The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins
Peripheral neuropathy (PN) is a prevalent condition characterized by damage to peripheral nerves, often linked to risk factors such as diabetes. This condition results from various forms of neural damage, including injury to the cell body, axons, or demyelination, frequently beginning with small and...
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MDPI AG
2025-03-01
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| author | Oscar Cuyubamba Camila Pereira Braga Dionne Swift John T. Stickney Christian Viel |
| author_facet | Oscar Cuyubamba Camila Pereira Braga Dionne Swift John T. Stickney Christian Viel |
| author_sort | Oscar Cuyubamba |
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| description | Peripheral neuropathy (PN) is a prevalent condition characterized by damage to peripheral nerves, often linked to risk factors such as diabetes. This condition results from various forms of neural damage, including injury to the cell body, axons, or demyelination, frequently beginning with small and thinly or unmyelinated fibers. Such nerve damage disrupts normal signaling, leading to symptoms like numbness, tingling, and pain. Effective nerve repair and regeneration, particularly through remyelination, are essential therapeutic objectives. While vitamin B12’s role in repair processes has been well established, emerging evidence suggests that other neurotropic vitamins, specifically B1 and B6, also contribute significantly to nerve health and symptom relief in PN. In this study, we demonstrate that a combination treatment of vitamins B1, B6, and B12 enhances repair and oxidative stress responses in co-cultures of neural and Schwann cells, leading to improved cell maturation and connectivity compared to vitamin B12 alone. Furthermore, proteomic analysis supports these observations at the molecular level, with enhanced cellular recycling processes like proteasome enhancement, as well as protein synthesis upregulation, needed to rebuild nerve connections and combatting oxidative stress. Our combined morphological and molecular results highlight the potential therapeutic advantage of the B1, B6, and B12 combination over vitamin B12 alone. |
| format | Article |
| id | doaj-art-90ef71c735714c06a7e3edf032fe2d63 |
| institution | DOAJ |
| issn | 2073-4409 |
| language | English |
| publishDate | 2025-03-01 |
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| series | Cells |
| spelling | doaj-art-90ef71c735714c06a7e3edf032fe2d632025-08-20T03:08:45ZengMDPI AGCells2073-44092025-03-0114747710.3390/cells14070477The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B VitaminsOscar Cuyubamba0Camila Pereira Braga1Dionne Swift2John T. Stickney3Christian Viel4The Procter & Gamble Company, Mason Business and Innovation Center, 8700 Mason Montgomery Road, Mason, OH 45040, USAThe Procter & Gamble Company, Mason Business and Innovation Center, 8700 Mason Montgomery Road, Mason, OH 45040, USAThe Procter & Gamble Company, Mason Business and Innovation Center, 8700 Mason Montgomery Road, Mason, OH 45040, USAThe Procter & Gamble Company, Mason Business and Innovation Center, 8700 Mason Montgomery Road, Mason, OH 45040, USAP&G Health Germany GmbH, German Innovation Center, Sulzbacher Straße 40, 65824 Schwalbach am Taunus, GermanyPeripheral neuropathy (PN) is a prevalent condition characterized by damage to peripheral nerves, often linked to risk factors such as diabetes. This condition results from various forms of neural damage, including injury to the cell body, axons, or demyelination, frequently beginning with small and thinly or unmyelinated fibers. Such nerve damage disrupts normal signaling, leading to symptoms like numbness, tingling, and pain. Effective nerve repair and regeneration, particularly through remyelination, are essential therapeutic objectives. While vitamin B12’s role in repair processes has been well established, emerging evidence suggests that other neurotropic vitamins, specifically B1 and B6, also contribute significantly to nerve health and symptom relief in PN. In this study, we demonstrate that a combination treatment of vitamins B1, B6, and B12 enhances repair and oxidative stress responses in co-cultures of neural and Schwann cells, leading to improved cell maturation and connectivity compared to vitamin B12 alone. Furthermore, proteomic analysis supports these observations at the molecular level, with enhanced cellular recycling processes like proteasome enhancement, as well as protein synthesis upregulation, needed to rebuild nerve connections and combatting oxidative stress. Our combined morphological and molecular results highlight the potential therapeutic advantage of the B1, B6, and B12 combination over vitamin B12 alone.https://www.mdpi.com/2073-4409/14/7/477neurotropic B vitaminsnerve regenerationperipheral neuropathyproteomics |
| spellingShingle | Oscar Cuyubamba Camila Pereira Braga Dionne Swift John T. Stickney Christian Viel The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins Cells neurotropic B vitamins nerve regeneration peripheral neuropathy proteomics |
| title | The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins |
| title_full | The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins |
| title_fullStr | The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins |
| title_full_unstemmed | The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins |
| title_short | The Combination of Neurotropic Vitamins B1, B6, and B12 Enhances Neural Cell Maturation and Connectivity Superior to Single B Vitamins |
| title_sort | combination of neurotropic vitamins b1 b6 and b12 enhances neural cell maturation and connectivity superior to single b vitamins |
| topic | neurotropic B vitamins nerve regeneration peripheral neuropathy proteomics |
| url | https://www.mdpi.com/2073-4409/14/7/477 |
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