Occurrence of Bordetella Infection in Pigs in Northern India
Bordetella bronchiseptica infection causing atrophic rhinitis in pigs is reported from almost all countries. In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2014/238575 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567389153132544 |
|---|---|
| author | Sandeep Kumar Bhoj R. Singh Monika Bhardwaj Vidya Singh |
| author_facet | Sandeep Kumar Bhoj R. Singh Monika Bhardwaj Vidya Singh |
| author_sort | Sandeep Kumar |
| collection | DOAJ |
| description | Bordetella bronchiseptica infection causing atrophic rhinitis in pigs is reported from almost all countries. In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). Bordetella bronchiseptica could be isolated from six (1.92%) nasal swabs. Although isolates varied significantly in their antimicrobial sensitivity, they had similar plasmid profile. The genus specific and species specific amplicons were detected from 8.2% and 4.4% nasal swabs using mPCR with alc gene (genus specific) and fla gene and fim2 gene (species specific) primers, respectively. Observations revealed that there may be other bordetellae infecting pigs because about 50% of the samples positive using mPCR for genus specific amplicons failed to confirm presence of B. bronchiseptica. Of the pig sera tested with MAT and ELISA for Bordetella antibodies, 67.6% and 86.3% samples, respectively, were positive. For antigen detection mPCR was more sensitive than conventional bacterioscopy while for detection of antibodies neither of the two tests (MAT and ELISA) had specificity in relation to antigen detection. Study indicated high prevalence of infection in swine herds in Northern India. |
| format | Article |
| id | doaj-art-91de412bc7df4eb7b8c3e49153d8f358 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-91de412bc7df4eb7b8c3e49153d8f3582025-02-03T01:01:41ZengWileyInternational Journal of Microbiology1687-918X1687-91982014-01-01201410.1155/2014/238575238575Occurrence of Bordetella Infection in Pigs in Northern IndiaSandeep Kumar0Bhoj R. Singh1Monika Bhardwaj2Vidya Singh3Section of Epidemiology, Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly 243122, IndiaSection of Epidemiology, Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly 243122, IndiaSection of Epidemiology, Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly 243122, IndiaSection of Epidemiology, Centre for Animal Disease Research and Diagnosis (CADRAD), Indian Veterinary Research Institute (IVRI), Izatnagar, Bareilly 243122, IndiaBordetella bronchiseptica infection causing atrophic rhinitis in pigs is reported from almost all countries. In the present study, occurrence of Bordetella infection in apparently healthy pigs was determined in 392 pigs sampled to collect 358 serum samples and 316 nasal swabs from Northern India by conventional bacterioscopy, detection of antigen with multiplex polymerase chain reaction (mPCR), and detection of antibodies with microagglutination test (MAT) and enzyme linked immune-sorbent assay (ELISA). Bordetella bronchiseptica could be isolated from six (1.92%) nasal swabs. Although isolates varied significantly in their antimicrobial sensitivity, they had similar plasmid profile. The genus specific and species specific amplicons were detected from 8.2% and 4.4% nasal swabs using mPCR with alc gene (genus specific) and fla gene and fim2 gene (species specific) primers, respectively. Observations revealed that there may be other bordetellae infecting pigs because about 50% of the samples positive using mPCR for genus specific amplicons failed to confirm presence of B. bronchiseptica. Of the pig sera tested with MAT and ELISA for Bordetella antibodies, 67.6% and 86.3% samples, respectively, were positive. For antigen detection mPCR was more sensitive than conventional bacterioscopy while for detection of antibodies neither of the two tests (MAT and ELISA) had specificity in relation to antigen detection. Study indicated high prevalence of infection in swine herds in Northern India.http://dx.doi.org/10.1155/2014/238575 |
| spellingShingle | Sandeep Kumar Bhoj R. Singh Monika Bhardwaj Vidya Singh Occurrence of Bordetella Infection in Pigs in Northern India International Journal of Microbiology |
| title | Occurrence of Bordetella Infection in Pigs in Northern India |
| title_full | Occurrence of Bordetella Infection in Pigs in Northern India |
| title_fullStr | Occurrence of Bordetella Infection in Pigs in Northern India |
| title_full_unstemmed | Occurrence of Bordetella Infection in Pigs in Northern India |
| title_short | Occurrence of Bordetella Infection in Pigs in Northern India |
| title_sort | occurrence of bordetella infection in pigs in northern india |
| url | http://dx.doi.org/10.1155/2014/238575 |
| work_keys_str_mv | AT sandeepkumar occurrenceofbordetellainfectioninpigsinnorthernindia AT bhojrsingh occurrenceofbordetellainfectioninpigsinnorthernindia AT monikabhardwaj occurrenceofbordetellainfectioninpigsinnorthernindia AT vidyasingh occurrenceofbordetellainfectioninpigsinnorthernindia |