The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae)
The present study has aimed to examine the effects of olive leaf extract on the antioxidant enzyme activity of the invertebrate model organism Galleria mellonella L. (Lepidoptera: Pyralidae) hemolymph. For this purpose, after the olive leaves collected from Çanakkale province were dried at room temp...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tokat Gaziosmanpasa University
2023-12-01
|
| Series: | Journal of New Results in Science |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/en/download/article-file/3567946 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849338660074291200 |
|---|---|
| author | Tuğba Söküt Açar Seranay Türkdoğan Serhat Kaya |
| author_facet | Tuğba Söküt Açar Seranay Türkdoğan Serhat Kaya |
| author_sort | Tuğba Söküt Açar |
| collection | DOAJ |
| description | The present study has aimed to examine the effects of olive leaf extract on the antioxidant enzyme activity of the invertebrate model organism Galleria mellonella L. (Lepidoptera: Pyralidae) hemolymph. For this purpose, after the olive leaves collected from Çanakkale province were dried at room temperature, the ethanolic extract was obtained with a soxhlet. After the ethanol was removed from the obtained extract in the rotary evaporator, the obtained dry matter was prepared in the phosphate buffer salt, 72 mg mL-1 as the highest dose and the lowest 0.010 mg mL-1 and injected into the larvae reared for the experiment. Hemolymph was collected at 4, 8, 12, 16, and 24-hours post-injection. Catalase (CAT) and superoxide dismutase (SOD) enzyme activities and malondialdehyde (MDA) formation levels were determined spectrophotometrically in the collected hemolymph. According to the results obtained, olive leaf extract decreased CAT and SOD activity in G. mellonella hemolymph at certain doses and increased the amount of MDA. For all enzymes, group, dose, and group-dose interactions were statistically significant. In addition, as a result of the examination made in terms of time, it was seen that the measurement of enzyme activities at the 24th hour was significant. The results of our study show that the antioxidant capacity of the olive leaf cannot be transferred to other living things and even reduces the antioxidant capacity of other organisms. |
| format | Article |
| id | doaj-art-91983febb4e245b8a74df6d1825af3d6 |
| institution | Kabale University |
| issn | 1304-7981 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Tokat Gaziosmanpasa University |
| record_format | Article |
| series | Journal of New Results in Science |
| spelling | doaj-art-91983febb4e245b8a74df6d1825af3d62025-08-20T03:44:20ZengTokat Gaziosmanpasa UniversityJournal of New Results in Science1304-79812023-12-0112318820010.54187/jnrs.1397882142The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae)Tuğba Söküt Açar0https://orcid.org/0000-0002-4444-1671Seranay Türkdoğan1https://orcid.org/0000-0001-8827-6631Serhat Kaya2https://orcid.org/0000-0002-0984-2824CANAKKALE ONSEKIZ MART UNIVERSITY, FACULTY OF ARTS AND SCIENCESBALIKESİR ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜÇANAKKALE ONSEKİZ MART ÜNİVERSİTESİ, FEN VE EDEBİYAT FAKÜLTESİ, BİYOLOJİ BÖLÜMÜThe present study has aimed to examine the effects of olive leaf extract on the antioxidant enzyme activity of the invertebrate model organism Galleria mellonella L. (Lepidoptera: Pyralidae) hemolymph. For this purpose, after the olive leaves collected from Çanakkale province were dried at room temperature, the ethanolic extract was obtained with a soxhlet. After the ethanol was removed from the obtained extract in the rotary evaporator, the obtained dry matter was prepared in the phosphate buffer salt, 72 mg mL-1 as the highest dose and the lowest 0.010 mg mL-1 and injected into the larvae reared for the experiment. Hemolymph was collected at 4, 8, 12, 16, and 24-hours post-injection. Catalase (CAT) and superoxide dismutase (SOD) enzyme activities and malondialdehyde (MDA) formation levels were determined spectrophotometrically in the collected hemolymph. According to the results obtained, olive leaf extract decreased CAT and SOD activity in G. mellonella hemolymph at certain doses and increased the amount of MDA. For all enzymes, group, dose, and group-dose interactions were statistically significant. In addition, as a result of the examination made in terms of time, it was seen that the measurement of enzyme activities at the 24th hour was significant. The results of our study show that the antioxidant capacity of the olive leaf cannot be transferred to other living things and even reduces the antioxidant capacity of other organisms.https://dergipark.org.tr/en/download/article-file/3567946olea europaeagalleria mellonellacatalasesuperoxide dismutasemalondialdehyde |
| spellingShingle | Tuğba Söküt Açar Seranay Türkdoğan Serhat Kaya The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae) Journal of New Results in Science olea europaea galleria mellonella catalase superoxide dismutase malondialdehyde |
| title | The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae) |
| title_full | The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae) |
| title_fullStr | The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae) |
| title_full_unstemmed | The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae) |
| title_short | The influence of olive leaf extract on the activity of antioxidant enzymes in the Hemolymph of Galleria mellonella (L.) (Lepidoptera: Pyralidae) |
| title_sort | influence of olive leaf extract on the activity of antioxidant enzymes in the hemolymph of galleria mellonella l lepidoptera pyralidae |
| topic | olea europaea galleria mellonella catalase superoxide dismutase malondialdehyde |
| url | https://dergipark.org.tr/en/download/article-file/3567946 |
| work_keys_str_mv | AT tugbasokutacar theinfluenceofoliveleafextractontheactivityofantioxidantenzymesinthehemolymphofgalleriamellonellallepidopterapyralidae AT seranayturkdogan theinfluenceofoliveleafextractontheactivityofantioxidantenzymesinthehemolymphofgalleriamellonellallepidopterapyralidae AT serhatkaya theinfluenceofoliveleafextractontheactivityofantioxidantenzymesinthehemolymphofgalleriamellonellallepidopterapyralidae AT tugbasokutacar influenceofoliveleafextractontheactivityofantioxidantenzymesinthehemolymphofgalleriamellonellallepidopterapyralidae AT seranayturkdogan influenceofoliveleafextractontheactivityofantioxidantenzymesinthehemolymphofgalleriamellonellallepidopterapyralidae AT serhatkaya influenceofoliveleafextractontheactivityofantioxidantenzymesinthehemolymphofgalleriamellonellallepidopterapyralidae |