Optical widefield nuclear magnetic resonance microscopy
Abstract Microscopy enables detailed visualization and understanding of minute structures or processes. While cameras have significantly advanced optical, infrared, and electron microscopy, imaging nuclear magnetic resonance (NMR) signals on a camera has remained elusive. Here, we employ nitrogen-va...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55003-5 |
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| author | Karl D. Briegel Nick R. von Grafenstein Julia C. Draeger Peter Blümler Robin D. Allert Dominik B. Bucher |
| author_facet | Karl D. Briegel Nick R. von Grafenstein Julia C. Draeger Peter Blümler Robin D. Allert Dominik B. Bucher |
| author_sort | Karl D. Briegel |
| collection | DOAJ |
| description | Abstract Microscopy enables detailed visualization and understanding of minute structures or processes. While cameras have significantly advanced optical, infrared, and electron microscopy, imaging nuclear magnetic resonance (NMR) signals on a camera has remained elusive. Here, we employ nitrogen-vacancy centers in diamond as a quantum sensor, which converts NMR signals into optical signals that are subsequently captured by a high-speed camera. Unlike traditional magnetic resonance imaging, our method records the NMR signal over a wide field of view in real space. We demonstrate that our optical widefield NMR microscopy can image NMR signals in microfluidic structures with a ~10 μm resolution across a ~235 × 150 μm2 area. Crucially, each camera pixel records an NMR spectrum providing multicomponent information about the signal’s amplitude, phase, local magnetic field strengths, and gradients. The fusion of optical microscopy and NMR techniques enables multifaceted imaging applications in the physical and life sciences. |
| format | Article |
| id | doaj-art-916e4792b12142f1afb6ef9d49951fe0 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-916e4792b12142f1afb6ef9d49951fe02025-02-09T12:46:19ZengNature PortfolioNature Communications2041-17232025-02-011611810.1038/s41467-024-55003-5Optical widefield nuclear magnetic resonance microscopyKarl D. Briegel0Nick R. von Grafenstein1Julia C. Draeger2Peter Blümler3Robin D. Allert4Dominik B. Bucher5Technical University of Munich, TUM School of Natural Sciences, Department of ChemistryTechnical University of Munich, TUM School of Natural Sciences, Department of ChemistryTechnical University of Munich, TUM School of Natural Sciences, Department of ChemistryUniversity of Mainz, Institute of PhysicsTechnical University of Munich, TUM School of Natural Sciences, Department of ChemistryTechnical University of Munich, TUM School of Natural Sciences, Department of ChemistryAbstract Microscopy enables detailed visualization and understanding of minute structures or processes. While cameras have significantly advanced optical, infrared, and electron microscopy, imaging nuclear magnetic resonance (NMR) signals on a camera has remained elusive. Here, we employ nitrogen-vacancy centers in diamond as a quantum sensor, which converts NMR signals into optical signals that are subsequently captured by a high-speed camera. Unlike traditional magnetic resonance imaging, our method records the NMR signal over a wide field of view in real space. We demonstrate that our optical widefield NMR microscopy can image NMR signals in microfluidic structures with a ~10 μm resolution across a ~235 × 150 μm2 area. Crucially, each camera pixel records an NMR spectrum providing multicomponent information about the signal’s amplitude, phase, local magnetic field strengths, and gradients. The fusion of optical microscopy and NMR techniques enables multifaceted imaging applications in the physical and life sciences.https://doi.org/10.1038/s41467-024-55003-5 |
| spellingShingle | Karl D. Briegel Nick R. von Grafenstein Julia C. Draeger Peter Blümler Robin D. Allert Dominik B. Bucher Optical widefield nuclear magnetic resonance microscopy Nature Communications |
| title | Optical widefield nuclear magnetic resonance microscopy |
| title_full | Optical widefield nuclear magnetic resonance microscopy |
| title_fullStr | Optical widefield nuclear magnetic resonance microscopy |
| title_full_unstemmed | Optical widefield nuclear magnetic resonance microscopy |
| title_short | Optical widefield nuclear magnetic resonance microscopy |
| title_sort | optical widefield nuclear magnetic resonance microscopy |
| url | https://doi.org/10.1038/s41467-024-55003-5 |
| work_keys_str_mv | AT karldbriegel opticalwidefieldnuclearmagneticresonancemicroscopy AT nickrvongrafenstein opticalwidefieldnuclearmagneticresonancemicroscopy AT juliacdraeger opticalwidefieldnuclearmagneticresonancemicroscopy AT peterblumler opticalwidefieldnuclearmagneticresonancemicroscopy AT robindallert opticalwidefieldnuclearmagneticresonancemicroscopy AT dominikbbucher opticalwidefieldnuclearmagneticresonancemicroscopy |