Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1
The complemental deoxyribonucleic acid (cDNA) of Bombyx mandarina prophenoloxidase gene PPO1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 2 086 bp in length contained an open reading frame (ORF) of 2 058 bp which encoded 685 amino acid...
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Zhejiang University Press
2012-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.004 |
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| author | ZHANG Yong-liang SHENG Dong-feng ZHU Yong |
| author_facet | ZHANG Yong-liang SHENG Dong-feng ZHU Yong |
| author_sort | ZHANG Yong-liang |
| collection | DOAJ |
| description | The complemental deoxyribonucleic acid (cDNA) of Bombyx mandarina prophenoloxidase gene PPO1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 2 086 bp in length contained an open reading frame (ORF) of 2 058 bp which encoded 685 amino acid residues. The deduced amino acid sequence had a high identity to the reported sequence of PPO1 from other lepidopterous insects and shared the typical structural features of PPO from other insects. The PPO1 gene was only expressed in head and blood of B. mandarina by RT-PCR, and only expressed in blood by Northern blot analysis. These results provide a molecular basis for further studying the function of PPO1 gene in B. mandarina. |
| format | Article |
| id | doaj-art-919945944d5f4a23b9569a355710a30d |
| institution | Kabale University |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2012-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-919945944d5f4a23b9569a355710a30d2025-08-20T03:58:18ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552012-05-013825626210.3785/j.issn.1008-9209.2012.03.00410089209Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1ZHANG Yong-liangSHENG Dong-fengZHU YongThe complemental deoxyribonucleic acid (cDNA) of Bombyx mandarina prophenoloxidase gene PPO1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR). The results showed that the cDNA with 2 086 bp in length contained an open reading frame (ORF) of 2 058 bp which encoded 685 amino acid residues. The deduced amino acid sequence had a high identity to the reported sequence of PPO1 from other lepidopterous insects and shared the typical structural features of PPO from other insects. The PPO1 gene was only expressed in head and blood of B. mandarina by RT-PCR, and only expressed in blood by Northern blot analysis. These results provide a molecular basis for further studying the function of PPO1 gene in B. mandarina.https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.004<italic>Bombyx mandarina</italic>prophenoloxidase gene <italic>PPO1</italic>cloningsequence analysisgene expression |
| spellingShingle | ZHANG Yong-liang SHENG Dong-feng ZHU Yong Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1 浙江大学学报. 农业与生命科学版 <italic>Bombyx mandarina</italic> prophenoloxidase gene <italic>PPO1</italic> cloning sequence analysis gene expression |
| title | Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1 |
| title_full | Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1 |
| title_fullStr | Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1 |
| title_full_unstemmed | Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1 |
| title_short | Cloning and characterization of Bombyx mandarina prophenoloxidase gene PPO1 |
| title_sort | cloning and characterization of bombyx mandarina prophenoloxidase gene ppo1 |
| topic | <italic>Bombyx mandarina</italic> prophenoloxidase gene <italic>PPO1</italic> cloning sequence analysis gene expression |
| url | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.03.004 |
| work_keys_str_mv | AT zhangyongliang cloningandcharacterizationofbombyxmandarinaprophenoloxidasegeneppo1 AT shengdongfeng cloningandcharacterizationofbombyxmandarinaprophenoloxidasegeneppo1 AT zhuyong cloningandcharacterizationofbombyxmandarinaprophenoloxidasegeneppo1 |