CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1
Abstract Background Nasopharyngeal carcinoma (NPC) is a malignant head and neck cancer with high mortality and dismal prognosis. Emerging research have disclosed that circRNAs are crucial gene expression regulators engaged in tumor advancement. This work aspired to identify novel oncogenic circRNA d...
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| Language: | English |
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BMC
2025-03-01
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| Series: | Hereditas |
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| Online Access: | https://doi.org/10.1186/s41065-025-00406-7 |
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| author | Rui Wang Fei Wang |
| author_facet | Rui Wang Fei Wang |
| author_sort | Rui Wang |
| collection | DOAJ |
| description | Abstract Background Nasopharyngeal carcinoma (NPC) is a malignant head and neck cancer with high mortality and dismal prognosis. Emerging research have disclosed that circRNAs are crucial gene expression regulators engaged in tumor advancement. This work aspired to identify novel oncogenic circRNA driving NPC progression. Methods Bioinformatics analysis was performed to explore and predict underlying circRNA and downstream targets. Luciferase reporter assay was executed to check the binding relationship between these genes. Cell function tests were conducted using CCK-8, would healing, and flow cytometry. The stemness markers CD133, Nanog and Oct4 was detected via western blot. Results CircCENPM was notably enhanced in NPC. Silencing of circCENPM suppressed NPC cell growth, migration, and stemness in vitro, simultaneously impeded tumorigenesis of NPC in vivo. Moreover, circCENPM could interact with miR-362-3p, whereas miR-362-3p inhibitor apparently reversed the mitigated growth and stemness induced by circCENPM knockdown in NPC cells. Furthermore, BMI1 was identified to be the downstream target of miR-362-3p, and BMI1 introduction partially offset the anti-tumor function of miR-362-3p in NPC cells. Conclusion CircCENPM functioned as a carcinogenic driver and facilitated NPC growth and stemness via miR-362-3p/BMI1 regulatory network, which provided a potential biomarker and attractive target for NPC intervention and treatment. |
| format | Article |
| id | doaj-art-9183b7e01a1d4f43a4fe570aa29d77dc |
| institution | DOAJ |
| issn | 1601-5223 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Hereditas |
| spelling | doaj-art-9183b7e01a1d4f43a4fe570aa29d77dc2025-08-20T02:56:09ZengBMCHereditas1601-52232025-03-01162111310.1186/s41065-025-00406-7CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1Rui Wang0Fei Wang1The Second Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Kunming Medical UniversityDepartment of Ultrasound, The First Affiliated Hospital of Kunming Medical UniversityAbstract Background Nasopharyngeal carcinoma (NPC) is a malignant head and neck cancer with high mortality and dismal prognosis. Emerging research have disclosed that circRNAs are crucial gene expression regulators engaged in tumor advancement. This work aspired to identify novel oncogenic circRNA driving NPC progression. Methods Bioinformatics analysis was performed to explore and predict underlying circRNA and downstream targets. Luciferase reporter assay was executed to check the binding relationship between these genes. Cell function tests were conducted using CCK-8, would healing, and flow cytometry. The stemness markers CD133, Nanog and Oct4 was detected via western blot. Results CircCENPM was notably enhanced in NPC. Silencing of circCENPM suppressed NPC cell growth, migration, and stemness in vitro, simultaneously impeded tumorigenesis of NPC in vivo. Moreover, circCENPM could interact with miR-362-3p, whereas miR-362-3p inhibitor apparently reversed the mitigated growth and stemness induced by circCENPM knockdown in NPC cells. Furthermore, BMI1 was identified to be the downstream target of miR-362-3p, and BMI1 introduction partially offset the anti-tumor function of miR-362-3p in NPC cells. Conclusion CircCENPM functioned as a carcinogenic driver and facilitated NPC growth and stemness via miR-362-3p/BMI1 regulatory network, which provided a potential biomarker and attractive target for NPC intervention and treatment.https://doi.org/10.1186/s41065-025-00406-7NPCCircCENPMmiR-362-3pBMI1Metastasis |
| spellingShingle | Rui Wang Fei Wang CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1 Hereditas NPC CircCENPM miR-362-3p BMI1 Metastasis |
| title | CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1 |
| title_full | CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1 |
| title_fullStr | CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1 |
| title_full_unstemmed | CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1 |
| title_short | CircCENPM serves as a CeRNA to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing BMI1 |
| title_sort | circcenpm serves as a cerna to aggravate nasopharyngeal carcinoma metastasis and stemness via enhancing bmi1 |
| topic | NPC CircCENPM miR-362-3p BMI1 Metastasis |
| url | https://doi.org/10.1186/s41065-025-00406-7 |
| work_keys_str_mv | AT ruiwang circcenpmservesasacernatoaggravatenasopharyngealcarcinomametastasisandstemnessviaenhancingbmi1 AT feiwang circcenpmservesasacernatoaggravatenasopharyngealcarcinomametastasisandstemnessviaenhancingbmi1 |