Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research Protocol
Introduction: Cancer Stem Cells (CSCs), known for their self-renewal and resistance to therapy, drive tumour progression, metastasis, and recurrence. Markers such as Cluster of Differentiation 133 (CD133) and CXC Chemokine Receptor-4 (CXCR-4) are linked to poor prognosis in cancers, including Oral S...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2025-06-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/21101/75424_CE[Ra1]_F(IS)_QC_PF1(HJ_SS)_PFA(IS)_PN(IS).pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850223018465296384 |
|---|---|
| author | Nirlipta Swain Arvind Shridhar Bhake |
| author_facet | Nirlipta Swain Arvind Shridhar Bhake |
| author_sort | Nirlipta Swain |
| collection | DOAJ |
| description | Introduction: Cancer Stem Cells (CSCs), known for their self-renewal and resistance to therapy, drive tumour progression, metastasis, and recurrence. Markers such as Cluster of Differentiation 133 (CD133) and CXC Chemokine Receptor-4 (CXCR-4) are linked to poor prognosis in cancers, including Oral Squamous Cell Carcinoma (OSCC). CD133 promotes Epithelial-Mesenchymal Transition (EMT) and chemoresistance, while CXCR-4 enhances invasion via CXCL12 signalling. Their co-expression exacerbates outcomes; however, region-specific data, particularly from high-incidence areas like India, remain scarce.
Need of the study: The expression and prognostic correlation of CXCR-4 and CD133 in resectable OSCC enhance early detection, assess tumour aggressiveness, and may identify potential therapeutic targets.
Aim: This study aims to evaluate the immunohistochemical expression of CXCR-4 and CD133 in resectable OSCC and analyse their correlation with key prognostic pathological factors, including tumour grade, tumour size (T-stage), Depth of Invasion (DOI), Lymphovascular Invasion (LVI), Perineural Invasion (PNI), lymph node metastasis (N-stage), and Lymph node metastasis and surgical margin status.
Materials and Methods: This observational, cross-sectional study will be conducted at Jawaharlal Nehru Medical College, DMIHER. Seventy-five OSCC tissue samples will undergo immunohistochemical analysis using monoclonal antibodies against CXCR-4 and CD133. The expression levels of these markers will be assessed semi-quantitatively. Subsequently, their correlation with key prognostic pathological factors will be analysed. Statistical analysis will be performed using SPSS version 27.0, with a p-value of less than 0.05 considered statistically significant. |
| format | Article |
| id | doaj-art-90fb1cec64cd4a4fa441fbc74b392141 |
| institution | OA Journals |
| issn | 2249-782X 0973-709X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj-art-90fb1cec64cd4a4fa441fbc74b3921412025-08-20T02:06:06ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2025-06-01196EK01EK0410.7860/JCDR/2025/75424.21101Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research ProtocolNirlipta Swain0Arvind Shridhar Bhake1Junior Resident, Department of Pathology, Jawaharlal Nehru Medical College, Sawangi (Meghe), Wardha, Maharashtra, India.Director and Professor, Department of Pathology, Jawaharlal Nehru Medical College, Sawangi (Meghe), Wardha, Maharashtra, India.Introduction: Cancer Stem Cells (CSCs), known for their self-renewal and resistance to therapy, drive tumour progression, metastasis, and recurrence. Markers such as Cluster of Differentiation 133 (CD133) and CXC Chemokine Receptor-4 (CXCR-4) are linked to poor prognosis in cancers, including Oral Squamous Cell Carcinoma (OSCC). CD133 promotes Epithelial-Mesenchymal Transition (EMT) and chemoresistance, while CXCR-4 enhances invasion via CXCL12 signalling. Their co-expression exacerbates outcomes; however, region-specific data, particularly from high-incidence areas like India, remain scarce. Need of the study: The expression and prognostic correlation of CXCR-4 and CD133 in resectable OSCC enhance early detection, assess tumour aggressiveness, and may identify potential therapeutic targets. Aim: This study aims to evaluate the immunohistochemical expression of CXCR-4 and CD133 in resectable OSCC and analyse their correlation with key prognostic pathological factors, including tumour grade, tumour size (T-stage), Depth of Invasion (DOI), Lymphovascular Invasion (LVI), Perineural Invasion (PNI), lymph node metastasis (N-stage), and Lymph node metastasis and surgical margin status. Materials and Methods: This observational, cross-sectional study will be conducted at Jawaharlal Nehru Medical College, DMIHER. Seventy-five OSCC tissue samples will undergo immunohistochemical analysis using monoclonal antibodies against CXCR-4 and CD133. The expression levels of these markers will be assessed semi-quantitatively. Subsequently, their correlation with key prognostic pathological factors will be analysed. Statistical analysis will be performed using SPSS version 27.0, with a p-value of less than 0.05 considered statistically significant.https://jcdr.net/articles/PDF/21101/75424_CE[Ra1]_F(IS)_QC_PF1(HJ_SS)_PFA(IS)_PN(IS).pdfbiomarkerschemokineepithelial-mesenchymal transitiongene expression regulationimmunohistochemistrylymphatic metastasistumour microenvironment |
| spellingShingle | Nirlipta Swain Arvind Shridhar Bhake Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research Protocol Journal of Clinical and Diagnostic Research biomarkers chemokine epithelial-mesenchymal transition gene expression regulation immunohistochemistry lymphatic metastasis tumour microenvironment |
| title | Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research Protocol |
| title_full | Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research Protocol |
| title_fullStr | Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research Protocol |
| title_full_unstemmed | Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research Protocol |
| title_short | Expression of CXCR-4 and CD 133 and it’s Correlation with Prognostic Pathologic Factors in Resectable Oral Squamous Cell Carcinoma: A Research Protocol |
| title_sort | expression of cxcr 4 and cd 133 and it s correlation with prognostic pathologic factors in resectable oral squamous cell carcinoma a research protocol |
| topic | biomarkers chemokine epithelial-mesenchymal transition gene expression regulation immunohistochemistry lymphatic metastasis tumour microenvironment |
| url | https://jcdr.net/articles/PDF/21101/75424_CE[Ra1]_F(IS)_QC_PF1(HJ_SS)_PFA(IS)_PN(IS).pdf |
| work_keys_str_mv | AT nirliptaswain expressionofcxcr4andcd133anditscorrelationwithprognosticpathologicfactorsinresectableoralsquamouscellcarcinomaaresearchprotocol AT arvindshridharbhake expressionofcxcr4andcd133anditscorrelationwithprognosticpathologicfactorsinresectableoralsquamouscellcarcinomaaresearchprotocol |