Gas chromatography and mass spectrometry (GC-MS) analysis of supercritical CO<sub>2</sub> extract of flower bud of Chrysanthemum indicum and its antibacterial activity
Jumi, the flower bud of Chrysanthemum indicum L., is a special tea from Shilian, Zhejiang Province, and it is famous for its unique fragrance. Jumi essential oil has numerous efficacies and applications in the field of pharmacy and cosmetic industry, therefore developing effective oil extracting met...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2013-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2012.11.068 |
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| Summary: | Jumi, the flower bud of Chrysanthemum indicum L., is a special tea from Shilian, Zhejiang Province, and it is famous for its unique fragrance. Jumi essential oil has numerous efficacies and applications in the field of pharmacy and cosmetic industry, therefore developing effective oil extracting method which can keep both biological activity and fragrance is of considerable significance. At present, steam distillation has been reported as essential oil extract method, but this method can not keep the unique fragrance of Jumi due to the decomposition of fragrant component at high temperature. However, the supercritical fluid of CO 2 (SFE-CO<sub>2</sub>) is a low temperature processing technique and can fully keep the unique fragrance of Jumi, which is an efficient, nontoxic, pollution-free, and non-residual method to extract and separate Jumi essential oil.The objective of this paper is to evaluate the quality of Jumi essential oil extracted by SFE-CO<sub>2</sub> and determine its biological activity. The chemical compositions of Jumi essential oil were determined by GC-MS and their inhibitory activities against Staphylococcus aureus and Escherichia coli were analyzed by filter paper tablet bacteriostatic method and minimum inhibitory concentration (MIC).The results showed that the 28 types of components with the similarity of more than 70% were identified in Jumi essential oil by GC-MS analysis, which were mainly terpenes and their derivatives, and the former mainly include monoterpene and sesquiterpene, and the latter was mainly alcohols, for example, the contents of hexamethyl-benzene, 2- (2, 4-hexadiynylidene) -1, 6-dioxaspiro[4, 4] nonane-3-ene and (2 R-Cis) -1, 2, 3, 4, 4a, 5, 6, 7-octahydro-. alpha.,. alpha., 4a, 8-tetramethyl-2-naphthalenemethanol were higher than 9%, respectively. Bacterial inhibition tests indicated that Jumi essential oil could inhibit the growth of S. aureus and E. coli, and its MIC was 1.6 mg/mL.It is indicated that the Jumi essential oil extracted by SFE-CO<sub>2</sub> is non-toxic and pollution-free and it offers a scientific evidence about developing Jumi essential oil with high additional value and good-quality in cosmetic and food additive. |
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| ISSN: | 1008-9209 2097-5155 |