We found that the correlation distance (the degree of similarity) between the gene expression profile of a tumor sample and that of stem cells orients cancers in a clinically coherent fashion. For all histologies analyzed (including carcinomas, sarcomas, and hematologic malignancies), patients with cancers with gene expression patterns most similar to that of stem cells had poorer overall survival. We also found that the genes in all undifferentiated cancers of diverse histologies that were most differentially expressed were associated with up-regulation of specific oncogenes and down-regulation of specific tumor suppressor genes. Thus, a stem cell-oriented phylogeny of cancers allows for the derivation of a novel cancer gene expression signature found in all undifferentiated forms of diverse cancer histologies, that is competitive in predicting overall survival in cancer patients compared to previously published prediction models, and is coherent in that gene expression was associated with up-regulation of specific oncogenes and down-regulation of specific tumor suppressor genes associated with regulation of the multicellular state.
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spelling doaj-art-91ed221401b54d5fb9766fa47c71ff872025-08-20T03:32:15ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01123e017358910.1371/journal.pone.0173589Distance in cancer gene expression from stem cells predicts patient survival.Markus RiesterHua-Jun WuAhmet ZehirMithat GönenAndre L MoreiraRobert J DowneyFranziska MichorThe degree of histologic cellular differentiation of a cancer has been associated with prognosis but is subjectively assessed. We hypothesized that information about tumor differentiation of individual cancers could be derived objectively from cancer gene expression data, and would allow creation of a cancer phylogenetic framework that would correlate with clinical, histologic and molecular characteristics of the cancers, as well as predict prognosis. Here we utilized mRNA expression data from 4,413 patient samples with 7 diverse cancer histologies to explore the utility of ordering samples by their distance in gene expression from that of stem cells. A differentiation baseline was obtained by including expression data of human embryonic stem cells (hESC) and human mesenchymal stem cells (hMSC) for solid tumors, and of hESC and CD34+ cells for liquid tumors. We found that the correlation distance (the degree of similarity) between the gene expression profile of a tumor sample and that of stem cells orients cancers in a clinically coherent fashion. For all histologies analyzed (including carcinomas, sarcomas, and hematologic malignancies), patients with cancers with gene expression patterns most similar to that of stem cells had poorer overall survival. We also found that the genes in all undifferentiated cancers of diverse histologies that were most differentially expressed were associated with up-regulation of specific oncogenes and down-regulation of specific tumor suppressor genes. Thus, a stem cell-oriented phylogeny of cancers allows for the derivation of a novel cancer gene expression signature found in all undifferentiated forms of diverse cancer histologies, that is competitive in predicting overall survival in cancer patients compared to previously published prediction models, and is coherent in that gene expression was associated with up-regulation of specific oncogenes and down-regulation of specific tumor suppressor genes associated with regulation of the multicellular state.https://doi.org/10.1371/journal.pone.0173589
spellingShingle Markus Riester
Hua-Jun Wu
Ahmet Zehir
Mithat Gönen
Andre L Moreira
Robert J Downey
Franziska Michor
Distance in cancer gene expression from stem cells predicts patient survival.
PLoS ONE
title Distance in cancer gene expression from stem cells predicts patient survival.
title_full Distance in cancer gene expression from stem cells predicts patient survival.
title_fullStr Distance in cancer gene expression from stem cells predicts patient survival.
title_full_unstemmed Distance in cancer gene expression from stem cells predicts patient survival.
title_short Distance in cancer gene expression from stem cells predicts patient survival.
title_sort distance in cancer gene expression from stem cells predicts patient survival
url https://doi.org/10.1371/journal.pone.0173589
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AT mithatgonen distanceincancergeneexpressionfromstemcellspredictspatientsurvival
AT andrelmoreira distanceincancergeneexpressionfromstemcellspredictspatientsurvival
AT robertjdowney distanceincancergeneexpressionfromstemcellspredictspatientsurvival
AT franziskamichor distanceincancergeneexpressionfromstemcellspredictspatientsurvival