Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro Study

Placing an intracanal medicament helps in the disinfection of primary root canals, and nanoparticles enhance the material properties by increasing the physical and chemical reactivity. The study was aimed at assessing the efficacy of calcium hydroxide+calcium hydroxide nanoparticles and zinc oxide+z...

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Main Authors: Azra Kaukab, Sridhar Nekkanti
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/tswj/5182716
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author Azra Kaukab
Sridhar Nekkanti
author_facet Azra Kaukab
Sridhar Nekkanti
author_sort Azra Kaukab
collection DOAJ
description Placing an intracanal medicament helps in the disinfection of primary root canals, and nanoparticles enhance the material properties by increasing the physical and chemical reactivity. The study was aimed at assessing the efficacy of calcium hydroxide+calcium hydroxide nanoparticles and zinc oxide+zinc oxide nanoparticles as an intracanal medicament when compared with calcium hydroxide and zinc oxide eugenol in terms of antibacterial efficacy and penetration depth. Forty extracted human primary teeth were included in the study. Of these, 40 teeth were divided into two subgroups—Group A (to assess antibacterial efficacy) and Group B (to assess penetration depth). The teeth in Group A were contaminated with an Enterococcus faecalis biofilm for 7 days, and CFU count was determined. Calcium hydroxide (A1), calcium hydroxide with calcium hydroxide nanoparticles (A2), zinc oxide with zinc oxide nanoparticles (A3), and zinc oxide eugenol (A4) were placed in the canals. CFU count was determined on Day 7 and Day 14. The teeth in Group B were also divided into calcium hydroxide (B1), calcium hydroxide with calcium hydroxide nanoparticles (B2), zinc oxide with zinc oxide nanoparticles (B3), and zinc oxide eugenol (B4) groups. Two teeth from each group were subjected to SEM analysis. A statistically significant difference was noted in the CFU count reduction on Day 7 in Group A2, Group A3, and Group A4. The highest penetration depth was noted in Group B2. The authors concluded that the antibacterial efficacy and penetration depth into dentin improved on incorporating calcium hydroxide and zinc oxide nanoparticles. Based on this conclusion, the authors recommend further in vivo studies with nanoparticles incorporated intracanal medicaments in primary teeth to determine their safety of use and feasibility in a clinical scenario.
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spelling doaj-art-9124716a95644daeb7d7c3ae49427ced2025-08-20T03:31:24ZengWileyThe Scientific World Journal1537-744X2025-01-01202510.1155/tswj/5182716Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro StudyAzra Kaukab0Sridhar Nekkanti1Department of Pediatric and Preventive DentistryDepartment of Pediatric and Preventive DentistryPlacing an intracanal medicament helps in the disinfection of primary root canals, and nanoparticles enhance the material properties by increasing the physical and chemical reactivity. The study was aimed at assessing the efficacy of calcium hydroxide+calcium hydroxide nanoparticles and zinc oxide+zinc oxide nanoparticles as an intracanal medicament when compared with calcium hydroxide and zinc oxide eugenol in terms of antibacterial efficacy and penetration depth. Forty extracted human primary teeth were included in the study. Of these, 40 teeth were divided into two subgroups—Group A (to assess antibacterial efficacy) and Group B (to assess penetration depth). The teeth in Group A were contaminated with an Enterococcus faecalis biofilm for 7 days, and CFU count was determined. Calcium hydroxide (A1), calcium hydroxide with calcium hydroxide nanoparticles (A2), zinc oxide with zinc oxide nanoparticles (A3), and zinc oxide eugenol (A4) were placed in the canals. CFU count was determined on Day 7 and Day 14. The teeth in Group B were also divided into calcium hydroxide (B1), calcium hydroxide with calcium hydroxide nanoparticles (B2), zinc oxide with zinc oxide nanoparticles (B3), and zinc oxide eugenol (B4) groups. Two teeth from each group were subjected to SEM analysis. A statistically significant difference was noted in the CFU count reduction on Day 7 in Group A2, Group A3, and Group A4. The highest penetration depth was noted in Group B2. The authors concluded that the antibacterial efficacy and penetration depth into dentin improved on incorporating calcium hydroxide and zinc oxide nanoparticles. Based on this conclusion, the authors recommend further in vivo studies with nanoparticles incorporated intracanal medicaments in primary teeth to determine their safety of use and feasibility in a clinical scenario.http://dx.doi.org/10.1155/tswj/5182716
spellingShingle Azra Kaukab
Sridhar Nekkanti
Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro Study
The Scientific World Journal
title Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro Study
title_full Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro Study
title_fullStr Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro Study
title_full_unstemmed Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro Study
title_short Antimicrobial Efficacy of Intracanal Medicaments Incorporated With Nanoparticles in Primary Teeth: An In Vitro Study
title_sort antimicrobial efficacy of intracanal medicaments incorporated with nanoparticles in primary teeth an in vitro study
url http://dx.doi.org/10.1155/tswj/5182716
work_keys_str_mv AT azrakaukab antimicrobialefficacyofintracanalmedicamentsincorporatedwithnanoparticlesinprimaryteethaninvitrostudy
AT sridharnekkanti antimicrobialefficacyofintracanalmedicamentsincorporatedwithnanoparticlesinprimaryteethaninvitrostudy