The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soils
Agricultural soil microbiomes, with their varied cell sizes and metabolic capabilities, contribute significantly to differences in soil ecosystem functions and services. However, the relationships among bacterial cell size, community structure and nutrient turnover in agricultural soils remain uncle...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-04-01
|
| Series: | Geoderma |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0016706125000795 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850203572729282560 |
|---|---|
| author | Xuemei Hu Chang Wang Siyuan Wang Yinghui Cao Haofei Zhang Chang Liu He Sun Yajun Gao Shanshan Yang Sanfeng Chen Sen Du Gehong Wei Weimin Chen |
| author_facet | Xuemei Hu Chang Wang Siyuan Wang Yinghui Cao Haofei Zhang Chang Liu He Sun Yajun Gao Shanshan Yang Sanfeng Chen Sen Du Gehong Wei Weimin Chen |
| author_sort | Xuemei Hu |
| collection | DOAJ |
| description | Agricultural soil microbiomes, with their varied cell sizes and metabolic capabilities, contribute significantly to differences in soil ecosystem functions and services. However, the relationships among bacterial cell size, community structure and nutrient turnover in agricultural soils remain unclear. This study categorized bacterial cells from maize and soybean fields into five distinct size fractions—F1 (>10 μm), F2 (3–10 μm), F3 (1–3 μm), F4 (0.4–1 μm) and F5 (0.2–0.4 μm)—using polycarbonate membrane filtration. High-throughput sequencing of the 16S rRNA gene and soil incubation subsequently revealed the taxonomic composition and potential functions of each size fraction. The results indicated that the bacterial diversity in maize field soil was greater than that in soybean field soil, with the F4 size fraction exhibiting the highest diversity and abundance in both soils, whereas the F1 size fraction showed the lowest. Proteobacteria dominated across all size fractions, and size-specific taxonomic distributions were observed: Myxococcota, Entotheonellaeota and Cyanobacteria were enriched in F1 and F2; Planctomycetota and Chloroflexi were enriched in F3; Bacteroidota, Verrucomicrobiota, Actinobacteriota and Firmicutes were enriched in F4 and F5. Before incubation, the qPCR of functional genes showed that the F1–F3 fractions exhibited highly active ammonia oxidation (F1, F2) and ammonification (F3), while the F4 fraction presented highly efficient organic P mineralization, when compared to other fractions. After incubation, qPCR and soil property analyses revealed that the F4 fraction exhibited highest increase in cell numbers and regulated C and P turnover through the secretion of BG and AKP, whereas the F1 fraction consistently maintained high ammonia oxidation capacity. These findings illustrate that bacterial taxa vary in cell size and nutrient turnover processes under different land-uses, thereby deepening our understanding of the bacterial ecology in farmlands. |
| format | Article |
| id | doaj-art-9161d0459cde4880a84fda2e411c2cab |
| institution | OA Journals |
| issn | 1872-6259 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Geoderma |
| spelling | doaj-art-9161d0459cde4880a84fda2e411c2cab2025-08-20T02:11:29ZengElsevierGeoderma1872-62592025-04-0145611724110.1016/j.geoderma.2025.117241The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soilsXuemei Hu0Chang Wang1Siyuan Wang2Yinghui Cao3Haofei Zhang4Chang Liu5He Sun6Yajun Gao7Shanshan Yang8Sanfeng Chen9Sen Du10Gehong Wei11Weimin Chen12State Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Agrobiotechnology and College of Biological Sciences, China Agricultural University, Beijing 100193, People's Republic of ChinaFertilizer Technology Department, National Agricultural Extension Service Center, Beijing 100125, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of ChinaState Key Laboratory for Crop Stress Resistance and High-Efficiency Production, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, College of Life Sciences, Northwest A&F University, Yangling 712100 Shaanxi, People's Republic of China; Corresponding author.Agricultural soil microbiomes, with their varied cell sizes and metabolic capabilities, contribute significantly to differences in soil ecosystem functions and services. However, the relationships among bacterial cell size, community structure and nutrient turnover in agricultural soils remain unclear. This study categorized bacterial cells from maize and soybean fields into five distinct size fractions—F1 (>10 μm), F2 (3–10 μm), F3 (1–3 μm), F4 (0.4–1 μm) and F5 (0.2–0.4 μm)—using polycarbonate membrane filtration. High-throughput sequencing of the 16S rRNA gene and soil incubation subsequently revealed the taxonomic composition and potential functions of each size fraction. The results indicated that the bacterial diversity in maize field soil was greater than that in soybean field soil, with the F4 size fraction exhibiting the highest diversity and abundance in both soils, whereas the F1 size fraction showed the lowest. Proteobacteria dominated across all size fractions, and size-specific taxonomic distributions were observed: Myxococcota, Entotheonellaeota and Cyanobacteria were enriched in F1 and F2; Planctomycetota and Chloroflexi were enriched in F3; Bacteroidota, Verrucomicrobiota, Actinobacteriota and Firmicutes were enriched in F4 and F5. Before incubation, the qPCR of functional genes showed that the F1–F3 fractions exhibited highly active ammonia oxidation (F1, F2) and ammonification (F3), while the F4 fraction presented highly efficient organic P mineralization, when compared to other fractions. After incubation, qPCR and soil property analyses revealed that the F4 fraction exhibited highest increase in cell numbers and regulated C and P turnover through the secretion of BG and AKP, whereas the F1 fraction consistently maintained high ammonia oxidation capacity. These findings illustrate that bacterial taxa vary in cell size and nutrient turnover processes under different land-uses, thereby deepening our understanding of the bacterial ecology in farmlands.http://www.sciencedirect.com/science/article/pii/S0016706125000795Cell sizeCommunity structuresFarmland soilsSoil incubationNutrient turnover |
| spellingShingle | Xuemei Hu Chang Wang Siyuan Wang Yinghui Cao Haofei Zhang Chang Liu He Sun Yajun Gao Shanshan Yang Sanfeng Chen Sen Du Gehong Wei Weimin Chen The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soils Geoderma Cell size Community structures Farmland soils Soil incubation Nutrient turnover |
| title | The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soils |
| title_full | The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soils |
| title_fullStr | The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soils |
| title_full_unstemmed | The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soils |
| title_short | The diversity, composition and potential function of bacterial size fractions from maize and soybean farmland soils |
| title_sort | diversity composition and potential function of bacterial size fractions from maize and soybean farmland soils |
| topic | Cell size Community structures Farmland soils Soil incubation Nutrient turnover |
| url | http://www.sciencedirect.com/science/article/pii/S0016706125000795 |
| work_keys_str_mv | AT xuemeihu thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT changwang thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT siyuanwang thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT yinghuicao thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT haofeizhang thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT changliu thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT hesun thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT yajungao thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT shanshanyang thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT sanfengchen thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT sendu thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT gehongwei thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT weiminchen thediversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT xuemeihu diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT changwang diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT siyuanwang diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT yinghuicao diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT haofeizhang diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT changliu diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT hesun diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT yajungao diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT shanshanyang diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT sanfengchen diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT sendu diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT gehongwei diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils AT weiminchen diversitycompositionandpotentialfunctionofbacterialsizefractionsfrommaizeandsoybeanfarmlandsoils |