PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression
Background. This work focused on investigating the role of programmed death ligand 2 (PD-L2) in the progression of breast cancer by utilizing breast cancer specimens and cells. Materials and Methods. The serum levels of soluble PD-L2 (sPD-L2) in breast cancer patients and healthy individuals were an...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2024/3145695 |
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| author | Yuling Sun Jie Yang Yachun Chen Yundi Guo Jian Xiong Xuqin Guo Yawen Zhang Li Gu Min Tong Weipeng Wang Jing Sun |
| author_facet | Yuling Sun Jie Yang Yachun Chen Yundi Guo Jian Xiong Xuqin Guo Yawen Zhang Li Gu Min Tong Weipeng Wang Jing Sun |
| author_sort | Yuling Sun |
| collection | DOAJ |
| description | Background. This work focused on investigating the role of programmed death ligand 2 (PD-L2) in the progression of breast cancer by utilizing breast cancer specimens and cells. Materials and Methods. The serum levels of soluble PD-L2 (sPD-L2) in breast cancer patients and healthy individuals were analyzed by means of the enzyme-linked immunosorbent assay, and the PD-L2 levels within 416 resected breast cancer specimens were assessed through immunohistochemistry. Concurrently, in vitro cell experiments and in vivo animal experiments were carried out to analyze the relationship between PD-L2 and the invasion and migration of breast cancer. Results. The concentration of sPD-L2 in breast cancer patients significantly increased compared to that in the control groups. Additionally, breast cancer patients with high concentrations of sPD-L2 had higher Ki67 values (≥30%) and tumor grades. PD-L2 was expressed in 79.09% of the cancer samples, which exhibited a positive correlation with the progesterone receptor (PR) and the human epidermal growth factor receptor 2 (HER2). Furthermore, we discovered that knockdown of PD-L2 inhibited the migratory and invasive abilities of both MCF-7 and MDA-MB231 cells. Conclusion. Our findings demonstrated that knockdown of PD-L2 suppressed tumor growth, providing novel insights into important biological functions. |
| format | Article |
| id | doaj-art-91d6d2f69f7645258ad424fa17d1dc28 |
| institution | OA Journals |
| issn | 2314-7156 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-91d6d2f69f7645258ad424fa17d1dc282025-08-20T02:10:02ZengWileyJournal of Immunology Research2314-71562024-01-01202410.1155/2024/3145695PD-L2 Expression in Breast Cancer Promotes Tumor Development and ProgressionYuling Sun0Jie Yang1Yachun Chen2Yundi Guo3Jian Xiong4Xuqin Guo5Yawen Zhang6Li Gu7Min Tong8Weipeng Wang9Jing Sun10Jiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyCenter for Drug Metabolism and PharmacokineticsCenter for Drug Metabolism and PharmacokineticsJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyCenter for Drug Metabolism and PharmacokineticsJiangsu Province Engineering Research Center of Molecular Target Therapy and Companion Diagnostics in OncologyBackground. This work focused on investigating the role of programmed death ligand 2 (PD-L2) in the progression of breast cancer by utilizing breast cancer specimens and cells. Materials and Methods. The serum levels of soluble PD-L2 (sPD-L2) in breast cancer patients and healthy individuals were analyzed by means of the enzyme-linked immunosorbent assay, and the PD-L2 levels within 416 resected breast cancer specimens were assessed through immunohistochemistry. Concurrently, in vitro cell experiments and in vivo animal experiments were carried out to analyze the relationship between PD-L2 and the invasion and migration of breast cancer. Results. The concentration of sPD-L2 in breast cancer patients significantly increased compared to that in the control groups. Additionally, breast cancer patients with high concentrations of sPD-L2 had higher Ki67 values (≥30%) and tumor grades. PD-L2 was expressed in 79.09% of the cancer samples, which exhibited a positive correlation with the progesterone receptor (PR) and the human epidermal growth factor receptor 2 (HER2). Furthermore, we discovered that knockdown of PD-L2 inhibited the migratory and invasive abilities of both MCF-7 and MDA-MB231 cells. Conclusion. Our findings demonstrated that knockdown of PD-L2 suppressed tumor growth, providing novel insights into important biological functions.http://dx.doi.org/10.1155/2024/3145695 |
| spellingShingle | Yuling Sun Jie Yang Yachun Chen Yundi Guo Jian Xiong Xuqin Guo Yawen Zhang Li Gu Min Tong Weipeng Wang Jing Sun PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression Journal of Immunology Research |
| title | PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression |
| title_full | PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression |
| title_fullStr | PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression |
| title_full_unstemmed | PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression |
| title_short | PD-L2 Expression in Breast Cancer Promotes Tumor Development and Progression |
| title_sort | pd l2 expression in breast cancer promotes tumor development and progression |
| url | http://dx.doi.org/10.1155/2024/3145695 |
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