Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in Maize

Leaf angle (LA) is closely related to plant architecture, photosynthesis and density tolerance in maize. In the current study, we used a recombinant inbred line population constructed by two maize-inbred lines to detect quantitative trait loci (QTLs) controlling LA. Based on the average LA in three...

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Main Authors: Yonghui He, Chenxi Wang, Xueyou Hu, Youle Han, Feng Lu, Huanhuan Liu, Xuecai Zhang, Zhitong Yin
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/9/1978
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author Yonghui He
Chenxi Wang
Xueyou Hu
Youle Han
Feng Lu
Huanhuan Liu
Xuecai Zhang
Zhitong Yin
author_facet Yonghui He
Chenxi Wang
Xueyou Hu
Youle Han
Feng Lu
Huanhuan Liu
Xuecai Zhang
Zhitong Yin
author_sort Yonghui He
collection DOAJ
description Leaf angle (LA) is closely related to plant architecture, photosynthesis and density tolerance in maize. In the current study, we used a recombinant inbred line population constructed by two maize-inbred lines to detect quantitative trait loci (QTLs) controlling LA. Based on the average LA in three environments, 13 QTLs were detected, with the logarithm of odds ranging from 2.7 to 7.21, and the phenotypic variation explained by a single QTL ranged from 3.93% to 12.64%. A stable QTL, <i>qLA2-3</i>, on chromosome 2 was detected and was considered to be the major QTL controlling the LA. On the basis of verifying the genetic effect of <i>qLA2-3</i>, a fine map was used to narrow the candidate interval, and finally, the target segment was located at a physical distance of approximately 338.46 kb (B73 RefGen_v4 version), containing 16 genes. Re-sequencing and transcriptome results revealed that five candidate genes may be involved in the regulation of LA. The results enrich the information for molecular marker-assisted selection of maize LA and provide genetic resources for the breeding of dense planting varieties.
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spelling doaj-art-91024862d56e4c3db4cf89870b26f37a2025-08-20T01:56:02ZengMDPI AGAgronomy2073-43952024-09-01149197810.3390/agronomy14091978Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in MaizeYonghui He0Chenxi Wang1Xueyou Hu2Youle Han3Feng Lu4Huanhuan Liu5Xuecai Zhang6Zhitong Yin7Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaInternational Maize and Wheat Improvement Center (CIMMYT), Mexico City 06600, MexicoJiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genetics and Physiology/Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education, Agricultural College, Yangzhou University, Yangzhou 225009, ChinaLeaf angle (LA) is closely related to plant architecture, photosynthesis and density tolerance in maize. In the current study, we used a recombinant inbred line population constructed by two maize-inbred lines to detect quantitative trait loci (QTLs) controlling LA. Based on the average LA in three environments, 13 QTLs were detected, with the logarithm of odds ranging from 2.7 to 7.21, and the phenotypic variation explained by a single QTL ranged from 3.93% to 12.64%. A stable QTL, <i>qLA2-3</i>, on chromosome 2 was detected and was considered to be the major QTL controlling the LA. On the basis of verifying the genetic effect of <i>qLA2-3</i>, a fine map was used to narrow the candidate interval, and finally, the target segment was located at a physical distance of approximately 338.46 kb (B73 RefGen_v4 version), containing 16 genes. Re-sequencing and transcriptome results revealed that five candidate genes may be involved in the regulation of LA. The results enrich the information for molecular marker-assisted selection of maize LA and provide genetic resources for the breeding of dense planting varieties.https://www.mdpi.com/2073-4395/14/9/1978leaf anglemaizequantitative trait lociQTL mapping
spellingShingle Yonghui He
Chenxi Wang
Xueyou Hu
Youle Han
Feng Lu
Huanhuan Liu
Xuecai Zhang
Zhitong Yin
Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in Maize
Agronomy
leaf angle
maize
quantitative trait loci
QTL mapping
title Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in Maize
title_full Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in Maize
title_fullStr Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in Maize
title_full_unstemmed Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in Maize
title_short Genetic Basis and Exploration of Major Expressed QTL <i>qLA2-3</i> Underlying Leaf Angle in Maize
title_sort genetic basis and exploration of major expressed qtl i qla2 3 i underlying leaf angle in maize
topic leaf angle
maize
quantitative trait loci
QTL mapping
url https://www.mdpi.com/2073-4395/14/9/1978
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