Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro Study
Background. The application of herbal and/or chemical antimicrobial mouthwashes in addition to the mechanical methods of bacteria removal helps reduce the periopathogens and thus increase the periodontal tissues’ health. The present study aimed to evaluate the antibacterial effect of Thymex (TMX) sy...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2022/6359841 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849695618942894080 |
|---|---|
| author | Mohammad Mahdi Yaghooti Khorasani Yasaman Mohammadi Kamalabadi Somaye Salari Sedigh Mina Jafari Mostafa Sadeghi Sahar Assar |
| author_facet | Mohammad Mahdi Yaghooti Khorasani Yasaman Mohammadi Kamalabadi Somaye Salari Sedigh Mina Jafari Mostafa Sadeghi Sahar Assar |
| author_sort | Mohammad Mahdi Yaghooti Khorasani |
| collection | DOAJ |
| description | Background. The application of herbal and/or chemical antimicrobial mouthwashes in addition to the mechanical methods of bacteria removal helps reduce the periopathogens and thus increase the periodontal tissues’ health. The present study aimed to evaluate the antibacterial effect of Thymex (TMX) syrup on three periodontal facultative anaerobes in vitro and compare it with 0.2% chlorhexidine (CHX) mouthwash. Methods. In this in vitro experiment, the disc diffusion method was used to measure the inhibitory halo diameter (IhD) of Enterobacter cloacae, Actinomyces viscosus, and Eikenella corrodens. The paper discs containing TMX and CHX were placed on Mueller–Hinton agar media and cultured with the mentioned bacteria. Moreover, a blank disc containing distilled water was used as a control. From each of the three bacterial species, five samples were taken, and after 18 hours of storage in the incubator, the IhDs were measured in millimeters. A one-way ANOVA test and an independent sample t-test were used to compare the mean differences of IhDs between groups. The significance level was considered to be 0.05. Results. The IhDs ranged between 6.2–8.8 mm and 12.3–34 mm for TMX and CHX, respectively. CHX showed a more inhibitory effect on all three species of bacteria compared to TMX mouthwash (P<0.001). Conclusions. Despite the inhibitory effect of TMX on bacterial growth, CHX showed significantly more antibacterial activity than TMX against three studied bacterial species. |
| format | Article |
| id | doaj-art-917b989018564aea815f48401bbafd28 |
| institution | DOAJ |
| issn | 1687-8736 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-917b989018564aea815f48401bbafd282025-08-20T03:19:42ZengWileyInternational Journal of Dentistry1687-87362022-01-01202210.1155/2022/6359841Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro StudyMohammad Mahdi Yaghooti Khorasani0Yasaman Mohammadi Kamalabadi1Somaye Salari Sedigh2Mina Jafari3Mostafa Sadeghi4Sahar Assar5Department of EndodonticsUniversity of Western OntarioDepartment of PeriodonticsSchool of DentistryDepartment of Operative DentistrySchool of DentistryBackground. The application of herbal and/or chemical antimicrobial mouthwashes in addition to the mechanical methods of bacteria removal helps reduce the periopathogens and thus increase the periodontal tissues’ health. The present study aimed to evaluate the antibacterial effect of Thymex (TMX) syrup on three periodontal facultative anaerobes in vitro and compare it with 0.2% chlorhexidine (CHX) mouthwash. Methods. In this in vitro experiment, the disc diffusion method was used to measure the inhibitory halo diameter (IhD) of Enterobacter cloacae, Actinomyces viscosus, and Eikenella corrodens. The paper discs containing TMX and CHX were placed on Mueller–Hinton agar media and cultured with the mentioned bacteria. Moreover, a blank disc containing distilled water was used as a control. From each of the three bacterial species, five samples were taken, and after 18 hours of storage in the incubator, the IhDs were measured in millimeters. A one-way ANOVA test and an independent sample t-test were used to compare the mean differences of IhDs between groups. The significance level was considered to be 0.05. Results. The IhDs ranged between 6.2–8.8 mm and 12.3–34 mm for TMX and CHX, respectively. CHX showed a more inhibitory effect on all three species of bacteria compared to TMX mouthwash (P<0.001). Conclusions. Despite the inhibitory effect of TMX on bacterial growth, CHX showed significantly more antibacterial activity than TMX against three studied bacterial species.http://dx.doi.org/10.1155/2022/6359841 |
| spellingShingle | Mohammad Mahdi Yaghooti Khorasani Yasaman Mohammadi Kamalabadi Somaye Salari Sedigh Mina Jafari Mostafa Sadeghi Sahar Assar Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro Study International Journal of Dentistry |
| title | Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro Study |
| title_full | Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro Study |
| title_fullStr | Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro Study |
| title_full_unstemmed | Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro Study |
| title_short | Comparative Evaluation of a Commercial Herbal Extract and 0.2% Chlorhexidine Mouthwash on Three Periodontal Facultative Anaerobes: An In Vitro Study |
| title_sort | comparative evaluation of a commercial herbal extract and 0 2 chlorhexidine mouthwash on three periodontal facultative anaerobes an in vitro study |
| url | http://dx.doi.org/10.1155/2022/6359841 |
| work_keys_str_mv | AT mohammadmahdiyaghootikhorasani comparativeevaluationofacommercialherbalextractand02chlorhexidinemouthwashonthreeperiodontalfacultativeanaerobesaninvitrostudy AT yasamanmohammadikamalabadi comparativeevaluationofacommercialherbalextractand02chlorhexidinemouthwashonthreeperiodontalfacultativeanaerobesaninvitrostudy AT somayesalarisedigh comparativeevaluationofacommercialherbalextractand02chlorhexidinemouthwashonthreeperiodontalfacultativeanaerobesaninvitrostudy AT minajafari comparativeevaluationofacommercialherbalextractand02chlorhexidinemouthwashonthreeperiodontalfacultativeanaerobesaninvitrostudy AT mostafasadeghi comparativeevaluationofacommercialherbalextractand02chlorhexidinemouthwashonthreeperiodontalfacultativeanaerobesaninvitrostudy AT saharassar comparativeevaluationofacommercialherbalextractand02chlorhexidinemouthwashonthreeperiodontalfacultativeanaerobesaninvitrostudy |