The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying
In the present eco-conscious era, consumers opt for food choices reflecting ethical and environmental concerns, which increases the demand for organic products. Biocontrol is a viable plant protection method in organic farming. Freeze-drying is a long-term preservation technique for microbial agents...
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Polish Academy of Sciences
2024-11-01
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| Series: | Journal of Plant Protection Research |
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| Online Access: | https://journals.pan.pl/Content/133854/PDF/OA_4_JPPR_64_4_2006_Krzyminska.pdf |
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| author | Joanna Krzymińska Jolanta Kowalska |
| author_facet | Joanna Krzymińska Jolanta Kowalska |
| author_sort | Joanna Krzymińska |
| collection | DOAJ |
| description | In the present eco-conscious era, consumers opt for food choices reflecting ethical and environmental concerns, which increases the demand for organic products. Biocontrol is a viable plant protection method in organic farming. Freeze-drying is a long-term preservation technique for microbial agents, ensuring their genetic stability and viability. To reduce freeze-drying-induced damage to their cells, cryoprotective agents like trehalose and monosodium glutamate are used. This study evaluated the impact of the addition of these substances during the freeze-drying process on chosen yeast isolates’ viability, their ability to survive on tomato leaves and maintain antagonistic properties against Botrytis cinerea Pers. Yeast isolates 114/73 (Wickerhamomyces anomalus E.C. Hansen) and 117/10 (Naganishia albidosimilis Vishniac & Kurtzman) were tested on tomato plants under greenhouse conditions before and after the freeze-drying process for both the ability to colonize leaves and as a preventive and interventional treatment against B. cinerea. Yeast viability post freeze-drying was evaluated in vitro. Both trehalose and monosodium glutamate increased yeast viability during the freeze-drying process. Viability was not very high (from 30.33 to 36.17% for 117/10 and from 10.67 to 16.5% for 114/73). Yeast dehydrated after freeze-drying, protected with trehalose and monosodium glutamate, displayed the same colony count on tomato leaves as before freeze-drying. The efficacy of protective treatments depended on the yeast isolate, the protective substance used during freeze-drying, treatment timing (prevention vs. intervention), and interactions of those factors. Cryopreserved isolate 117/10 performed better than 114/73 with the addition of either trehalose or monosodium glutamate, reducing the disease severity index from 88.3% (control) to 18.75−55.33%. Preventive treatments were more efficient than intervention. The leaf colonization ability and biocontrol efficacy of yeast isolates against B. cinerea post-freeze-drying offer promising solutions in sustainable agriculture. However, further research, to analyze the interactions between various factors and to optimize strategies may be needed. |
| format | Article |
| id | doaj-art-91dea645940542808b492dcc3a021dcd |
| institution | DOAJ |
| issn | 1427-4345 1899-007X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Polish Academy of Sciences |
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| series | Journal of Plant Protection Research |
| spelling | doaj-art-91dea645940542808b492dcc3a021dcd2025-08-20T02:47:46ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007X2024-11-01vol. 64No 4https://doi.org/10.24425/jppr.2024.151822The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-dryingJoanna Krzymińska0https://orcid.org/0000-0002-6257-9850Jolanta Kowalska1https://orcid.org/0000-0002-0588-7355Department of Organic Agriculture and Environmental Protection, Institute of Plant Protection – National Research Institute, Poznań, PolandDepartment of Organic Agriculture and Environmental Protection, Institute of Plant Protection – National Research Institute, Poznań, PolandIn the present eco-conscious era, consumers opt for food choices reflecting ethical and environmental concerns, which increases the demand for organic products. Biocontrol is a viable plant protection method in organic farming. Freeze-drying is a long-term preservation technique for microbial agents, ensuring their genetic stability and viability. To reduce freeze-drying-induced damage to their cells, cryoprotective agents like trehalose and monosodium glutamate are used. This study evaluated the impact of the addition of these substances during the freeze-drying process on chosen yeast isolates’ viability, their ability to survive on tomato leaves and maintain antagonistic properties against Botrytis cinerea Pers. Yeast isolates 114/73 (Wickerhamomyces anomalus E.C. Hansen) and 117/10 (Naganishia albidosimilis Vishniac & Kurtzman) were tested on tomato plants under greenhouse conditions before and after the freeze-drying process for both the ability to colonize leaves and as a preventive and interventional treatment against B. cinerea. Yeast viability post freeze-drying was evaluated in vitro. Both trehalose and monosodium glutamate increased yeast viability during the freeze-drying process. Viability was not very high (from 30.33 to 36.17% for 117/10 and from 10.67 to 16.5% for 114/73). Yeast dehydrated after freeze-drying, protected with trehalose and monosodium glutamate, displayed the same colony count on tomato leaves as before freeze-drying. The efficacy of protective treatments depended on the yeast isolate, the protective substance used during freeze-drying, treatment timing (prevention vs. intervention), and interactions of those factors. Cryopreserved isolate 117/10 performed better than 114/73 with the addition of either trehalose or monosodium glutamate, reducing the disease severity index from 88.3% (control) to 18.75−55.33%. Preventive treatments were more efficient than intervention. The leaf colonization ability and biocontrol efficacy of yeast isolates against B. cinerea post-freeze-drying offer promising solutions in sustainable agriculture. However, further research, to analyze the interactions between various factors and to optimize strategies may be needed.https://journals.pan.pl/Content/133854/PDF/OA_4_JPPR_64_4_2006_Krzyminska.pdfbiocontrolbotrytis cinereafreeze-drytomatoyeast |
| spellingShingle | Joanna Krzymińska Jolanta Kowalska The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying Journal of Plant Protection Research biocontrol botrytis cinerea freeze-dry tomato yeast |
| title | The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying |
| title_full | The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying |
| title_fullStr | The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying |
| title_full_unstemmed | The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying |
| title_short | The protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze-drying |
| title_sort | protective effect of trehalose and monosodium glutamate on yeast viability and antagonistic properties during freeze drying |
| topic | biocontrol botrytis cinerea freeze-dry tomato yeast |
| url | https://journals.pan.pl/Content/133854/PDF/OA_4_JPPR_64_4_2006_Krzyminska.pdf |
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