Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidics
The success of cell-based therapies strongly depends on the regenerative capacity of patient-derived cells, which can vary widely. Enhancing cell potency is therefore critical, especially for autologous applications. Biophysical treatment e.g. extracorporeal shockwave therapy (ESWT) has emerged as a...
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Elsevier
2025-06-01
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| Series: | Applications in Engineering Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666496825000147 |
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| author | Marlene Wahlmueller Bianca Buchegger Cyrill Slezak Heinz Redl Susanne Wolbank Eleni Priglinger Armin Hochreiner |
| author_facet | Marlene Wahlmueller Bianca Buchegger Cyrill Slezak Heinz Redl Susanne Wolbank Eleni Priglinger Armin Hochreiner |
| author_sort | Marlene Wahlmueller |
| collection | DOAJ |
| description | The success of cell-based therapies strongly depends on the regenerative capacity of patient-derived cells, which can vary widely. Enhancing cell potency is therefore critical, especially for autologous applications. Biophysical treatment e.g. extracorporeal shockwave therapy (ESWT) has emerged as a promising tool to enhance the regenerative potential of cells and has been applied in clinical practice for the treatment of several diseases. We developed a novel, low-cost, small and adaptable multi-mode pulse generating system (PGS) that enables direct treatment of cells in 3D-printed microfluidic devices. Adipose-derived cell treatment by our novel PGS showed first promising results, including significantly increased cellular adenosine triphosphate (ATP) release and proliferation. Enhanced cell functionality could be observed through a significantly increased adipogenic differentiation potential and a trend towards osteogenic and chondrogenic lineages. This novel approach offers unique characteristics achieved by its small dimensions and light weight that come along with increased flexibility and high integrability in existing systems and could therefore overcome limitations faced by conventional biophysical methods. It enables the combination of the process of cell treatment and live monitoring of cells and could therefore emerge in the field of bioprinting, in lab-on-a-chip applications as well as future clinical applications in cell-based therapies for many different therapeutic fields. |
| format | Article |
| id | doaj-art-91133d3b845443b8bfbe4e09a1ffc9ec |
| institution | DOAJ |
| issn | 2666-4968 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applications in Engineering Science |
| spelling | doaj-art-91133d3b845443b8bfbe4e09a1ffc9ec2025-08-20T03:19:57ZengElsevierApplications in Engineering Science2666-49682025-06-012210021610.1016/j.apples.2025.100216Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidicsMarlene Wahlmueller0Bianca Buchegger1Cyrill Slezak2Heinz Redl3Susanne Wolbank4Eleni Priglinger5Armin Hochreiner6Ludwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Linz/Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, AustriaSchool of Medical Engineering and Applied Social Science, University of Applied Sciences Upper Austria, Linz, Austria; Institute of Applied Physics, Johannes Kepler University, Linz, AustriaAustrian Cluster for Tissue Regeneration, Vienna, Austria; Department of Physics, Utah Valley University, Orem, UT 84059, USALudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Linz/Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Linz/Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, AustriaLudwig Boltzmann Institute for Traumatology, The Research Center in Cooperation with AUVA, Linz/Vienna, Austria; Austrian Cluster for Tissue Regeneration, Vienna, Austria; Department for Orthopaedics and Traumatology, Kepler University Hospital GmbH, Johannes Kepler University, Linz, AustriaSchool of Medical Engineering and Applied Social Science, University of Applied Sciences Upper Austria, Linz, Austria; Corresponding author.The success of cell-based therapies strongly depends on the regenerative capacity of patient-derived cells, which can vary widely. Enhancing cell potency is therefore critical, especially for autologous applications. Biophysical treatment e.g. extracorporeal shockwave therapy (ESWT) has emerged as a promising tool to enhance the regenerative potential of cells and has been applied in clinical practice for the treatment of several diseases. We developed a novel, low-cost, small and adaptable multi-mode pulse generating system (PGS) that enables direct treatment of cells in 3D-printed microfluidic devices. Adipose-derived cell treatment by our novel PGS showed first promising results, including significantly increased cellular adenosine triphosphate (ATP) release and proliferation. Enhanced cell functionality could be observed through a significantly increased adipogenic differentiation potential and a trend towards osteogenic and chondrogenic lineages. This novel approach offers unique characteristics achieved by its small dimensions and light weight that come along with increased flexibility and high integrability in existing systems and could therefore overcome limitations faced by conventional biophysical methods. It enables the combination of the process of cell treatment and live monitoring of cells and could therefore emerge in the field of bioprinting, in lab-on-a-chip applications as well as future clinical applications in cell-based therapies for many different therapeutic fields.http://www.sciencedirect.com/science/article/pii/S2666496825000147Pulse generating system (PGS)Microfluidic devicesExtracorporeal shockwave therapy (ESWT)Adipose-derived cellsTherapeutic potential |
| spellingShingle | Marlene Wahlmueller Bianca Buchegger Cyrill Slezak Heinz Redl Susanne Wolbank Eleni Priglinger Armin Hochreiner Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidics Applications in Engineering Science Pulse generating system (PGS) Microfluidic devices Extracorporeal shockwave therapy (ESWT) Adipose-derived cells Therapeutic potential |
| title | Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidics |
| title_full | Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidics |
| title_fullStr | Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidics |
| title_full_unstemmed | Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidics |
| title_short | Low-cost pulse generating system for activating adipose-derived cells in 3D-printed microfluidics |
| title_sort | low cost pulse generating system for activating adipose derived cells in 3d printed microfluidics |
| topic | Pulse generating system (PGS) Microfluidic devices Extracorporeal shockwave therapy (ESWT) Adipose-derived cells Therapeutic potential |
| url | http://www.sciencedirect.com/science/article/pii/S2666496825000147 |
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