A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides
A 13 weeks feeding assessment was performed with the aim of determining the influence of enzyme-treated soy protein (ETSP) supplementation on growth performance, intestinal barrier function, liver lipid metabolism and related signaling pathways of largemouth bass (Micropterus salmoides). A total of...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Journal of Agriculture and Food Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266615432500523X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849713308597223424 |
|---|---|
| author | Ju Zhao Quanquan Cao Min Jin Tingwen Shen Laia Ribas Haifeng Liu Jun Jiang |
| author_facet | Ju Zhao Quanquan Cao Min Jin Tingwen Shen Laia Ribas Haifeng Liu Jun Jiang |
| author_sort | Ju Zhao |
| collection | DOAJ |
| description | A 13 weeks feeding assessment was performed with the aim of determining the influence of enzyme-treated soy protein (ETSP) supplementation on growth performance, intestinal barrier function, liver lipid metabolism and related signaling pathways of largemouth bass (Micropterus salmoides). A total of 1000 largemouth bass (initial average weight: 22.3 ± 0.1 g) were fed five different diets used in the experiment, each with a different amount of ETSP: 0.0 (M1), 20.0 (M2), 40.0 (M3), 60.0 (M4), and 80.0 (M5) g/kg. The results demonstrated that dietary ETSP supplementation significantly improved growth performance, body composition, and nutrient retention (P<0.05). Histological examination revealed significantly greater intestinal villus height in fish fed optimal ETSP levels (P<0.05). The 40.0 g/kg ETSP diet enhanced intestinal barrier function by upregulating tight junction proteins and anti-inflammatory cytokines while downregulating pro-inflammatory factors through modulation of the AMPK-SIRT1-NF-κB signaling pathway (P<0.05). Moreover, histological examination revealed optimal ETSP (40.0 g/kg) improved hepatic macrovesicular steatosis and cellular nuclei displacement, restrain lipid vacuoles' relative area, also activities of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST), thereby lowered liver damage. Dietary ETSP may suppress fatty acid transport and lipid synthesis, and promote lipid catabolism by activating FGF21/AMPK pathway. Finally, quadratic regression analysis of the specific growth rate (SGR) revealed that the optimal dietary ETSP level for largemouth bass was 40.0 g/kg. |
| format | Article |
| id | doaj-art-91654a30f5f541b4bb47f5acdf35ed01 |
| institution | DOAJ |
| issn | 2666-1543 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Agriculture and Food Research |
| spelling | doaj-art-91654a30f5f541b4bb47f5acdf35ed012025-08-20T03:13:59ZengElsevierJournal of Agriculture and Food Research2666-15432025-08-012210215210.1016/j.jafr.2025.102152A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoidesJu Zhao0Quanquan Cao1Min Jin2Tingwen Shen3Laia Ribas4Haifeng Liu5Jun Jiang6College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, ChinaInstitut de Ciències del Mar, Consejo Superior de Investigaciones Científicas (ICM-CSIC), Barcelona, SpainCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China; Corresponding author.College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China; Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Ya’ an, 625014, China; Corresponding author. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.A 13 weeks feeding assessment was performed with the aim of determining the influence of enzyme-treated soy protein (ETSP) supplementation on growth performance, intestinal barrier function, liver lipid metabolism and related signaling pathways of largemouth bass (Micropterus salmoides). A total of 1000 largemouth bass (initial average weight: 22.3 ± 0.1 g) were fed five different diets used in the experiment, each with a different amount of ETSP: 0.0 (M1), 20.0 (M2), 40.0 (M3), 60.0 (M4), and 80.0 (M5) g/kg. The results demonstrated that dietary ETSP supplementation significantly improved growth performance, body composition, and nutrient retention (P<0.05). Histological examination revealed significantly greater intestinal villus height in fish fed optimal ETSP levels (P<0.05). The 40.0 g/kg ETSP diet enhanced intestinal barrier function by upregulating tight junction proteins and anti-inflammatory cytokines while downregulating pro-inflammatory factors through modulation of the AMPK-SIRT1-NF-κB signaling pathway (P<0.05). Moreover, histological examination revealed optimal ETSP (40.0 g/kg) improved hepatic macrovesicular steatosis and cellular nuclei displacement, restrain lipid vacuoles' relative area, also activities of plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST), thereby lowered liver damage. Dietary ETSP may suppress fatty acid transport and lipid synthesis, and promote lipid catabolism by activating FGF21/AMPK pathway. Finally, quadratic regression analysis of the specific growth rate (SGR) revealed that the optimal dietary ETSP level for largemouth bass was 40.0 g/kg.http://www.sciencedirect.com/science/article/pii/S266615432500523XEnzyme-treated soy proteinIntestinal barrier functionLipid metabolismInflammationTight junction |
| spellingShingle | Ju Zhao Quanquan Cao Min Jin Tingwen Shen Laia Ribas Haifeng Liu Jun Jiang A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides Journal of Agriculture and Food Research Enzyme-treated soy protein Intestinal barrier function Lipid metabolism Inflammation Tight junction |
| title | A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides |
| title_full | A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides |
| title_fullStr | A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides |
| title_full_unstemmed | A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides |
| title_short | A comprehensive study of enzyme-treated soy protein on intestinal barrier integrity, inflammatory responses, and liver lipid metabolism in Micropterus salmoides |
| title_sort | comprehensive study of enzyme treated soy protein on intestinal barrier integrity inflammatory responses and liver lipid metabolism in micropterus salmoides |
| topic | Enzyme-treated soy protein Intestinal barrier function Lipid metabolism Inflammation Tight junction |
| url | http://www.sciencedirect.com/science/article/pii/S266615432500523X |
| work_keys_str_mv | AT juzhao acomprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT quanquancao acomprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT minjin acomprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT tingwenshen acomprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT laiaribas acomprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT haifengliu acomprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT junjiang acomprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT juzhao comprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT quanquancao comprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT minjin comprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT tingwenshen comprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT laiaribas comprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT haifengliu comprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides AT junjiang comprehensivestudyofenzymetreatedsoyproteinonintestinalbarrierintegrityinflammatoryresponsesandliverlipidmetabolisminmicropterussalmoides |