Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis
Background. High-producer TGFβ1 genotypes are associated with severe lung disease in cystic fibrosis (CF), but studies combining IL-8, TNFα-, and TGFβ1(+genotype) levels and their impact on CF lung disease are scarce. Aim. Assessing the relationship between TGFβ1, IL-8, and TNF-α and lung disease in...
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| Format: | Article |
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Wiley
2013-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/913135 |
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| author | O. Eickmeier L. v. d. Boom F. Schreiner M. J. Lentze D. NGampolo R. Schubert S. Zielen S. Schmitt-Grohé |
| author_facet | O. Eickmeier L. v. d. Boom F. Schreiner M. J. Lentze D. NGampolo R. Schubert S. Zielen S. Schmitt-Grohé |
| author_sort | O. Eickmeier |
| collection | DOAJ |
| description | Background. High-producer TGFβ1 genotypes are associated with severe lung disease in cystic fibrosis (CF), but studies combining IL-8, TNFα-, and TGFβ1(+genotype) levels and their impact on CF lung disease are scarce. Aim. Assessing the relationship between TGFβ1, IL-8, and TNF-α and lung disease in CF in an exacerbation-free interval. Methods. Twenty four patients delta F508 homozygous (median age 20.5 y, Shwachman score 75, FEV1(%) 83) and 8 controls (median age 27.5 y) were examined. TGFβ1 was assessed in serum and induced sputum (IS) by ELISA, for IL-8 and TNF-α by chemiluminescence in IS and whole blood. Genotyping was performed for TGFβ1 C−509T and T+869C utilizing RFLP. Results. TGFβ1 in IS (CF/controls median 76.5/59.1 pg/mL, P<0.074) was higher in CF. There was a negative correlation between TGFβ1 in serum and lung function (LF) (FEV1 (r=−0.488, P=0.025), MEF 25 (r=−0.425, P=0.055), and VC (r=−0.572, P=0.007)). Genotypes had no impact on TGFβ1 in IS, serum, and LF. In IS TGFβ1 correlated with IL-8 (r=0.593, P<0.007) and TNF-α (r=0.536, P<0.018) in patients colonized by bacteria with flagellin. Conclusion. TGFβ1 in serum not in IS correlates with LF. In patients colonized by bacteria with flagellin, TGFβ1 correlates with IL-8 and TNF-α in IS. |
| format | Article |
| id | doaj-art-9110f2602a014133af60759d8610ec85 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-9110f2602a014133af60759d8610ec852025-08-20T02:09:42ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/913135913135Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic FibrosisO. Eickmeier0L. v. d. Boom1F. Schreiner2M. J. Lentze3D. NGampolo4R. Schubert5S. Zielen6S. Schmitt-Grohé7Department of Pediatric Pulmonary, Goethe University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt, GermanyDepartment of Pediatrics, University Hospital, Adenauerallee 119, 53113 Bonn, GermanyDepartment of Pediatrics, University Hospital, Adenauerallee 119, 53113 Bonn, GermanyDepartment of Pediatrics, University Hospital, Adenauerallee 119, 53113 Bonn, GermanyDepartment of Pediatrics, University Hospital, Adenauerallee 119, 53113 Bonn, GermanyDepartment of Pediatric Pulmonary, Goethe University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt, GermanyDepartment of Pediatric Pulmonary, Goethe University Hospital, Theodor-Stern-Kai 7, 60590 Frankfurt, GermanyDepartment of Pediatrics, University Hospital, Adenauerallee 119, 53113 Bonn, GermanyBackground. High-producer TGFβ1 genotypes are associated with severe lung disease in cystic fibrosis (CF), but studies combining IL-8, TNFα-, and TGFβ1(+genotype) levels and their impact on CF lung disease are scarce. Aim. Assessing the relationship between TGFβ1, IL-8, and TNF-α and lung disease in CF in an exacerbation-free interval. Methods. Twenty four patients delta F508 homozygous (median age 20.5 y, Shwachman score 75, FEV1(%) 83) and 8 controls (median age 27.5 y) were examined. TGFβ1 was assessed in serum and induced sputum (IS) by ELISA, for IL-8 and TNF-α by chemiluminescence in IS and whole blood. Genotyping was performed for TGFβ1 C−509T and T+869C utilizing RFLP. Results. TGFβ1 in IS (CF/controls median 76.5/59.1 pg/mL, P<0.074) was higher in CF. There was a negative correlation between TGFβ1 in serum and lung function (LF) (FEV1 (r=−0.488, P=0.025), MEF 25 (r=−0.425, P=0.055), and VC (r=−0.572, P=0.007)). Genotypes had no impact on TGFβ1 in IS, serum, and LF. In IS TGFβ1 correlated with IL-8 (r=0.593, P<0.007) and TNF-α (r=0.536, P<0.018) in patients colonized by bacteria with flagellin. Conclusion. TGFβ1 in serum not in IS correlates with LF. In patients colonized by bacteria with flagellin, TGFβ1 correlates with IL-8 and TNF-α in IS.http://dx.doi.org/10.1155/2013/913135 |
| spellingShingle | O. Eickmeier L. v. d. Boom F. Schreiner M. J. Lentze D. NGampolo R. Schubert S. Zielen S. Schmitt-Grohé Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis Mediators of Inflammation |
| title | Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis |
| title_full | Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis |
| title_fullStr | Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis |
| title_full_unstemmed | Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis |
| title_short | Transforming Growth Factor β1 Genotypes in Relation to TGFβ1, Interleukin-8, and Tumor Necrosis Factor Alpha in Induced Sputum and Blood in Cystic Fibrosis |
| title_sort | transforming growth factor β1 genotypes in relation to tgfβ1 interleukin 8 and tumor necrosis factor alpha in induced sputum and blood in cystic fibrosis |
| url | http://dx.doi.org/10.1155/2013/913135 |
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