Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and Monitoring
Common bean production is crucial in Western Kenya due to its economic, nutritional, environmental, and cultural importance. However, challenges such as diseases, especially viral diseases, cause significant crop losses. This study sought to identify potential biomarkers for BCMV and BCMNV viral dis...
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| Language: | English |
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MDPI AG
2025-04-01
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| Series: | International Journal of Plant Biology |
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| Online Access: | https://www.mdpi.com/2037-0164/16/2/43 |
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| author | Aggrey Keya Osogo Clabe Wekesa Francis N. Muyekho Hassan Karakacha Were Patrick Okoth |
| author_facet | Aggrey Keya Osogo Clabe Wekesa Francis N. Muyekho Hassan Karakacha Were Patrick Okoth |
| author_sort | Aggrey Keya Osogo |
| collection | DOAJ |
| description | Common bean production is crucial in Western Kenya due to its economic, nutritional, environmental, and cultural importance. However, challenges such as diseases, especially viral diseases, cause significant crop losses. This study sought to identify potential biomarkers for BCMV and BCMNV viral diseases by analyzing small molecule metabolites in diseased common bean systems and gain an understanding of related metabolic pathways. Virus-free Rosecoco bean cultivars were planted and exposed to BCMV and BCMNV in specific regions, with healthy plants serving as controls. Diseased and healthy leaves were collected for metabolite extraction and analyzed using liquid chromatography and mass spectrometry. A total of 354 metabolites were identified across seven pathways, with 51 upregulated metabolites, primarily from fatty acids, terpenoids, and alkaloids. Ten metabolites were differentially expressed, with the molecular structures of two successfully determined. These metabolites serve as potential biomarkers for viral disease detection, monitoring, and resistance in common beans. The findings highlight the role of fatty acids and terpenoids, as well as the importance of regional variability in plant hormone regulation in response to stress, suggesting that further research into these pathways will be essential for understanding plant defense mechanisms. |
| format | Article |
| id | doaj-art-90fd4bd28e284f39af793fcd0ff75421 |
| institution | Kabale University |
| issn | 2037-0164 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Plant Biology |
| spelling | doaj-art-90fd4bd28e284f39af793fcd0ff754212025-08-20T03:24:33ZengMDPI AGInternational Journal of Plant Biology2037-01642025-04-011624310.3390/ijpb16020043Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and MonitoringAggrey Keya Osogo0Clabe Wekesa1Francis N. Muyekho2Hassan Karakacha Were3Patrick Okoth4Department of Biological Sciences, Masinde Muliro University of Science and Technology, Kakamega P.O. Box 190-50100, KenyaMax Planck Institute for Chemical Ecology, D-07745 Jena, GermanyDepartment of Agriculture and Land Use Management, Masinde Muliro University of Science and Technology, Kakamega 50100, KenyaDepartment of Agriculture and Land Use Management, Masinde Muliro University of Science and Technology, Kakamega 50100, KenyaDepartment of Biological Sciences, Masinde Muliro University of Science and Technology, Kakamega P.O. Box 190-50100, KenyaCommon bean production is crucial in Western Kenya due to its economic, nutritional, environmental, and cultural importance. However, challenges such as diseases, especially viral diseases, cause significant crop losses. This study sought to identify potential biomarkers for BCMV and BCMNV viral diseases by analyzing small molecule metabolites in diseased common bean systems and gain an understanding of related metabolic pathways. Virus-free Rosecoco bean cultivars were planted and exposed to BCMV and BCMNV in specific regions, with healthy plants serving as controls. Diseased and healthy leaves were collected for metabolite extraction and analyzed using liquid chromatography and mass spectrometry. A total of 354 metabolites were identified across seven pathways, with 51 upregulated metabolites, primarily from fatty acids, terpenoids, and alkaloids. Ten metabolites were differentially expressed, with the molecular structures of two successfully determined. These metabolites serve as potential biomarkers for viral disease detection, monitoring, and resistance in common beans. The findings highlight the role of fatty acids and terpenoids, as well as the importance of regional variability in plant hormone regulation in response to stress, suggesting that further research into these pathways will be essential for understanding plant defense mechanisms.https://www.mdpi.com/2037-0164/16/2/43<i>Phaseolus vulgaris</i> L.metabolomic profilingbiomarkersdisease monitoring and detection |
| spellingShingle | Aggrey Keya Osogo Clabe Wekesa Francis N. Muyekho Hassan Karakacha Were Patrick Okoth Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and Monitoring International Journal of Plant Biology <i>Phaseolus vulgaris</i> L. metabolomic profiling biomarkers disease monitoring and detection |
| title | Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and Monitoring |
| title_full | Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and Monitoring |
| title_fullStr | Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and Monitoring |
| title_full_unstemmed | Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and Monitoring |
| title_short | Comprehensive Metabolomic Profiling of Common Bean (<i>Phaseolus vulgaris</i> L.) Reveals Biomarkers Involved in Viral Disease Detection and Monitoring |
| title_sort | comprehensive metabolomic profiling of common bean i phaseolus vulgaris i l reveals biomarkers involved in viral disease detection and monitoring |
| topic | <i>Phaseolus vulgaris</i> L. metabolomic profiling biomarkers disease monitoring and detection |
| url | https://www.mdpi.com/2037-0164/16/2/43 |
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