Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating
Multifocus microscopy has previously been demonstrated to provide volumetric information from a single shot. However, the practical application of this method is challenging due to its weak optical sectioning and limited spatial resolution. Here, we report on the combination of a distorted diffracti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/11/550 |
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| author | Siwei Li Yunke Zhang Zhiwen Liao Zengyuan Tian Hairulazwan Hashim Youjun Zeng Yandong Zhang |
| author_facet | Siwei Li Yunke Zhang Zhiwen Liao Zengyuan Tian Hairulazwan Hashim Youjun Zeng Yandong Zhang |
| author_sort | Siwei Li |
| collection | DOAJ |
| description | Multifocus microscopy has previously been demonstrated to provide volumetric information from a single shot. However, the practical application of this method is challenging due to its weak optical sectioning and limited spatial resolution. Here, we report on the combination of a distorted diffraction grating and multifocal scanning illumination microscopy to improve spatial resolution and contrast. DG is introduced in the emission path of the multifocal scanning illumination microscopy, which splits the fluorescence signal from different sample layers into different diffraction orders. After postprocessing, super-resolution wide-field images of different sample layers can be reconstructed from single 2D scanning. |
| format | Article |
| id | doaj-art-9124485c84bf464fb3674080fcce81f4 |
| institution | OA Journals |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-9124485c84bf464fb3674080fcce81f42025-08-20T01:53:44ZengMDPI AGBiosensors2079-63742024-11-01141155010.3390/bios14110550Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction GratingSiwei Li0Yunke Zhang1Zhiwen Liao2Zengyuan Tian3Hairulazwan Hashim4Youjun Zeng5Yandong Zhang6College of Mechanical Electronical and Engineering, Zhuhai City Polytechnic, Zhuhai 519000, ChinaCollege of Mechanical Electronical and Engineering, Zhuhai City Polytechnic, Zhuhai 519000, ChinaCollege of Mechanical Electronical and Engineering, Zhuhai City Polytechnic, Zhuhai 519000, ChinaCollege of Mechanical Electronical and Engineering, Zhuhai City Polytechnic, Zhuhai 519000, ChinaDepartment of Electrical Engineering Technology, Universiti Tun Hussein Onn Malaysia, Panchor 84600, MalaysiaSchool of Physics & Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, ChinaCollege of Mechanical Electronical and Engineering, Zhuhai City Polytechnic, Zhuhai 519000, ChinaMultifocus microscopy has previously been demonstrated to provide volumetric information from a single shot. However, the practical application of this method is challenging due to its weak optical sectioning and limited spatial resolution. Here, we report on the combination of a distorted diffraction grating and multifocal scanning illumination microscopy to improve spatial resolution and contrast. DG is introduced in the emission path of the multifocal scanning illumination microscopy, which splits the fluorescence signal from different sample layers into different diffraction orders. After postprocessing, super-resolution wide-field images of different sample layers can be reconstructed from single 2D scanning.https://www.mdpi.com/2079-6374/14/11/550super resolutionmicroscopybiomedical photonics |
| spellingShingle | Siwei Li Yunke Zhang Zhiwen Liao Zengyuan Tian Hairulazwan Hashim Youjun Zeng Yandong Zhang Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating Biosensors super resolution microscopy biomedical photonics |
| title | Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating |
| title_full | Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating |
| title_fullStr | Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating |
| title_full_unstemmed | Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating |
| title_short | Bi-Plane Multicolor Scanning Illumination Microscopy with Multispot Excitation and a Distorted Diffraction Grating |
| title_sort | bi plane multicolor scanning illumination microscopy with multispot excitation and a distorted diffraction grating |
| topic | super resolution microscopy biomedical photonics |
| url | https://www.mdpi.com/2079-6374/14/11/550 |
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