Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodies
Daboia russelii, a highly venomous snake prevalent in South Asia, causes significant morbidity and mortality due to its complex venom, which includes neurotoxins, hemotoxins, and reprotoxins. Current equine-derived antivenoms, while effective, are associated with adverse effects like serum sickness....
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Elsevier
2025-09-01
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| Series: | Medicine in Drug Discovery |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590098625000144 |
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| author | Punithkumar Naraganahalli Krishnaraj Kiran Kumar Mudnakudu-Nagaraju Nagalambika Prasad Chandavi Venkatesh Deekshith Ramesh Druthi Venktesh Anusha Chikkamandagere Kumar Kumar Jajur Ramanna |
| author_facet | Punithkumar Naraganahalli Krishnaraj Kiran Kumar Mudnakudu-Nagaraju Nagalambika Prasad Chandavi Venkatesh Deekshith Ramesh Druthi Venktesh Anusha Chikkamandagere Kumar Kumar Jajur Ramanna |
| author_sort | Punithkumar Naraganahalli Krishnaraj |
| collection | DOAJ |
| description | Daboia russelii, a highly venomous snake prevalent in South Asia, causes significant morbidity and mortality due to its complex venom, which includes neurotoxins, hemotoxins, and reprotoxins. Current equine-derived antivenoms, while effective, are associated with adverse effects like serum sickness. This study explores the potential of chicken-derived IgY antibodies as a safer, cost-effective alternative. Venom was fractionated using gel filtration chromatography, yielding six protein peaks, with Peak-I (large molecular weight fraction, LMF) and Peak-II (reprotoxin) used for immunization and neutralization studies. IgY antibodies raised against LMF in hens effectively neutralized twice the LD50 dose of reprotoxin (1:4 ratio) and whole venom (1:8 ratio), mitigating neurotoxicity, edema, hemolysis, anticoagulation, proteolysis, and cytotoxicity. Compared to equine antivenoms, IgY showed comparable neutralization efficacy with potentially fewer side effects, highlighting its therapeutic promise. These findings suggest IgY antibodies merit further investigation as a novel antivenom for D. russelii envenomation. |
| format | Article |
| id | doaj-art-9105956fb30a40038ad31ea35b7b26b3 |
| institution | DOAJ |
| issn | 2590-0986 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Drug Discovery |
| spelling | doaj-art-9105956fb30a40038ad31ea35b7b26b32025-08-20T03:08:17ZengElsevierMedicine in Drug Discovery2590-09862025-09-012710021710.1016/j.medidd.2025.100217Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodiesPunithkumar Naraganahalli Krishnaraj0Kiran Kumar Mudnakudu-Nagaraju1Nagalambika Prasad2Chandavi Venkatesh3Deekshith Ramesh4Druthi Venktesh5Anusha Chikkamandagere Kumar6Kumar Jajur Ramanna7Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education & Research, Mysore 570015, IndiaDepartment of Biotechnology & Bioinformatics, School of Life Sciences, JSS Academy of Higher Education & Research, Mysore 570015, IndiaDepartment of Microbiology, JSS Academy of Higher Education and Research, Mysore 570015, IndiaDivision of Biochemistry, School of Life Sciences, JSS Academy of Higher Education & Research, Mysore 570015, IndiaDivision of Biochemistry, School of Life Sciences, JSS Academy of Higher Education & Research, Mysore 570015, IndiaDivision of Biochemistry, School of Life Sciences, JSS Academy of Higher Education & Research, Mysore 570015, IndiaDivision of Biochemistry, School of Life Sciences, JSS Academy of Higher Education & Research, Mysore 570015, IndiaDivision of Biochemistry, School of Life Sciences, JSS Academy of Higher Education & Research, Mysore 570015, India; Department of Studies in Biochemistry, University of Mysore, Manasa Gangothri, Mysore 570015, India; Corresponding author at: Division of Biochemistry, School of Life Sciences, JSS Academy of Higher Education and Research, Mysore 570015, India.Daboia russelii, a highly venomous snake prevalent in South Asia, causes significant morbidity and mortality due to its complex venom, which includes neurotoxins, hemotoxins, and reprotoxins. Current equine-derived antivenoms, while effective, are associated with adverse effects like serum sickness. This study explores the potential of chicken-derived IgY antibodies as a safer, cost-effective alternative. Venom was fractionated using gel filtration chromatography, yielding six protein peaks, with Peak-I (large molecular weight fraction, LMF) and Peak-II (reprotoxin) used for immunization and neutralization studies. IgY antibodies raised against LMF in hens effectively neutralized twice the LD50 dose of reprotoxin (1:4 ratio) and whole venom (1:8 ratio), mitigating neurotoxicity, edema, hemolysis, anticoagulation, proteolysis, and cytotoxicity. Compared to equine antivenoms, IgY showed comparable neutralization efficacy with potentially fewer side effects, highlighting its therapeutic promise. These findings suggest IgY antibodies merit further investigation as a novel antivenom for D. russelii envenomation.http://www.sciencedirect.com/science/article/pii/S2590098625000144Daboia russelii venomIgY antibodiesAntivenomReprotoxinNeutralizationSnakebite |
| spellingShingle | Punithkumar Naraganahalli Krishnaraj Kiran Kumar Mudnakudu-Nagaraju Nagalambika Prasad Chandavi Venkatesh Deekshith Ramesh Druthi Venktesh Anusha Chikkamandagere Kumar Kumar Jajur Ramanna Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodies Medicine in Drug Discovery Daboia russelii venom IgY antibodies Antivenom Reprotoxin Neutralization Snakebite |
| title | Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodies |
| title_full | Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodies |
| title_fullStr | Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodies |
| title_full_unstemmed | Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodies |
| title_short | Neutralization of systemic toxicity of Daboia russelii venom and reprotoxin fraction by IgY antibodies |
| title_sort | neutralization of systemic toxicity of daboia russelii venom and reprotoxin fraction by igy antibodies |
| topic | Daboia russelii venom IgY antibodies Antivenom Reprotoxin Neutralization Snakebite |
| url | http://www.sciencedirect.com/science/article/pii/S2590098625000144 |
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