Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug Discovery
Periodontal disease is a pervasive and serious health issue, affecting millions globally and leading to severe oral and systemic health complications. This underscores the urgent need to thoroughly understand the complex host-microbe interactions involved. Developing models that allow crosstalk amon...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-03-01
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| Series: | Journal of Tissue Engineering |
| Online Access: | https://doi.org/10.1177/20417314251314356 |
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| author | Qin Hu Aneesha Acharya Ho Cheung Shum Wai Keung Leung George Pelekos |
| author_facet | Qin Hu Aneesha Acharya Ho Cheung Shum Wai Keung Leung George Pelekos |
| author_sort | Qin Hu |
| collection | DOAJ |
| description | Periodontal disease is a pervasive and serious health issue, affecting millions globally and leading to severe oral and systemic health complications. This underscores the urgent need to thoroughly understand the complex host-microbe interactions involved. Developing models that allow crosstalk among various bacteria, periodontal component cells, and circulating immune cells is crucial for investigating periodontal disease and discovering new treatments. This study aimed to develop a biomimetic gum tissue model. Within four days, a bio-fabricated tissue with well-established barrier and immune functions was created. In this model, the key periodontal pathogen, Porphyromonas gingivalis , was observed to suppress the recruitment and migration of immune cells and dysregulate CD14 expression in THP-1 cells, leading to significant inflammation and tissue damage. Conversely, the probiotic Akkermansia muciniphila enhanced the host’s defensive immune response, highlighting its potential as a therapeutic agent in periodontal disease. |
| format | Article |
| id | doaj-art-91e80477efb549d69b00201c45a29a73 |
| institution | DOAJ |
| issn | 2041-7314 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Tissue Engineering |
| spelling | doaj-art-91e80477efb549d69b00201c45a29a732025-08-20T02:52:55ZengSAGE PublishingJournal of Tissue Engineering2041-73142025-03-011610.1177/20417314251314356Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug DiscoveryQin Hu0Aneesha Acharya1Ho Cheung Shum2Wai Keung Leung3George Pelekos4The University of Hong Kong, Sai Ying Pun, Hong Kong SAR, ChinaDr D Y Patil Dental College and Hospital, Pune, IndiaAdvanced Biomedical Instrumentation Centre, Hong Kong Science Park, Shatin, Hong Kong SAR, ChinaThe University of Hong Kong, Sai Ying Pun, Hong Kong SAR, ChinaThe University of Hong Kong, Sai Ying Pun, Hong Kong SAR, ChinaPeriodontal disease is a pervasive and serious health issue, affecting millions globally and leading to severe oral and systemic health complications. This underscores the urgent need to thoroughly understand the complex host-microbe interactions involved. Developing models that allow crosstalk among various bacteria, periodontal component cells, and circulating immune cells is crucial for investigating periodontal disease and discovering new treatments. This study aimed to develop a biomimetic gum tissue model. Within four days, a bio-fabricated tissue with well-established barrier and immune functions was created. In this model, the key periodontal pathogen, Porphyromonas gingivalis , was observed to suppress the recruitment and migration of immune cells and dysregulate CD14 expression in THP-1 cells, leading to significant inflammation and tissue damage. Conversely, the probiotic Akkermansia muciniphila enhanced the host’s defensive immune response, highlighting its potential as a therapeutic agent in periodontal disease.https://doi.org/10.1177/20417314251314356 |
| spellingShingle | Qin Hu Aneesha Acharya Ho Cheung Shum Wai Keung Leung George Pelekos Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug Discovery Journal of Tissue Engineering |
| title | Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug Discovery |
| title_full | Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug Discovery |
| title_fullStr | Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug Discovery |
| title_full_unstemmed | Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug Discovery |
| title_short | Gum-on-a-Chip Exploring Host-Microbe Interactions: Periodontal Disease Modeling and Drug Discovery |
| title_sort | gum on a chip exploring host microbe interactions periodontal disease modeling and drug discovery |
| url | https://doi.org/10.1177/20417314251314356 |
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