Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by Cyclophosphamide
Background. Lepidium meyenii Walp. (Maca) has emerged as a functional plant food and traditional herb owing to its biological activities; Maca polysaccharides as an important active component of Maca have good immunomodulatory effect; however, studies on the immunomodulatory effect of Maca polysacch...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/1210890 |
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| author | Wen-ting Fei Na Yue Ai-min Li Shu-hui Yu Dan-ping Zhao Ying-li Zhu Chun Wang Jian-jun Zhang Lin-yuan Wang |
| author_facet | Wen-ting Fei Na Yue Ai-min Li Shu-hui Yu Dan-ping Zhao Ying-li Zhu Chun Wang Jian-jun Zhang Lin-yuan Wang |
| author_sort | Wen-ting Fei |
| collection | DOAJ |
| description | Background. Lepidium meyenii Walp. (Maca) has emerged as a functional plant food and traditional herb owing to its biological activities; Maca polysaccharides as an important active component of Maca have good immunomodulatory effect; however, studies on the immunomodulatory effect of Maca polysaccharides are mainly focused on macrophages; little attention has been devoted to the mechanisms and other immune cells. This study is aimed at investigating the immunomodulatory effects and mechanisms of Maca polysaccharides. Methods. Sixty mice were divided into five groups, and the mice were injected with cyclophosphamide to establish an immunosuppression model except for those in the common group. The body weights were measured, as well as immune-related indices, such as organ indices, haematological parameters, lymphocyte cycle, and proliferation, cytokine, and protein expression levels. Results. The weight loss and immune organ index decline caused by cyclophosphamide could be reversed by MP. Furthermore, MP increased WBC and HGB counts and reduced the ratio of G0/G1 phase obviously, increased the proportion of S phase and G2/M phase in peripheral blood lymphocytes, increased the counts of CD4+ T cells and the ratio of CD4+/CD8+, and reduced the inhibition rate of splenic lymphocytes. MP affected the production of cytokines by increasing IFN-γ, TNF-α, and IL-2 levels and by decreasing IL-4 levels. MP increased the mRNA expression of T-bet and the protein expression of Bcl-2 in the spleen and decreased the protein expression of caspase-3 and Bax. Conclusions. Maca polysaccharides might be the basic material for Maca’s immunomodulatory effect. The mechanism was perhaps related to inhibiting lymphocyte apoptosis and promoting the balance of Th1/Th2 cell subsets. |
| format | Article |
| id | doaj-art-91e78fb1643d4b37b2bbffb4728f6cf0 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-91e78fb1643d4b37b2bbffb4728f6cf02025-08-20T03:37:08ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/1210890Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by CyclophosphamideWen-ting Fei0Na Yue1Ai-min Li2Shu-hui Yu3Dan-ping Zhao4Ying-li Zhu5Chun Wang6Jian-jun Zhang7Lin-yuan Wang8School of Chinese Materia MedicaSchool of Traditional Chinese MedicineNew Era Health Industry (Group) Co.School of Traditional Chinese MedicineSchool of Chinese Materia MedicaSchool of Chinese Materia MedicaSchool of Traditional Chinese MedicineSchool of Traditional Chinese MedicineSchool of Chinese Materia MedicaBackground. Lepidium meyenii Walp. (Maca) has emerged as a functional plant food and traditional herb owing to its biological activities; Maca polysaccharides as an important active component of Maca have good immunomodulatory effect; however, studies on the immunomodulatory effect of Maca polysaccharides are mainly focused on macrophages; little attention has been devoted to the mechanisms and other immune cells. This study is aimed at investigating the immunomodulatory effects and mechanisms of Maca polysaccharides. Methods. Sixty mice were divided into five groups, and the mice were injected with cyclophosphamide to establish an immunosuppression model except for those in the common group. The body weights were measured, as well as immune-related indices, such as organ indices, haematological parameters, lymphocyte cycle, and proliferation, cytokine, and protein expression levels. Results. The weight loss and immune organ index decline caused by cyclophosphamide could be reversed by MP. Furthermore, MP increased WBC and HGB counts and reduced the ratio of G0/G1 phase obviously, increased the proportion of S phase and G2/M phase in peripheral blood lymphocytes, increased the counts of CD4+ T cells and the ratio of CD4+/CD8+, and reduced the inhibition rate of splenic lymphocytes. MP affected the production of cytokines by increasing IFN-γ, TNF-α, and IL-2 levels and by decreasing IL-4 levels. MP increased the mRNA expression of T-bet and the protein expression of Bcl-2 in the spleen and decreased the protein expression of caspase-3 and Bax. Conclusions. Maca polysaccharides might be the basic material for Maca’s immunomodulatory effect. The mechanism was perhaps related to inhibiting lymphocyte apoptosis and promoting the balance of Th1/Th2 cell subsets.http://dx.doi.org/10.1155/2022/1210890 |
| spellingShingle | Wen-ting Fei Na Yue Ai-min Li Shu-hui Yu Dan-ping Zhao Ying-li Zhu Chun Wang Jian-jun Zhang Lin-yuan Wang Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by Cyclophosphamide Journal of Immunology Research |
| title | Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by Cyclophosphamide |
| title_full | Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by Cyclophosphamide |
| title_fullStr | Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by Cyclophosphamide |
| title_full_unstemmed | Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by Cyclophosphamide |
| title_short | Immunomodulatory Effects of Lepidium meyenii Walp. Polysaccharides on an Immunosuppression Model Induced by Cyclophosphamide |
| title_sort | immunomodulatory effects of lepidium meyenii walp polysaccharides on an immunosuppression model induced by cyclophosphamide |
| url | http://dx.doi.org/10.1155/2022/1210890 |
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