Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1
Abstract Background Cerebral ischemic disease is a common cerebrovascular disease, especially ischemic stroke. Exercise has protective functions on brain tissues following cerebral ischemia‐reperfusion injury (CIRI), but its preventive effects and mechanisms in CIRI remain unclear. We aimed to inves...
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Wiley
2024-10-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.70008 |
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| author | Xiangbo Meng Hui Yang Feifeng Chen Baohua Li Yan Wu Rong Wang |
| author_facet | Xiangbo Meng Hui Yang Feifeng Chen Baohua Li Yan Wu Rong Wang |
| author_sort | Xiangbo Meng |
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| description | Abstract Background Cerebral ischemic disease is a common cerebrovascular disease, especially ischemic stroke. Exercise has protective functions on brain tissues following cerebral ischemia‐reperfusion injury (CIRI), but its preventive effects and mechanisms in CIRI remain unclear. We aimed to investigate the effects and mechanisms of exercise preconditioning on CIRI. Methods The middle cerebral artery occlusion (MCAO) operation was prepared to establish CIRI rats. All rats were randomized into the MCAO, exercise (exercise preconditioning plus MCAO operation), vector (exercise preconditioning, MCAO operation plus intraventricular injection of empty vector), and tissue inhibitor of metalloprotease 1 overexpression (OE‐TIMP1, exercise preconditioning, MCAO operation plus intraventricular injection of OE‐TIMP1) groups. Results The results indicated that exercise preconditioning suppressed approximately 66.67% of neurological deficit scores and 73.79% of TIMP1 mRNA expression in MCAO rats, which were partially offset by OE‐TIMP1. The protective effects of exercise against neuron death status and cerebral infarction size in MCAO rats were reversed by OE‐TIMP1. It also confirmed that exercise weakened apoptosis and oxidative stress damage, with notable increases of B‐cell lymphoma‐2, superoxide dismutase, and glutathione peroxidase production, and evident decreases of BCL2‐associated X, caspase 3, and malondialdehyde in MCAO rats, while these effects were partially reversed by OE‐TIMP1. Additionally, the inhibitory effects of exercise on the protein levels of TIMP1, hypoxia‐inducible factor‐alpha, vascular endothelial growth factor receptor 2, vascular endothelial growth factor, and neurogenic locus notch homolog protein 1 in MCAO rats were partially reversed by OE‐TIMP1. Conclusion Altogether, exercise preconditioning had protective effects on CIRI by restraining TIMP1, which provided new therapeutic strategies for preventing CIRI. |
| format | Article |
| id | doaj-art-91b2a1e3aa494cf79b6fd2f7f5224094 |
| institution | OA Journals |
| issn | 2050-4527 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
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| series | Immunity, Inflammation and Disease |
| spelling | doaj-art-91b2a1e3aa494cf79b6fd2f7f52240942025-08-20T02:12:10ZengWileyImmunity, Inflammation and Disease2050-45272024-10-011210n/an/a10.1002/iid3.70008Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1Xiangbo Meng0Hui Yang1Feifeng Chen2Baohua Li3Yan Wu4Rong Wang5Department of Rehabilitation Medicine The Affiliated Hospital of Hangzhou Normal University Hangzhou 310000 Zhejiang Province ChinaDepartment of Neurology Hangzhou First People's Hospital Hangzhou 310006 Zhejiang Province ChinaDepartment of Rehabilitation Medicine Hangzhou First People's Hospital Hangzhou 310006 Zhejiang Province ChinaDepartment of Neurology Hangzhou First People's Hospital Hangzhou 310006 Zhejiang Province ChinaDepartment of Rehabilitation Medicine Hangzhou First People's Hospital Hangzhou 310006 Zhejiang Province ChinaDepartment of Radiology Hangzhou First People's Hospital Hangzhou 310006 Zhejiang Province ChinaAbstract Background Cerebral ischemic disease is a common cerebrovascular disease, especially ischemic stroke. Exercise has protective functions on brain tissues following cerebral ischemia‐reperfusion injury (CIRI), but its preventive effects and mechanisms in CIRI remain unclear. We aimed to investigate the effects and mechanisms of exercise preconditioning on CIRI. Methods The middle cerebral artery occlusion (MCAO) operation was prepared to establish CIRI rats. All rats were randomized into the MCAO, exercise (exercise preconditioning plus MCAO operation), vector (exercise preconditioning, MCAO operation plus intraventricular injection of empty vector), and tissue inhibitor of metalloprotease 1 overexpression (OE‐TIMP1, exercise preconditioning, MCAO operation plus intraventricular injection of OE‐TIMP1) groups. Results The results indicated that exercise preconditioning suppressed approximately 66.67% of neurological deficit scores and 73.79% of TIMP1 mRNA expression in MCAO rats, which were partially offset by OE‐TIMP1. The protective effects of exercise against neuron death status and cerebral infarction size in MCAO rats were reversed by OE‐TIMP1. It also confirmed that exercise weakened apoptosis and oxidative stress damage, with notable increases of B‐cell lymphoma‐2, superoxide dismutase, and glutathione peroxidase production, and evident decreases of BCL2‐associated X, caspase 3, and malondialdehyde in MCAO rats, while these effects were partially reversed by OE‐TIMP1. Additionally, the inhibitory effects of exercise on the protein levels of TIMP1, hypoxia‐inducible factor‐alpha, vascular endothelial growth factor receptor 2, vascular endothelial growth factor, and neurogenic locus notch homolog protein 1 in MCAO rats were partially reversed by OE‐TIMP1. Conclusion Altogether, exercise preconditioning had protective effects on CIRI by restraining TIMP1, which provided new therapeutic strategies for preventing CIRI.https://doi.org/10.1002/iid3.70008brain injurycerebral ischemia‐reperfusion injuryexercise preconditioningTIMP1 |
| spellingShingle | Xiangbo Meng Hui Yang Feifeng Chen Baohua Li Yan Wu Rong Wang Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1 Immunity, Inflammation and Disease brain injury cerebral ischemia‐reperfusion injury exercise preconditioning TIMP1 |
| title | Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1 |
| title_full | Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1 |
| title_fullStr | Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1 |
| title_full_unstemmed | Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1 |
| title_short | Exercise preconditioning mitigates brain injury after cerebral ischemia‐reperfusion injury in rats by restraining TIMP1 |
| title_sort | exercise preconditioning mitigates brain injury after cerebral ischemia reperfusion injury in rats by restraining timp1 |
| topic | brain injury cerebral ischemia‐reperfusion injury exercise preconditioning TIMP1 |
| url | https://doi.org/10.1002/iid3.70008 |
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