The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice
Limosilactobacillus reuteri QS01 is potential probiotic isolated from the intestinal microbiota of healthy women in early pregnancy. In the current study, we examined whether QS01 can prevent gestational diabetes mellitus (GDM) in an enhanced mouse model of the condition. Female C57BL/6J mouse offsp...
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Tsinghua University Press
2025-05-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250112 |
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| author | Ce Qi Haowen Zhang Zhenli Liu Xiaolin Liu Lintao Zhang Liangliang You Jin Sun |
| author_facet | Ce Qi Haowen Zhang Zhenli Liu Xiaolin Liu Lintao Zhang Liangliang You Jin Sun |
| author_sort | Ce Qi |
| collection | DOAJ |
| description | Limosilactobacillus reuteri QS01 is potential probiotic isolated from the intestinal microbiota of healthy women in early pregnancy. In the current study, we examined whether QS01 can prevent gestational diabetes mellitus (GDM) in an enhanced mouse model of the condition. Female C57BL/6J mouse offspring (F1 generation) born to dams fed a control (CON) or low-protein diet (GDM group) during gestation and lactation were used. Pregnant F1 mice fed a standard diet were randomly assigned to 5 groups: CON, GDM, and GDM mice treated with metformin, QS01 or Lacticaseibacillus rhamnosus HN001. An oral glucose tolerance test was performed before sacrifice at gestational day 17. Glucose tolerance was significantly ameliorated by all 3 treatments. QS01 supplementation fortified the intestinal mucosal barrier and inhibited the escalation of plasma inflammatory cytokines. QS01 treatment altered the cecal microbiota composition and function. Plasma and cecal metabolite profiles were modulated by QS01, prominently demonstrating significant upregulation of indole lactate and L-tryptophan in plasma and 5-hydroxy-tryptophan in the cecum, positively correlated with gut Lactobacillus abundance. In summary, QS01 plays a role in preventing GDM by remodeling the intestinal microbiota, reinforcing the intestinal mucosal barrier and alleviating chronic inflammation. |
| format | Article |
| id | doaj-art-91c5a222727044dea8262b1c5df5e280 |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-91c5a222727044dea8262b1c5df5e2802025-08-20T03:48:14ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-05-01145925011210.26599/FSHW.2024.9250112The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in miceCe Qi0Haowen Zhang1Zhenli Liu2Xiaolin Liu3Lintao Zhang4Liangliang You5Jin Sun6Institute of Nutrition and Health, Qingdao University, Qingdao 266023, ChinaInstitute of Nutrition and Health, Qingdao University, Qingdao 266023, ChinaTaian Maternal and Child Health Hospital, Tai’an 271025, ChinaTaian Maternal and Child Health Hospital, Tai’an 271025, ChinaJiaozhou Maternity and Child Health and Family Planning Service Center, Qingdao 266308, ChinaQingdao Newhope Qinpai Dairy Co., Ltd., Qingdao 266399, ChinaInstitute of Nutrition and Health, Qingdao University, Qingdao 266023, ChinaLimosilactobacillus reuteri QS01 is potential probiotic isolated from the intestinal microbiota of healthy women in early pregnancy. In the current study, we examined whether QS01 can prevent gestational diabetes mellitus (GDM) in an enhanced mouse model of the condition. Female C57BL/6J mouse offspring (F1 generation) born to dams fed a control (CON) or low-protein diet (GDM group) during gestation and lactation were used. Pregnant F1 mice fed a standard diet were randomly assigned to 5 groups: CON, GDM, and GDM mice treated with metformin, QS01 or Lacticaseibacillus rhamnosus HN001. An oral glucose tolerance test was performed before sacrifice at gestational day 17. Glucose tolerance was significantly ameliorated by all 3 treatments. QS01 supplementation fortified the intestinal mucosal barrier and inhibited the escalation of plasma inflammatory cytokines. QS01 treatment altered the cecal microbiota composition and function. Plasma and cecal metabolite profiles were modulated by QS01, prominently demonstrating significant upregulation of indole lactate and L-tryptophan in plasma and 5-hydroxy-tryptophan in the cecum, positively correlated with gut Lactobacillus abundance. In summary, QS01 plays a role in preventing GDM by remodeling the intestinal microbiota, reinforcing the intestinal mucosal barrier and alleviating chronic inflammation.https://www.sciopen.com/article/10.26599/FSHW.2024.9250112estational diabetes mellituslimosilactobacillus reuterigut microbiotamucosal barriertryptophan metabolism |
| spellingShingle | Ce Qi Haowen Zhang Zhenli Liu Xiaolin Liu Lintao Zhang Liangliang You Jin Sun The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice Food Science and Human Wellness estational diabetes mellitus limosilactobacillus reuteri gut microbiota mucosal barrier tryptophan metabolism |
| title | The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice |
| title_full | The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice |
| title_fullStr | The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice |
| title_full_unstemmed | The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice |
| title_short | The protective effect of Limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice |
| title_sort | protective effect of limosilactobacillus reuteri against gestational diabetes mellitus through restoring intestinal microbiota homeostasis and attenuating inflammation in mice |
| topic | estational diabetes mellitus limosilactobacillus reuteri gut microbiota mucosal barrier tryptophan metabolism |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250112 |
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