Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae)
This study aims to determine the anti-aging effects of Achillea phrygia, an endemic plant, by evaluating its sun protection factor (SPF) level, antioxidant activity, total phenolic content, extracellular matrix-degrading enzymes (ECM) inhibition, genotoxic/anti-genotoxic, and cytotoxic activities. T...
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2024-11-01
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| Series: | International Journal of Secondary Metabolite |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/3585358 |
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| author | Aysel Uğur Nurdan Saraç Rukiye Boran Gülen |
| author_facet | Aysel Uğur Nurdan Saraç Rukiye Boran Gülen |
| author_sort | Aysel Uğur |
| collection | DOAJ |
| description | This study aims to determine the anti-aging effects of Achillea phrygia, an endemic plant, by evaluating its sun protection factor (SPF) level, antioxidant activity, total phenolic content, extracellular matrix-degrading enzymes (ECM) inhibition, genotoxic/anti-genotoxic, and cytotoxic activities. The SPF level was assessed using an in vitro quantitative method, while antioxidant capacity was determined through DPPH, β-carotene, and hydroxyl-radical (H2O2) scavenging assays. The total phenolic content was quantitatively conducted using the Folin Ciocalteu reagent. The inhibition of ECM-degrading enzymes was determined using matrix metalloproteinase-1 (MMP-1), hyaluronidase, and elastase enzymes. Genotoxic/anti-genotoxic properties were assessed using the AMES Salmonella/microsome assay, and cytotoxicity effects were assessed through the MTT assay. The results indicated that A. phrygia showed moderate SPF activity (SPF = 4.013) and exhibited IC50 values of 0.183 ± 0.03, 0.079 ± 0.51, and 1.18 ± 0.35 mg/mL for DPPH, β-carotene, and hydroxyl-radicals, respectively. The total phenolic content was measured to be 23.56 ± 1.42 mg GAE/g dry extract. Furthermore, the extract demonstrated inhibition of MMP-1 (47.98%) and elastase (39.2%) activities. Importantly, it did not induce DNA damage and showed antigenotoxic activity ranging from 10% to 65.6%. The cytotoxicity assay revealed an IC50 value of 42.41±4.05 µg/mL. These findings suggest that A. phrygia could be utilized as a cosmetic ingredient in skincare products due to its ability to protect against UV radiation, exhibit antioxidant properties, prevent extracellular matrix degradation, and inhibit DNA damage. |
| format | Article |
| id | doaj-art-90ee0364c7e3475086c8efbbc4804abc |
| institution | OA Journals |
| issn | 2148-6905 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | izzet kara |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj-art-90ee0364c7e3475086c8efbbc4804abc2025-08-20T01:58:27Zengizzet karaInternational Journal of Secondary Metabolite2148-69052024-11-0111464665710.21448/ijsm.1401681618Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae)Aysel Uğur0https://orcid.org/0000-0002-5188-1106Nurdan Saraç1https://orcid.org/0000-0001-7676-542XRukiye Boran Gülen2https://orcid.org/0000-0003-2395-2445GAZI UNIVERSITYMUGLA SITKI KOCMAN UNIVERSITYAKSARAY UNIVERSITYThis study aims to determine the anti-aging effects of Achillea phrygia, an endemic plant, by evaluating its sun protection factor (SPF) level, antioxidant activity, total phenolic content, extracellular matrix-degrading enzymes (ECM) inhibition, genotoxic/anti-genotoxic, and cytotoxic activities. The SPF level was assessed using an in vitro quantitative method, while antioxidant capacity was determined through DPPH, β-carotene, and hydroxyl-radical (H2O2) scavenging assays. The total phenolic content was quantitatively conducted using the Folin Ciocalteu reagent. The inhibition of ECM-degrading enzymes was determined using matrix metalloproteinase-1 (MMP-1), hyaluronidase, and elastase enzymes. Genotoxic/anti-genotoxic properties were assessed using the AMES Salmonella/microsome assay, and cytotoxicity effects were assessed through the MTT assay. The results indicated that A. phrygia showed moderate SPF activity (SPF = 4.013) and exhibited IC50 values of 0.183 ± 0.03, 0.079 ± 0.51, and 1.18 ± 0.35 mg/mL for DPPH, β-carotene, and hydroxyl-radicals, respectively. The total phenolic content was measured to be 23.56 ± 1.42 mg GAE/g dry extract. Furthermore, the extract demonstrated inhibition of MMP-1 (47.98%) and elastase (39.2%) activities. Importantly, it did not induce DNA damage and showed antigenotoxic activity ranging from 10% to 65.6%. The cytotoxicity assay revealed an IC50 value of 42.41±4.05 µg/mL. These findings suggest that A. phrygia could be utilized as a cosmetic ingredient in skincare products due to its ability to protect against UV radiation, exhibit antioxidant properties, prevent extracellular matrix degradation, and inhibit DNA damage.https://dergipark.org.tr/en/download/article-file/3585358achillea phrygiaskin-careantioxidantenzyme inhibitionanti-genotoxicityachillea phrygiaskin-careantioxidantenzyme inhibitionanti-genotoxicity |
| spellingShingle | Aysel Uğur Nurdan Saraç Rukiye Boran Gülen Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae) International Journal of Secondary Metabolite achillea phrygia skin-care antioxidant enzyme inhibition anti-genotoxicity achillea phrygia skin-care antioxidant enzyme inhibition anti-genotoxicity |
| title | Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae) |
| title_full | Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae) |
| title_fullStr | Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae) |
| title_full_unstemmed | Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae) |
| title_short | Evaluation of potential anti-aging effects of Achillea phrygia Boiss. & Balansa (Asteraceae) |
| title_sort | evaluation of potential anti aging effects of achillea phrygia boiss balansa asteraceae |
| topic | achillea phrygia skin-care antioxidant enzyme inhibition anti-genotoxicity achillea phrygia skin-care antioxidant enzyme inhibition anti-genotoxicity |
| url | https://dergipark.org.tr/en/download/article-file/3585358 |
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