A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancer
Abstract Esophageal cancer is one of the most common cancers and a leading cause of cancer‐related death worldwide. However, the mechanism of esophageal cancer pathogenesis remains poorly understood. Long noncoding RNAs (lncRNAs) dysregulation have been reported to involve in various human cancers,...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-08-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.1536 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850122613702000640 |
|---|---|
| author | Wenjia Liu Yiyang Zhang Min Chen Liangliang Shi Lei Xu Xiaoping Zou |
| author_facet | Wenjia Liu Yiyang Zhang Min Chen Liangliang Shi Lei Xu Xiaoping Zou |
| author_sort | Wenjia Liu |
| collection | DOAJ |
| description | Abstract Esophageal cancer is one of the most common cancers and a leading cause of cancer‐related death worldwide. However, the mechanism of esophageal cancer pathogenesis remains poorly understood. Long noncoding RNAs (lncRNAs) dysregulation have been reported to involve in various human cancers, which highlights the potential of lncRNAs used as novel biomarkers for cancer diagnosis. Although more efforts have been made to identify novel lncRNAs signature in esophageal cancer, the expression pattern, prognostic value, and biological function of most lncRNAs in esophageal cancer still need to be systematically investigated. In this study, we comprehensively analyzed the expression profile of lncRNAs in more than 200 esophageal cancer patients tissue samples from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). We identified thousands of lncRNAs are differentially expressed in esophageal cancer tissues, and many of those lncRNAs expression are associated with patients overall survival or recurrence‐free survival time. Moreover, copy number variation analyses revealed that genomic loci copy number amplification or deletion might contribute to these lncRNAs dysregulation. Among these lncRNAs, DUXAP8 and LINC00460 were significantly upregulated, and GO enrichment analyses indicated that the two lncRNAs associated protein‐coding genes involve with many known biological processes, such as cell cycle and cell‐cell adherens junction. Further experimental validation revealed that knockdown of DUXAP8 could impair esophageal cancer cells proliferation and invasion in vitro. Taken together, our findings identified more aberrantly expressed lncRNAs in esophageal cancer that may provide a useful resource for identifying novel esophageal cancer associated lncRNAs. |
| format | Article |
| id | doaj-art-91e779f9df784e288b2909bbe28b9a8d |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2018-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-91e779f9df784e288b2909bbe28b9a8d2025-08-20T02:34:47ZengWileyCancer Medicine2045-76342018-08-01784181418910.1002/cam4.1536A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancerWenjia Liu0Yiyang Zhang1Min Chen2Liangliang Shi3Lei Xu4Xiaoping Zou5Department of Gastroenterology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University Nanjing ChinaDepartment of Gastroenterology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University Nanjing ChinaDepartment of Gastroenterology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University Nanjing ChinaDepartment of Gastroenterology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University Nanjing ChinaDepartment of Gastroenterology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University Nanjing ChinaDepartment of Gastroenterology Nanjing Drum Tower Hospital Clinical College of Nanjing Medical University Nanjing ChinaAbstract Esophageal cancer is one of the most common cancers and a leading cause of cancer‐related death worldwide. However, the mechanism of esophageal cancer pathogenesis remains poorly understood. Long noncoding RNAs (lncRNAs) dysregulation have been reported to involve in various human cancers, which highlights the potential of lncRNAs used as novel biomarkers for cancer diagnosis. Although more efforts have been made to identify novel lncRNAs signature in esophageal cancer, the expression pattern, prognostic value, and biological function of most lncRNAs in esophageal cancer still need to be systematically investigated. In this study, we comprehensively analyzed the expression profile of lncRNAs in more than 200 esophageal cancer patients tissue samples from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). We identified thousands of lncRNAs are differentially expressed in esophageal cancer tissues, and many of those lncRNAs expression are associated with patients overall survival or recurrence‐free survival time. Moreover, copy number variation analyses revealed that genomic loci copy number amplification or deletion might contribute to these lncRNAs dysregulation. Among these lncRNAs, DUXAP8 and LINC00460 were significantly upregulated, and GO enrichment analyses indicated that the two lncRNAs associated protein‐coding genes involve with many known biological processes, such as cell cycle and cell‐cell adherens junction. Further experimental validation revealed that knockdown of DUXAP8 could impair esophageal cancer cells proliferation and invasion in vitro. Taken together, our findings identified more aberrantly expressed lncRNAs in esophageal cancer that may provide a useful resource for identifying novel esophageal cancer associated lncRNAs.https://doi.org/10.1002/cam4.1536cell proliferationDUXAP8esophageal cancerlncRNAsprofiling |
| spellingShingle | Wenjia Liu Yiyang Zhang Min Chen Liangliang Shi Lei Xu Xiaoping Zou A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancer Cancer Medicine cell proliferation DUXAP8 esophageal cancer lncRNAs profiling |
| title | A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancer |
| title_full | A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancer |
| title_fullStr | A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancer |
| title_full_unstemmed | A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancer |
| title_short | A genome‐wide analysis of long noncoding RNA profile identifies differentially expressed lncRNAs associated with Esophageal cancer |
| title_sort | genome wide analysis of long noncoding rna profile identifies differentially expressed lncrnas associated with esophageal cancer |
| topic | cell proliferation DUXAP8 esophageal cancer lncRNAs profiling |
| url | https://doi.org/10.1002/cam4.1536 |
| work_keys_str_mv | AT wenjialiu agenomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT yiyangzhang agenomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT minchen agenomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT liangliangshi agenomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT leixu agenomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT xiaopingzou agenomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT wenjialiu genomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT yiyangzhang genomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT minchen genomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT liangliangshi genomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT leixu genomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer AT xiaopingzou genomewideanalysisoflongnoncodingrnaprofileidentifiesdifferentiallyexpressedlncrnasassociatedwithesophagealcancer |