Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function
This study found that in patients with SLE (n = 5), lethal (let)-7f-5p expression was significantly downregulated in peripheral blood mononuclear cells. Further, high-throughput RNA sequencing was used to mine the differential transcriptome expression in renal tissue exosomes of systemic lupus eryth...
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| Format: | Article |
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Non-coding RNA Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468054025000265 |
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| author | Yi-jing Liu Hai-bing Miao Shu Lin Zhen Chen |
| author_facet | Yi-jing Liu Hai-bing Miao Shu Lin Zhen Chen |
| author_sort | Yi-jing Liu |
| collection | DOAJ |
| description | This study found that in patients with SLE (n = 5), lethal (let)-7f-5p expression was significantly downregulated in peripheral blood mononuclear cells. Further, high-throughput RNA sequencing was used to mine the differential transcriptome expression in renal tissue exosomes of systemic lupus erythematosus (SLE)-prone mice, and bioinformatics was utilized to analyze non-coding RNAs and coding RNAs in exosomes for their possible roles in SLE. In renal tissues of MRL/lpr SLE-prone mice with exosomes and Pristane-induced SLE mice, we also demonstrated aberrant expression levels of microRNA (miRNA) let-7f-5p. Meanwhile, in the macrophage inflammation model, the expression levels of let-7f-5p were downregulated, that of guanylate binding protein (Gbp2 and Gbp7) were upregulated, and the inflammatory state of macrophages was alleviated following transfection with the let-7f-5p mimic. Co-culturing mesenchymal stem cells with a macrophage model of inflammation resulted in increased let-7f-5p expression and downregulated inflammatory factors, Gbp2 and Gbp7 expression in macrophages. Dual luciferase reporter gene assays confirmed that let-7f-5p directly binds to the 3′ UTR of Gbp7 to regulate its expression. Let-7f-5p regulation of the Gbp family is involved in SLE pathogenesis and is a biomarker associated with the inflammatory response with potential clinical applications. |
| format | Article |
| id | doaj-art-9121129d7b85416fa2ca60191ead57d7 |
| institution | OA Journals |
| issn | 2468-0540 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Non-coding RNA Research |
| spelling | doaj-art-9121129d7b85416fa2ca60191ead57d72025-08-20T02:38:30ZengKeAi Communications Co., Ltd.Non-coding RNA Research2468-05402025-06-011211613110.1016/j.ncrna.2025.02.004Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory functionYi-jing Liu0Hai-bing Miao1Shu Lin2Zhen Chen3Department of Rheumatology and Immunology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, ChinaDepartment of Rheumatology and Immunology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, ChinaCentre of Neurological and Metabolic Research, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China; Group of Neuroendocrinology, Garvan Institute of Medical Research, Sydney, Australia; Corresponding author. 34 Zhongshan Road, Quanzhou, 362000, China.Department of Rheumatology and Immunology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China; Corresponding author. 34 Zhongshan Road, Quanzhou, 362000, China.This study found that in patients with SLE (n = 5), lethal (let)-7f-5p expression was significantly downregulated in peripheral blood mononuclear cells. Further, high-throughput RNA sequencing was used to mine the differential transcriptome expression in renal tissue exosomes of systemic lupus erythematosus (SLE)-prone mice, and bioinformatics was utilized to analyze non-coding RNAs and coding RNAs in exosomes for their possible roles in SLE. In renal tissues of MRL/lpr SLE-prone mice with exosomes and Pristane-induced SLE mice, we also demonstrated aberrant expression levels of microRNA (miRNA) let-7f-5p. Meanwhile, in the macrophage inflammation model, the expression levels of let-7f-5p were downregulated, that of guanylate binding protein (Gbp2 and Gbp7) were upregulated, and the inflammatory state of macrophages was alleviated following transfection with the let-7f-5p mimic. Co-culturing mesenchymal stem cells with a macrophage model of inflammation resulted in increased let-7f-5p expression and downregulated inflammatory factors, Gbp2 and Gbp7 expression in macrophages. Dual luciferase reporter gene assays confirmed that let-7f-5p directly binds to the 3′ UTR of Gbp7 to regulate its expression. Let-7f-5p regulation of the Gbp family is involved in SLE pathogenesis and is a biomarker associated with the inflammatory response with potential clinical applications.http://www.sciencedirect.com/science/article/pii/S2468054025000265Systemic lupus erythematosusExosomesNon-coding RNA |
| spellingShingle | Yi-jing Liu Hai-bing Miao Shu Lin Zhen Chen Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function Non-coding RNA Research Systemic lupus erythematosus Exosomes Non-coding RNA |
| title | Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function |
| title_full | Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function |
| title_fullStr | Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function |
| title_full_unstemmed | Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function |
| title_short | Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function |
| title_sort | exosomes derived let 7f 5p is a potential biomarker of sle with anti inflammatory function |
| topic | Systemic lupus erythematosus Exosomes Non-coding RNA |
| url | http://www.sciencedirect.com/science/article/pii/S2468054025000265 |
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