Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity
Nanoparticles as drug delivery systems offer benefits such as protection of the encapsulated drug against degradation, site-specific targeting and prolonged blood circulation times. The aim of this study was to investigate nanoparticle uptake into Caco-2 cell monolayers, their co-localization within...
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| Format: | Article |
| Language: | English |
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Budapest University of Technology and Economics
2014-03-01
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| Series: | eXPRESS Polymer Letters |
| Subjects: | |
| Online Access: | http://www.expresspolymlett.com/letolt.php?file=EPL-0004774&mi=cd |
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| author | J. H. Hamman L. A. Nkabinde L. N. N. Shoba-Zikhali B. Semete-Makokotlela L. Kalombo H. Swai A. Grobler |
| author_facet | J. H. Hamman L. A. Nkabinde L. N. N. Shoba-Zikhali B. Semete-Makokotlela L. Kalombo H. Swai A. Grobler |
| author_sort | J. H. Hamman |
| collection | DOAJ |
| description | Nanoparticles as drug delivery systems offer benefits such as protection of the encapsulated drug against degradation, site-specific targeting and prolonged blood circulation times. The aim of this study was to investigate nanoparticle uptake into Caco-2 cell monolayers, their co-localization within the lysosomal compartment and their cytotoxicity in different cell lines. Rhodamine-6G labelled poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles were prepared by a double emulsion solvent evaporation freeze-drying method. Uptake and co-localisation of PLGA nanoparticles in lysosomes were visualized by confocal laser scanning microscopy. The cytotoxicity of the nanoparticles was evaluated on different mammalian cells lines by means of Trypan blue exclusion and the MTS assay. The PLGA nanoparticles accumulated in the intercellular spaces of Caco-2 cell monolayers, but were also taken up transcellularly into the Caco-2 cells and partially co-localized within the lysosomal compartment indicating involvement of endocytosis during uptake. PLGA nanoparticles did not show cytotoxic effects in all three cell lines. Intact PLGA nanoparticles are therefore capable of moving across epithelial cell membranes partly by means of endocytosis without causing cytotoxic effects. |
| format | Article |
| id | doaj-art-916fdf46f7db4e70b2e33bd3bf17e65e |
| institution | Kabale University |
| issn | 1788-618X |
| language | English |
| publishDate | 2014-03-01 |
| publisher | Budapest University of Technology and Economics |
| record_format | Article |
| series | eXPRESS Polymer Letters |
| spelling | doaj-art-916fdf46f7db4e70b2e33bd3bf17e65e2025-08-20T03:36:58ZengBudapest University of Technology and EconomicseXPRESS Polymer Letters1788-618X2014-03-018319720610.3144/expresspolymlett.2014.23Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicityJ. H. HammanL. A. NkabindeL. N. N. Shoba-ZikhaliB. Semete-MakokotlelaL. KalomboH. SwaiA. GroblerNanoparticles as drug delivery systems offer benefits such as protection of the encapsulated drug against degradation, site-specific targeting and prolonged blood circulation times. The aim of this study was to investigate nanoparticle uptake into Caco-2 cell monolayers, their co-localization within the lysosomal compartment and their cytotoxicity in different cell lines. Rhodamine-6G labelled poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles were prepared by a double emulsion solvent evaporation freeze-drying method. Uptake and co-localisation of PLGA nanoparticles in lysosomes were visualized by confocal laser scanning microscopy. The cytotoxicity of the nanoparticles was evaluated on different mammalian cells lines by means of Trypan blue exclusion and the MTS assay. The PLGA nanoparticles accumulated in the intercellular spaces of Caco-2 cell monolayers, but were also taken up transcellularly into the Caco-2 cells and partially co-localized within the lysosomal compartment indicating involvement of endocytosis during uptake. PLGA nanoparticles did not show cytotoxic effects in all three cell lines. Intact PLGA nanoparticles are therefore capable of moving across epithelial cell membranes partly by means of endocytosis without causing cytotoxic effects.http://www.expresspolymlett.com/letolt.php?file=EPL-0004774&mi=cdBiocompatible polymersPLGA nanoparticlesCellular uptakeCaco-2 cellsCytotoxicity |
| spellingShingle | J. H. Hamman L. A. Nkabinde L. N. N. Shoba-Zikhali B. Semete-Makokotlela L. Kalombo H. Swai A. Grobler Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity eXPRESS Polymer Letters Biocompatible polymers PLGA nanoparticles Cellular uptake Caco-2 cells Cytotoxicity |
| title | Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity |
| title_full | Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity |
| title_fullStr | Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity |
| title_full_unstemmed | Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity |
| title_short | Poly (D,L-lactide-co-glycolide) nanoparticles: Uptake by epithelial cells and cytotoxicity |
| title_sort | poly d l lactide co glycolide nanoparticles uptake by epithelial cells and cytotoxicity |
| topic | Biocompatible polymers PLGA nanoparticles Cellular uptake Caco-2 cells Cytotoxicity |
| url | http://www.expresspolymlett.com/letolt.php?file=EPL-0004774&mi=cd |
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