Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort
We compared different antineutrophil cytoplasmic antibody (ANCA) detection methods using a predominantly myeloperoxidase (MPO)-ANCA-associated vasculitis cohort. Stored sera from 147 patients with untreated ANCA-associated vasculitis (AAV), including microscopic polyangiitis and granulomatosis with...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-01-01
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| Series: | Immunological Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/25785826.2024.2408054 |
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| author | Yasuhiro Katsumata Ken-ei Sada Tomohiro Kameda Hiroaki Dobashi Shinya Kaname Naotake Tsuboi Yoshinori Matsumoto Koichi Amano Naoto Tamura Masayoshi Harigai |
| author_facet | Yasuhiro Katsumata Ken-ei Sada Tomohiro Kameda Hiroaki Dobashi Shinya Kaname Naotake Tsuboi Yoshinori Matsumoto Koichi Amano Naoto Tamura Masayoshi Harigai |
| author_sort | Yasuhiro Katsumata |
| collection | DOAJ |
| description | We compared different antineutrophil cytoplasmic antibody (ANCA) detection methods using a predominantly myeloperoxidase (MPO)-ANCA-associated vasculitis cohort. Stored sera from 147 patients with untreated ANCA-associated vasculitis (AAV), including microscopic polyangiitis and granulomatosis with polyangiitis (n = 115 and 32, respectively), and 124 disease controls were tested for P-ANCA and C-ANCA with immunofluorescence (IIF), and for MPO-ANCA and proteinase 3 (PR3)-ANCA with different antigen-specific immunoassays: direct enzyme-linked immunosorbent assay (ELISA), chemiluminescent enzyme immunoassay (CLEIA), third-generation fluorescent enzyme immunoassay (FEIA), and latex turbidimetrical immunoassay (LTIA). In addition, MPO-ANCA and PR3-ANCA titers were calibrated using certified reference materials (CRMs). The sensitivities and specificities for AAV diagnoses were 95% and 94% (IIF), 86% and 98% (ELISA), 93% and 94% (CLEIA), 92% and 96% (FEIA), and 68% and 88% (LTIA). Dual IIF/antigen-specific immunoassay testing reduced diagnostic accuracies from 94% to 93%. The quantitative agreement between ANCA levels measured using CLEIA and FEIA and calibrated using CRMs was not good. In conclusion, this study demonstrated the high performance of antigen-specific immunoassays for AAV diagnosis in a predominantly MPO-ANCA-associated vasculitis cohort and suggested that the benefit of dual IIF/antigen-specific immunoassay testing is limited. Standardizing ANCA measurements using different immunoassays was difficult, even when using CRMs. |
| format | Article |
| id | doaj-art-91541eea9fec47289935e5fbc7af9da4 |
| institution | DOAJ |
| issn | 2578-5826 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Immunological Medicine |
| spelling | doaj-art-91541eea9fec47289935e5fbc7af9da42025-08-20T03:12:51ZengTaylor & Francis GroupImmunological Medicine2578-58262025-01-01481475710.1080/25785826.2024.2408054Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohortYasuhiro Katsumata0Ken-ei Sada1Tomohiro Kameda2Hiroaki Dobashi3Shinya Kaname4Naotake Tsuboi5Yoshinori Matsumoto6Koichi Amano7Naoto Tamura8Masayoshi Harigai9Division of Rheumatology, Department of Internal Medicine, Tokyo Women’s Medical University School of Medicine, Tokyo, JapanDepartment of Clinical Epidemiology, Kochi Medical School, Nankoku, JapanDepartment of Rheumatology, Takamatsu Red Cross Hospital, Kagawa, JapanDivision of Hematology, Rheumatology and Respiratory Medicine, Department of Internal Medicine, Faculty of Medicine, Kagawa University, Kagawa, JapanDepartment of Nephrology and Rheumatology, Kyorin University School of Medicine, Tokyo, JapanDepartment of Nephrology, Fujita Health University School of Medicine, Toyoake, JapanDepartment of Nephrology, Rheumatology, Endocrinology and Metabolism, Okayama University Faculty of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, JapanDepartment of Rheumatology and Clinical Immunology, Saitama Medical Center, Saitama Medical University, Kawagoe, JapanDepartment of Internal Medicine and Rheumatology, Juntendo University School of Medicine, Tokyo, JapanDivision of Rheumatology, Department of Internal Medicine, Tokyo Women’s Medical University School of Medicine, Tokyo, JapanWe compared different antineutrophil cytoplasmic antibody (ANCA) detection methods using a predominantly myeloperoxidase (MPO)-ANCA-associated vasculitis cohort. Stored sera from 147 patients with untreated ANCA-associated vasculitis (AAV), including microscopic polyangiitis and granulomatosis with polyangiitis (n = 115 and 32, respectively), and 124 disease controls were tested for P-ANCA and C-ANCA with immunofluorescence (IIF), and for MPO-ANCA and proteinase 3 (PR3)-ANCA with different antigen-specific immunoassays: direct enzyme-linked immunosorbent assay (ELISA), chemiluminescent enzyme immunoassay (CLEIA), third-generation fluorescent enzyme immunoassay (FEIA), and latex turbidimetrical immunoassay (LTIA). In addition, MPO-ANCA and PR3-ANCA titers were calibrated using certified reference materials (CRMs). The sensitivities and specificities for AAV diagnoses were 95% and 94% (IIF), 86% and 98% (ELISA), 93% and 94% (CLEIA), 92% and 96% (FEIA), and 68% and 88% (LTIA). Dual IIF/antigen-specific immunoassay testing reduced diagnostic accuracies from 94% to 93%. The quantitative agreement between ANCA levels measured using CLEIA and FEIA and calibrated using CRMs was not good. In conclusion, this study demonstrated the high performance of antigen-specific immunoassays for AAV diagnosis in a predominantly MPO-ANCA-associated vasculitis cohort and suggested that the benefit of dual IIF/antigen-specific immunoassay testing is limited. Standardizing ANCA measurements using different immunoassays was difficult, even when using CRMs.https://www.tandfonline.com/doi/10.1080/25785826.2024.2408054Antineutrophil cytoplasm antibodymicroscopic polyangiitisgranulomatosis with polyangiitislaboratory diagnosis |
| spellingShingle | Yasuhiro Katsumata Ken-ei Sada Tomohiro Kameda Hiroaki Dobashi Shinya Kaname Naotake Tsuboi Yoshinori Matsumoto Koichi Amano Naoto Tamura Masayoshi Harigai Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort Immunological Medicine Antineutrophil cytoplasm antibody microscopic polyangiitis granulomatosis with polyangiitis laboratory diagnosis |
| title | Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort |
| title_full | Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort |
| title_fullStr | Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort |
| title_full_unstemmed | Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort |
| title_short | Comparison of different ANCA detection methods in a predominantly MPO-ANCA-associated vasculitis cohort |
| title_sort | comparison of different anca detection methods in a predominantly mpo anca associated vasculitis cohort |
| topic | Antineutrophil cytoplasm antibody microscopic polyangiitis granulomatosis with polyangiitis laboratory diagnosis |
| url | https://www.tandfonline.com/doi/10.1080/25785826.2024.2408054 |
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