From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i>
<b>Background:</b> Varicose veins and chronic venous insufficiency are chronic venous disorders involving abnormalities in the venous system. Inflammation, an increase in proteolytic enzymes, and free radicals are important factors that play a role in the varicose vein pathology. <b&g...
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2025-06-01
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| author | Tugsen Buyukyildirim F. Sezer Senol Deniz Merve Yuzbasioglu Baran Ece Salihoglu Mustafa Abdullah Yilmaz Osman Tugay |
| author_facet | Tugsen Buyukyildirim F. Sezer Senol Deniz Merve Yuzbasioglu Baran Ece Salihoglu Mustafa Abdullah Yilmaz Osman Tugay |
| author_sort | Tugsen Buyukyildirim |
| collection | DOAJ |
| description | <b>Background:</b> Varicose veins and chronic venous insufficiency are chronic venous disorders involving abnormalities in the venous system. Inflammation, an increase in proteolytic enzymes, and free radicals are important factors that play a role in the varicose vein pathology. <b>Methods:</b> In this study, the antioxidant properties and inhibitor activities of 17 plant extracts used to treat varicose veins in traditional medicine were evaluated against varicose veins-related enzymes (hyaluronidase, elastase, collagenase, lipooxygenase, prolylendopeptidase, and xanthine oxidase). The most effective compounds responsible for the activity of the <i>Helichrysum plicatum</i> subsp. <i>plicatum</i> extract were isolated by open column chromatography techniques. The active compounds were determined to be naringenin, apigenin, and luteolin by spectroscopic methods. In the activity-guided isolation study, the xanthine oxidase enzyme inhibition method was used. <b>Results:</b> The fractions containing naringenin and apigenin (IC<sub>50</sub> = 0.269 ± 0.009 µg/mL) and apigenin and luteolin (IC<sub>50</sub> = 0.285 ± 0.019 µg/mL) compounds showed synergistic and strong effects against xanthine oxidase and were found to be as active as the positive control allopurinol (IC<sub>50</sub> = 0.250 ± 0.006 µg/mL). In the LC-MS/MS analysis of the <i>Helichrysum plicatum</i> extract, quinic acid (22.649 mg compound/g extract), chlorogenic acid (14.573 mg/g extract), isoquercitrin (14.371 mg/g extract), cosmosin (9.885 mg/g extract), and astragalin (11.506 mg/g extract) were detected as the major components. Naringenin, apigenin, and luteolin were detected at concentrations of 1.457, 2.518, and 1.368 mg/g in the extract, respectively. <b>Conclusions:</b> In conclusion, it is predicted that the combination of naringenin, apigenin, and luteolin has a promising use as a conservative treatment option for diseases associated with varicose veins due to their synergistic effects with each other. |
| format | Article |
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| institution | Kabale University |
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| spelling | doaj-art-9183389fac8b42a5b0e1dee5a635c7bc2025-08-20T03:29:45ZengMDPI AGPharmaceuticals1424-82472025-06-0118692610.3390/ph18060926From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i>Tugsen Buyukyildirim0F. Sezer Senol Deniz1Merve Yuzbasioglu Baran2Ece Salihoglu3Mustafa Abdullah Yilmaz4Osman Tugay5Department of Pharmacognosy, Faculty of Pharmacy, Selcuk University, 42071 Konya, TürkiyeDepartment of Pharmacognosy, Faculty of Pharmacy, Gazi University, 06330 Ankara, TürkiyeDepartment of Pharmacognosy, Gülhane Faculty of Pharmacy, University of Health Sciences, 06108 Ankara, TürkiyeDepartment of Biochemistry, Faculty of Pharmacy, Gazi University, 06330 Ankara, TürkiyeScience and Technology Research and Application Center, Dicle University, 21280 Diyarbakır, TürkiyeDepartment of Pharmaceutical Botany, Faculty of Pharmacy, Selcuk University, 42071 Konya, Türkiye<b>Background:</b> Varicose veins and chronic venous insufficiency are chronic venous disorders involving abnormalities in the venous system. Inflammation, an increase in proteolytic enzymes, and free radicals are important factors that play a role in the varicose vein pathology. <b>Methods:</b> In this study, the antioxidant properties and inhibitor activities of 17 plant extracts used to treat varicose veins in traditional medicine were evaluated against varicose veins-related enzymes (hyaluronidase, elastase, collagenase, lipooxygenase, prolylendopeptidase, and xanthine oxidase). The most effective compounds responsible for the activity of the <i>Helichrysum plicatum</i> subsp. <i>plicatum</i> extract were isolated by open column chromatography techniques. The active compounds were determined to be naringenin, apigenin, and luteolin by spectroscopic methods. In the activity-guided isolation study, the xanthine oxidase enzyme inhibition method was used. <b>Results:</b> The fractions containing naringenin and apigenin (IC<sub>50</sub> = 0.269 ± 0.009 µg/mL) and apigenin and luteolin (IC<sub>50</sub> = 0.285 ± 0.019 µg/mL) compounds showed synergistic and strong effects against xanthine oxidase and were found to be as active as the positive control allopurinol (IC<sub>50</sub> = 0.250 ± 0.006 µg/mL). In the LC-MS/MS analysis of the <i>Helichrysum plicatum</i> extract, quinic acid (22.649 mg compound/g extract), chlorogenic acid (14.573 mg/g extract), isoquercitrin (14.371 mg/g extract), cosmosin (9.885 mg/g extract), and astragalin (11.506 mg/g extract) were detected as the major components. Naringenin, apigenin, and luteolin were detected at concentrations of 1.457, 2.518, and 1.368 mg/g in the extract, respectively. <b>Conclusions:</b> In conclusion, it is predicted that the combination of naringenin, apigenin, and luteolin has a promising use as a conservative treatment option for diseases associated with varicose veins due to their synergistic effects with each other.https://www.mdpi.com/1424-8247/18/6/926<i>Helichrysum plicatum</i> subsp. <i>plicatum</i>varicose veinin vitro enzyme inhibition activityantioxidant activityflavonoidsnaringenin |
| spellingShingle | Tugsen Buyukyildirim F. Sezer Senol Deniz Merve Yuzbasioglu Baran Ece Salihoglu Mustafa Abdullah Yilmaz Osman Tugay From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i> Pharmaceuticals <i>Helichrysum plicatum</i> subsp. <i>plicatum</i> varicose vein in vitro enzyme inhibition activity antioxidant activity flavonoids naringenin |
| title | From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i> |
| title_full | From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i> |
| title_fullStr | From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i> |
| title_full_unstemmed | From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i> |
| title_short | From Ethnopharmacology to Active Compound: Effects of Traditional Plant Extracts on Varicose Vein-Related Enzymes and Isolation of Active Flavonoids from <i>Helichrysum plicatum</i> DC. subsp. <i>plicatum</i> |
| title_sort | from ethnopharmacology to active compound effects of traditional plant extracts on varicose vein related enzymes and isolation of active flavonoids from i helichrysum plicatum i dc subsp i plicatum i |
| topic | <i>Helichrysum plicatum</i> subsp. <i>plicatum</i> varicose vein in vitro enzyme inhibition activity antioxidant activity flavonoids naringenin |
| url | https://www.mdpi.com/1424-8247/18/6/926 |
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