Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical Features

Chronic liver diseases (CLDs) in dogs are progressive conditions that often lead to liver failure. Metabolic dysfunctions such as cholestasis, obesity, hyperlipidemia, and endocrine disorders play a key role in human liver diseases like MASLD (Metabolic Dysfunction Associated Steatotic Liver Disease...

Full description

Saved in:
Bibliographic Details
Main Authors: Verena Habermaass, Yuki Takami, Takeshi Izawa, Francesca Abramo, Corrado Biolatti, Veronica Marchetti
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Veterinary Sciences
Subjects:
Online Access:https://www.mdpi.com/2306-7381/12/3/220
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849339939494297600
author Verena Habermaass
Yuki Takami
Takeshi Izawa
Francesca Abramo
Corrado Biolatti
Veronica Marchetti
author_facet Verena Habermaass
Yuki Takami
Takeshi Izawa
Francesca Abramo
Corrado Biolatti
Veronica Marchetti
author_sort Verena Habermaass
collection DOAJ
description Chronic liver diseases (CLDs) in dogs are progressive conditions that often lead to liver failure. Metabolic dysfunctions such as cholestasis, obesity, hyperlipidemia, and endocrine disorders play a key role in human liver diseases like MASLD (Metabolic Dysfunction Associated Steatotic Liver Disease) and MASH (Metabolic Dysfunction Associated Steatohepatitis), but their significance in canine CLDs is poorly understood. This study aims to evaluate the association between hepatic lipid accumulation and inflammation or fibrosis in canine CLDs and its potential association with metabolic dysfunctions. Sixteen client-owned dogs with CLDs were assessed for clinical data, histological features, and liver immunohistochemistry (IHC). Histological and IHC markers of inflammation (Iba-1, iNOS, NF-κB), fibrosis (CD206, α-SMA, Sirius Red), and lipid accumulation (adipophilin) were assessed to identify correlations with clinical conditions. The applied markers showed effectiveness in their use on canine liver tissue. Adipophilin-marked lipid accumulation correlated positively with inflammatory markers, indicating a link between steatosis and inflammation. Metabolic dysfunctions were linked to hepatic lipid accumulation and inflammation. These findings show a potential alignment of canine CLDs with human MASLD/MASH, where lipid-induced inflammation drives disease progression. IHC markers could effectively assess these processes, suggesting potential for guiding diagnostics and therapies, though further research is needed to clarify clinical associations.
format Article
id doaj-art-91d26264da7e44c3946fba8a7b9d9a36
institution Kabale University
issn 2306-7381
language English
publishDate 2025-03-01
publisher MDPI AG
record_format Article
series Veterinary Sciences
spelling doaj-art-91d26264da7e44c3946fba8a7b9d9a362025-08-20T03:44:01ZengMDPI AGVeterinary Sciences2306-73812025-03-0112322010.3390/vetsci12030220Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical FeaturesVerena Habermaass0Yuki Takami1Takeshi Izawa2Francesca Abramo3Corrado Biolatti4Veronica Marchetti5Department of Veterinary Sciences, University of Pisa, Via Livornese Lato Monte, 56122 Pisa, ItalyLaboratory of Veterinary Pathology, Osaka Metropolitan University, 1-58 Rinku-Ourai-Kita, Izumisano-shi, Osaka 598-8531, JapanLaboratory of Veterinary Pathology, Osaka Metropolitan University, 1-58 Rinku-Ourai-Kita, Izumisano-shi, Osaka 598-8531, JapanDepartment of Veterinary Sciences, University of Pisa, Via Livornese Lato Monte, 56122 Pisa, ItalyIndependent Researcher, 6340 Baar, SwitzerlandDepartment of Veterinary Sciences, University of Pisa, Via Livornese Lato Monte, 56122 Pisa, ItalyChronic liver diseases (CLDs) in dogs are progressive conditions that often lead to liver failure. Metabolic dysfunctions such as cholestasis, obesity, hyperlipidemia, and endocrine disorders play a key role in human liver diseases like MASLD (Metabolic Dysfunction Associated Steatotic Liver Disease) and MASH (Metabolic Dysfunction Associated Steatohepatitis), but their significance in canine CLDs is poorly understood. This study aims to evaluate the association between hepatic lipid accumulation and inflammation or fibrosis in canine CLDs and its potential association with metabolic dysfunctions. Sixteen client-owned dogs with CLDs were assessed for clinical data, histological features, and liver immunohistochemistry (IHC). Histological and IHC markers of inflammation (Iba-1, iNOS, NF-κB), fibrosis (CD206, α-SMA, Sirius Red), and lipid accumulation (adipophilin) were assessed to identify correlations with clinical conditions. The applied markers showed effectiveness in their use on canine liver tissue. Adipophilin-marked lipid accumulation correlated positively with inflammatory markers, indicating a link between steatosis and inflammation. Metabolic dysfunctions were linked to hepatic lipid accumulation and inflammation. These findings show a potential alignment of canine CLDs with human MASLD/MASH, where lipid-induced inflammation drives disease progression. IHC markers could effectively assess these processes, suggesting potential for guiding diagnostics and therapies, though further research is needed to clarify clinical associations.https://www.mdpi.com/2306-7381/12/3/220lipidlivercholestasisobesitycanineimmunohistochemistry
spellingShingle Verena Habermaass
Yuki Takami
Takeshi Izawa
Francesca Abramo
Corrado Biolatti
Veronica Marchetti
Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical Features
Veterinary Sciences
lipid
liver
cholestasis
obesity
canine
immunohistochemistry
title Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical Features
title_full Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical Features
title_fullStr Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical Features
title_full_unstemmed Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical Features
title_short Lipid Dysmetabolism in Canine Chronic Liver Disease: Relationship Between Clinical, Histological and Immunohistochemical Features
title_sort lipid dysmetabolism in canine chronic liver disease relationship between clinical histological and immunohistochemical features
topic lipid
liver
cholestasis
obesity
canine
immunohistochemistry
url https://www.mdpi.com/2306-7381/12/3/220
work_keys_str_mv AT verenahabermaass lipiddysmetabolismincaninechronicliverdiseaserelationshipbetweenclinicalhistologicalandimmunohistochemicalfeatures
AT yukitakami lipiddysmetabolismincaninechronicliverdiseaserelationshipbetweenclinicalhistologicalandimmunohistochemicalfeatures
AT takeshiizawa lipiddysmetabolismincaninechronicliverdiseaserelationshipbetweenclinicalhistologicalandimmunohistochemicalfeatures
AT francescaabramo lipiddysmetabolismincaninechronicliverdiseaserelationshipbetweenclinicalhistologicalandimmunohistochemicalfeatures
AT corradobiolatti lipiddysmetabolismincaninechronicliverdiseaserelationshipbetweenclinicalhistologicalandimmunohistochemicalfeatures
AT veronicamarchetti lipiddysmetabolismincaninechronicliverdiseaserelationshipbetweenclinicalhistologicalandimmunohistochemicalfeatures