Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst
In this study, evaporation-induced self-assembly was applied to prepare amine-functionalized nano-silica (NH2-Pr-SBA-15). That was simply used to immobilize Bacillus stratosphericus PSP8 lipase (E–NH2–Pr-SBA-15), producing a nanobiocatalyst with good stability under vigorous shaking and a maximum li...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | International Journal of Chemical Engineering |
| Online Access: | http://dx.doi.org/10.1155/2022/7899996 |
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| author | Abdallah R. Ismail Hamdy Kashtoh Mohamed A. Betiha Salem A. Abu Amr Kwang-Hyun Baek Nour Sh. El-Gendy |
| author_facet | Abdallah R. Ismail Hamdy Kashtoh Mohamed A. Betiha Salem A. Abu Amr Kwang-Hyun Baek Nour Sh. El-Gendy |
| author_sort | Abdallah R. Ismail |
| collection | DOAJ |
| description | In this study, evaporation-induced self-assembly was applied to prepare amine-functionalized nano-silica (NH2-Pr-SBA-15). That was simply used to immobilize Bacillus stratosphericus PSP8 lipase (E–NH2–Pr-SBA-15), producing a nanobiocatalyst with good stability under vigorous shaking and a maximum lipase activity of 45 ± 2 U/mL. High-resolution X-ray diffractometer, Fourier transform infrared spectroscopy, N2 adsorption-desorption, field-emission scanning electron, and high-resolution transmission electron microscopic analyses proved the successful SBA-15 functionalization and enzyme immobilization. Response surface methodology based on a 1/2 fraction-three-levels face center composite design was applied to optimize the biodiesel transesterification process. This expressed efficient percentage conversion (97.85%) and biodiesel yield (97.01%) under relatively mild operating conditions: 3.12 : 1 methanol to oil ratio, 3.08 wt.% E–NH2–Pr-SBA-15 loading, 48.6°C, 3.19 h at a mixing rate of 495.53 rpm. E–NH2–Pr-SBA-15 proved to have a long lifetime, operational stability, and reusability. |
| format | Article |
| id | doaj-art-9117e6d0bdb046c886f6464637d81a3c |
| institution | Kabale University |
| issn | 1687-8078 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Chemical Engineering |
| spelling | doaj-art-9117e6d0bdb046c886f6464637d81a3c2025-02-03T06:08:44ZengWileyInternational Journal of Chemical Engineering1687-80782022-01-01202210.1155/2022/7899996Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 NanobiocatalystAbdallah R. Ismail0Hamdy Kashtoh1Mohamed A. Betiha2Salem A. Abu Amr3Kwang-Hyun Baek4Nour Sh. El-Gendy5Department of BiotechnologyDepartment of BiotechnologyEgyptian Petroleum Research Institute (EPRI)Health Safety and Environmental ManagementDepartment of BiotechnologyEgyptian Petroleum Research Institute (EPRI)In this study, evaporation-induced self-assembly was applied to prepare amine-functionalized nano-silica (NH2-Pr-SBA-15). That was simply used to immobilize Bacillus stratosphericus PSP8 lipase (E–NH2–Pr-SBA-15), producing a nanobiocatalyst with good stability under vigorous shaking and a maximum lipase activity of 45 ± 2 U/mL. High-resolution X-ray diffractometer, Fourier transform infrared spectroscopy, N2 adsorption-desorption, field-emission scanning electron, and high-resolution transmission electron microscopic analyses proved the successful SBA-15 functionalization and enzyme immobilization. Response surface methodology based on a 1/2 fraction-three-levels face center composite design was applied to optimize the biodiesel transesterification process. This expressed efficient percentage conversion (97.85%) and biodiesel yield (97.01%) under relatively mild operating conditions: 3.12 : 1 methanol to oil ratio, 3.08 wt.% E–NH2–Pr-SBA-15 loading, 48.6°C, 3.19 h at a mixing rate of 495.53 rpm. E–NH2–Pr-SBA-15 proved to have a long lifetime, operational stability, and reusability.http://dx.doi.org/10.1155/2022/7899996 |
| spellingShingle | Abdallah R. Ismail Hamdy Kashtoh Mohamed A. Betiha Salem A. Abu Amr Kwang-Hyun Baek Nour Sh. El-Gendy Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst International Journal of Chemical Engineering |
| title | Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst |
| title_full | Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst |
| title_fullStr | Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst |
| title_full_unstemmed | Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst |
| title_short | Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst |
| title_sort | valorization of waste cooking oil into biodiesel via bacillus stratosphericus lipase amine functionalized mesoporous sba 15 nanobiocatalyst |
| url | http://dx.doi.org/10.1155/2022/7899996 |
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