Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in Water

In recent years, Thailand’s agriculture sector has seen a significant rise in pesticide usage due to its vital role in the economy and global food trade. However, the uncontrolled use of pesticides poses severe health and environmental risks. This research focuses on the detection of pesticide resid...

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Main Authors: Chiravoot Pechyen, Calin Trif, Benya Cherdhirunkorn, Surachet Toommee, Yardnapar Parcharoen
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Analytica
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Online Access:https://www.mdpi.com/2673-4532/6/1/4
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author Chiravoot Pechyen
Calin Trif
Benya Cherdhirunkorn
Surachet Toommee
Yardnapar Parcharoen
author_facet Chiravoot Pechyen
Calin Trif
Benya Cherdhirunkorn
Surachet Toommee
Yardnapar Parcharoen
author_sort Chiravoot Pechyen
collection DOAJ
description In recent years, Thailand’s agriculture sector has seen a significant rise in pesticide usage due to its vital role in the economy and global food trade. However, the uncontrolled use of pesticides poses severe health and environmental risks. This research focuses on the detection of pesticide residues, particularly carbaryl and paraoxon-ethyl, which are prevalent due to their high efficacy in pest control but pose neurological health risks by inhibiting acetylcholinesterase (AChE) activity, potentially impacting human health. The developed method assesses pesticide concentration by measuring changes in the CIE-LAB color space using a smartphone camera. Testing several concentrations (0.2, 0.6, and 1.0 ppm) over time revealed significant differences via ANOVA (<i>F</i> = 32.8, <i>p</i> < 0.001) and a strong linear relationship with <i>R</i><sup>2</sup> values from 0.9129 to 0.9973 through regression analysis. Additionally, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were utilized to further understand the relationship between color changes and pesticide type and concentration. PCA showed that the L* and b* values contributed most to explaining the variance in the data (96.24%), while PLS-DA provided classification models. These strong linear relationships between pesticide concentration and colorimetric changes showcase the method’s accuracy and potential for on-site pesticide monitoring in agricultural produce, emphasizing technological advancements in sustainable agriculture practices. This research presents encouraging findings from an inexpensive and straightforward method for detecting pesticide residues, suggesting that a specialized mobile application could enhance the implementation of the proposed system.
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id doaj-art-915407a1db254b5caafdc4006ff7a74f
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issn 2673-4532
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publishDate 2025-02-01
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spelling doaj-art-915407a1db254b5caafdc4006ff7a74f2025-08-20T02:11:12ZengMDPI AGAnalytica2673-45322025-02-0161410.3390/analytica6010004Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in WaterChiravoot Pechyen0Calin Trif1Benya Cherdhirunkorn2Surachet Toommee3Yardnapar Parcharoen4Department of Materials and Textile Technology, Faculty of Science and Technology, Thammasat University, Pathum Thani 12120, ThailandChulabhorn International College of Medicine, Thammasat University, Pathum Thani 12120, ThailandCenter of Excellence in Modern Technology and Advanced Manufacturing for Medical Innovation, Thammasat University, Pathum Thani 12120, ThailandFaculty of Industrial Technology, Kamphaeng Phet Rajabhat University, Kamphaeng Phet 62000, ThailandChulabhorn International College of Medicine, Thammasat University, Pathum Thani 12120, ThailandIn recent years, Thailand’s agriculture sector has seen a significant rise in pesticide usage due to its vital role in the economy and global food trade. However, the uncontrolled use of pesticides poses severe health and environmental risks. This research focuses on the detection of pesticide residues, particularly carbaryl and paraoxon-ethyl, which are prevalent due to their high efficacy in pest control but pose neurological health risks by inhibiting acetylcholinesterase (AChE) activity, potentially impacting human health. The developed method assesses pesticide concentration by measuring changes in the CIE-LAB color space using a smartphone camera. Testing several concentrations (0.2, 0.6, and 1.0 ppm) over time revealed significant differences via ANOVA (<i>F</i> = 32.8, <i>p</i> < 0.001) and a strong linear relationship with <i>R</i><sup>2</sup> values from 0.9129 to 0.9973 through regression analysis. Additionally, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were utilized to further understand the relationship between color changes and pesticide type and concentration. PCA showed that the L* and b* values contributed most to explaining the variance in the data (96.24%), while PLS-DA provided classification models. These strong linear relationships between pesticide concentration and colorimetric changes showcase the method’s accuracy and potential for on-site pesticide monitoring in agricultural produce, emphasizing technological advancements in sustainable agriculture practices. This research presents encouraging findings from an inexpensive and straightforward method for detecting pesticide residues, suggesting that a specialized mobile application could enhance the implementation of the proposed system.https://www.mdpi.com/2673-4532/6/1/4acetylcholinesterase (ACHE)paraoxon ethylcarbarylsustainable development goal 3 (SDG 3)water contaminationfood safety
spellingShingle Chiravoot Pechyen
Calin Trif
Benya Cherdhirunkorn
Surachet Toommee
Yardnapar Parcharoen
Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in Water
Analytica
acetylcholinesterase (ACHE)
paraoxon ethyl
carbaryl
sustainable development goal 3 (SDG 3)
water contamination
food safety
title Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in Water
title_full Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in Water
title_fullStr Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in Water
title_full_unstemmed Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in Water
title_short Measurement of Ellman’s Essay Using a Smartphone Camera Coupled with an Image Processing Technique in CIE-LAB Color Space for the Detection of Two Pesticides in Water
title_sort measurement of ellman s essay using a smartphone camera coupled with an image processing technique in cie lab color space for the detection of two pesticides in water
topic acetylcholinesterase (ACHE)
paraoxon ethyl
carbaryl
sustainable development goal 3 (SDG 3)
water contamination
food safety
url https://www.mdpi.com/2673-4532/6/1/4
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